##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AscI Asp718I AspEI AspI
AvrII BamHI BclI BfrI BlnI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eam1105I EcoRV
FbaI HincII HindII HpaI KpnI
KspI MroI NheI NotI NruI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SfuI SnaBI SpeI SspBI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1508 GACGTC GACGT^C
AccI 5748 GTCTAC GT^[AC][GT]AC
Alw44I 4582 GTGCAC G^TGCAC
Aor51HI 1987 AGCGCT AGC^GCT
ApaLI 4582 GTGCAC G^TGCAC
AseI 6205 ATTAAT AT^TAAT
AsnI 6205 ATTAAT AT^TAAT
Asp700I 3106 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsaBI 1867 GATCGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 3301 TCATGA T^CATGA
BspLU11I 4554 ACATGT A^CATGT
BssHII 43 GCGCGC G^CGCGC
BstAPI 1027 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 1987 AGCGCT AGC^GCT
EcoRI 5879 GAATTC G^AATTC
EcoT22I 6089 ATGCAT ATGCA^T
FseI 1666 GGCCGGCC GGCCGG^CC
MamI 1867 GATCGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4169 CAATTG C^AATTG
MluI 86 ACGCGT A^CGCGT
MunI 4169 CAATTG C^AATTG
NsiI 6089 ATGCAT ATGCA^T
PpuMI 4092 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 3332 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6205 ATTAAT AT^TAAT
RcaI 3301 TCATGA T^CATGA
SgrAI 1513 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1682 CCCGGG CCC^GGG
SspI 5966 AATATT AAT^ATT
XmaCI 1682 CCCGGG C^CCGGG
XmaI 1682 CCCGGG C^CCGGG
XmnI 3106 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BbrPI 1595 CACGTG CAC^GTG
4155 CACGTG
BcgI 1354 GCACGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2930 GCATGTTCCTCG
BciVI 3498 GTATCC GTATCC(6/5)
4799 GTATCC
BglII 678 AGATCT A^GATCT
1275 AGATCT
BsmBI 973 CGTCTC CGTCTC(1/5)
4633 GAGACG
DraI 4867 TTTAAA TTT^AAA
6078 TTTAAA
NcoI 2136 CCATGG C^CATGG
2673 CCATGG
NdeI 6086 CATATG CA^TATG
6231 CATATG
PmaCI 1595 CACGTG CAC^GTG
4155 CACGTG
PmlI 1595 CACGTG CAC^GTG
4155 CACGTG
SfiI 403 GGCCTCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2086 GGCCGAGGTGGCC
StuI 216 AGGCCT AGG^CCT
4462 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
AviII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PflMI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I SbfI
ScaI ScrFI SexAI SfaNI SfcI
SfoI SmlI SphI Sse8387I StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII