##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AvrII BciVI BlnI BsiWI
Bsp1407I BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII KpnI KspI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I RcaI RsrII SacII
SalI ScaI SexAI SfuI SgrAI
SnaBI SphI SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 2066 TCCGGA T^CCGGA
AcsI 7434 GAATTT [GA]^AATT[CT]
Aor51HI 1244 AGCGCT AGC^GCT
ApoI 7434 GAATTT [GA]^AATT[CT]
Asp700I 5608 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2569 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2621 GGATCC G^GATCC
BbrPI 1429 CACGTG CAC^GTG
BclI 3647 TGATCA T^GATCA
BglII 4592 AGATCT A^GATCT
BsaAI 1429 CACGTG [CT]AC^GT[GA]
BsaBI 3642 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2066 TCCGGA T^CCGGA
BspDI 3673 ATCGAT AT^CGAT
BspEI 2066 TCCGGA T^CCGGA
BsrFI 5063 GCCGGC [GA]^CCGG[CT]
Cfr10I 5063 GCCGGC [GA]^CCGG[CT]
ClaI 3673 ATCGAT AT^CGAT
DraI 7441 TTTAAA TTT^AAA
DraIII 3073 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6725 GACCCTCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4413 CGGCCG C^GGCCG
EclXI 4413 CGGCCG C^GGCCG
Eco47III 1244 AGCGCT AGC^GCT
Eco52I 4413 CGGCCG C^GGCCG
FbaI 3647 TGATCA T^GATCA
HpaI 6554 GTTAAC GTT^AAC
MamI 3642 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2066 TCCGGA T^CCGGA
NaeI 5063 GCCGGC GCC^GGC
NgoMIV 5063 GCCGGC G^CCGGC
NheI 5050 GCTAGC G^CTAGC
PaeR7I 1 CTCGAG C^TCGAG
PflFI 2569 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1429 CACGTG CAC^GTG
PmlI 1429 CACGTG CAC^GTG
PvuI 1443 CGATCG CGAT^CG
SbfI 6460 CCTGCAGG CCTGCA^GG
SpeI 6757 ACTAGT A^CTAGT
Sse8387I 6460 CCTGCAGG CCTGCA^GG
Tth111I 2569 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6632 TCTAGA T^CTAGA
XhoI 1 CTCGAG C^TCGAG
XmnI 5608 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AccI 2133 GTCTAC GT^[AC][GT]AC
6753 GTCTAC
AflII 1258 CTTAAG C^TTAAG
6617 CTTAAG
AviII 1502 TGCGCA TGC^GCA
3830 TGCGCA
BfrI 1258 CTTAAG C^TTAAG
6617 CTTAAG
BsiEI 1443 CGATCG CG[GA][CT]^CG
4413 CGGCCG
BsmBI 1034 GAGACG CGTCTC(1/5)
1516 GAGACG
BsrDI 1353 CATTGC GCAATG(2/0)
4748 CATTGC
BssSI 4090 CACGAG CACGAG(-5/-1)
4402 CTCGTG
FspI 1502 TGCGCA TGC^GCA
3830 TGCGCA
HincII 3336 GTTGAC GT[CT]^[GA]AC
6554 GTTAAC
HindII 3336 GTTGAC GT[CT]^[GA]AC
6554 GTTAAC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI ApaI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI