##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI BamHI BcgI BsiWI
Bsp1407I BspDI BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FseI KpnI MluI NaeI NgoMIV
NheI NotI NspV PacI PaeR7I
PflMI PshAI Psp1406I PvuI PvuII
RsrII SapI SbfI ScaI SfiI
SfuI SnaBI Sse8387I SspBI StuI
SwaI Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflII 5344 CTTAAG C^TTAAG
AgeI 4190 ACCGGT A^CCGGT
AocI 6238 CCTGAGG CC^T[ACGT]AGG
AvrII 6130 CCTAGG C^CTAGG
BfrI 5344 CTTAAG C^TTAAG
BglI 6129 GCCTAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 6130 CCTAGG C^CTAGG
BsaBI 2570 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 4701 CGGTCG CG[GA][CT]^CG
BspMI 4239 ACCTGC ACCTGC(4/8)
BsrFI 4190 ACCGGT [GA]^CCGG[CT]
BstAPI 4745 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 6238 CCTGAGG CC^T[ACGT]AGG
Cfr10I 4190 ACCGGT [GA]^CCGG[CT]
DraIII 380 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4187 GACACCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 6238 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1405 ATGCAT ATGCA^T
MamI 2570 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 305 CAATTG C^AATTG
MunI 305 CAATTG C^AATTG
NdeI 1402 CATATG CA^TATG
NruI 6481 TCGCGA TCG^CGA
NsiI 1405 ATGCAT ATGCA^T
PinAI 4190 ACCGGT A^CCGGT
PmeI 1984 GTTTAAAC GTTT^AAAC
SacI 2939 GAGCTC GAGCT^C
SalI 4703 GTCGAC G^TCGAC
SgrAI 4189 CACCGGTG C[GA]^CCGG[CT]G
SpeI 6469 ACTAGT A^CTAGT
SphI 6325 GCATGC GCATG^C
XcmI 4834 CCAGGATTCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 2563 TCCGGA T^CCGGA
3501 TCCGGA
AcyI 4153 GGCGCC G[GA]^CG[CT]C
4390 GGCGCC
AseI 823 ATTAAT AT^TAAT
3509 ATTAAT
AsnI 823 ATTAAT AT^TAAT
3509 ATTAAT
AviII 3336 TGCGCA TGC^GCA
4536 TGCGCA
BalI 1106 TGGCCA TGG^CCA
4606 TGGCCA
BanI 4153 GGCGCC G^G[CT][GA]CC
4390 GGCGCC
BbeI 4153 GGCGCC GGCGC^C
4390 GGCGCC
BciVI 3358 GGATAC GTATCC(6/5)
5428 GTATCC
BlpI 3172 GCTCAGC GC^T[ACGT]AGC
5483 GCTTAGC
Bpu1102I 3172 GCTCAGC GC^T[ACGT]AGC
5483 GCTTAGC
BsaHI 4153 GGCGCC G[GA]^CG[CT]C
4390 GGCGCC
BsaI 4236 GAGACC GGTCTC(1/5)
576 GGTCTC
BseAI 2563 TCCGGA T^CCGGA
3501 TCCGGA
BsmI 4108 GAATGC GAATGC(1/-1)
1260 GCATTC
BspEI 2563 TCCGGA T^CCGGA
3501 TCCGGA
BspHI 1451 TCATGA T^CATGA
5050 TCATGA
BspLU11I 1907 ACATGT A^CATGT
4293 ACATGT
BsrBI 2664 CCGCTC CCGCTC(-3/-3)
4396 CCGCTC
BssHII 2709 GCGCGC G^CGCGC
3250 GCGCGC
BssSI 4583 CTCGTG CACGAG(-5/-1)
4736 CTCGTG
BstEII 4420 GGTGACC G^GT[ACGT]ACC
5719 GGTCACC
BstPI 4420 GGTGACC G^GT[ACGT]ACC
5719 GGTCACC
CelII 3172 GCTCAGC GC^T[ACGT]AGC
5483 GCTTAGC
EcoO65I 4420 GGTGACC G^GT[ACGT]ACC
5719 GGTCACC
FspI 3336 TGCGCA TGC^GCA
4536 TGCGCA
Hin1I 4153 GGCGCC G[GA]^CG[CT]C
4390 GGCGCC
HpaI 610 GTTAAC GTT^AAC
5032 GTTAAC
KasI 4153 GGCGCC G^GCGCC
4390 GGCGCC
KspI 2916 CCGCGG CCGC^GG
2957 CCGCGG
MluNI 1106 TGGCCA TGG^CCA
4606 TGGCCA
MroI 2563 TCCGGA T^CCGGA
3501 TCCGGA
MscI 1106 TGGCCA TGG^CCA
4606 TGGCCA
NarI 4153 GGCGCC GG^CGCC
4390 GGCGCC
PpuMI 2808 AGGACCT [GA]G^G[AT]CC[CT]
4272 GGGACCC
PshBI 823 ATTAAT AT^TAAT
3509 ATTAAT
RcaI 1451 TCATGA T^CATGA
5050 TCATGA
SacII 2916 CCGCGG CCGC^GG
2957 CCGCGG
SexAI 3285 ACCTGGT A^CC[AT]GGT
4416 ACCTGGT
SfoI 4153 GGCGCC GGC^GCC
4390 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BanII
BbrPI BbsI BbvCI BbvI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PleI PmaCI PmlI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XbaI XhoII
XmaCI XmaI