##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AhdI AscI AspEI
AspI AviII BbrPI BciVI BclI
BglII BsaAI BsiWI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoRV FbaI FseI FspI
HpaI MfeI MluI MunI NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SbfI ScaI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 4264 GGTACC G^GTACC
AccI 3993 GTCGAC GT^[AC][GT]AC
AflIII 5316 ACATGT A^C[GA][CT]GT
AgeI 1531 ACCGGT A^CCGGT
Alw44I 879 GTGCAC G^TGCAC
ApaLI 879 GTGCAC G^TGCAC
AseI 3094 ATTAAT AT^TAAT
AsnI 3094 ATTAAT AT^TAAT
Asp718I 4264 GGTACC G^GTACC
AvrII 2268 CCTAGG C^CTAGG
BalI 4359 TGGCCA TGG^CCA
BbeI 49 GGCGCC GGCGC^C
BglI 499 GCCTGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2268 CCTAGG C^CTAGG
BsiEI 2508 CGACCG CG[GA][CT]^CG
BsmBI 4707 CGTCTC CGTCTC(1/5)
BsmI 2326 GAATGC GAATGC(1/-1)
BspHI 1493 TCATGA T^CATGA
BspLU11I 5316 ACATGT A^CATGT
BsrDI 3189 GCAATG GCAATG(2/0)
BstEII 4721 GGTGACC G^GT[ACGT]ACC
BstPI 4721 GGTGACC G^GT[ACGT]ACC
EcoO65I 4721 GGTGACC G^GT[ACGT]ACC
EcoRI 2043 GAATTC G^AATTC
EcoT22I 3472 ATGCAT ATGCA^T
HincII 3993 GTCGAC GT[CT]^[GA]AC
HindII 3993 GTCGAC GT[CT]^[GA]AC
KasI 49 GGCGCC G^GCGCC
KpnI 4264 GGTACC GGTAC^C
KspI 1446 CCGCGG CCGC^GG
MluNI 4359 TGGCCA TGG^CCA
MscI 4359 TGGCCA TGG^CCA
NarI 49 GGCGCC GG^CGCC
NdeI 4480 CATATG CA^TATG
NsiI 3472 ATGCAT ATGCA^T
PinAI 1531 ACCGGT A^CCGGT
PshBI 3094 ATTAAT AT^TAAT
RcaI 1493 TCATGA T^CATGA
SacII 1446 CCGCGG CCGC^GG
SalI 3993 GTCGAC G^TCGAC
SfiI 498 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 49 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 216 TCCGGA T^CCGGA
3734 TCCGGA
AcyI 49 GGCGCC G[GA]^CG[CT]C
5398 GGCGTC
AflII 2095 CTTAAG C^TTAAG
2298 CTTAAG
AlwNI 3645 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
5111 CAGAAACTG
Aor51HI 1064 AGCGCT AGC^GCT
4610 AGCGCT
BamHI 1940 GGATCC G^GATCC
4190 GGATCC
BbsI 1791 GAAGAC GAAGAC(2/6)
4521 GAAGAC
BbvCI 6 CCTCAGC CCTCAGC(-5/-2)
2346 CCTCAGC
BfrI 2095 CTTAAG C^TTAAG
2298 CTTAAG
BlpI 3303 GCTCAGC GC^T[ACGT]AGC
4606 GCTCAGC
Bpu1102I 3303 GCTCAGC GC^T[ACGT]AGC
4606 GCTCAGC
BpuAI 1791 GAAGAC GAAGAC(2/6)
4521 GAAGAC
BsaBI 1941 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4853 GATCAAAATC
BsaHI 49 GGCGCC G[GA]^CG[CT]C
5398 GGCGTC
BseAI 216 TCCGGA T^CCGGA
3734 TCCGGA
BspEI 216 TCCGGA T^CCGGA
3734 TCCGGA
BssSI 1753 CACGAG CACGAG(-5/-1)
1888 CTCGTG
CelII 3303 GCTCAGC GC^T[ACGT]AGC
4606 GCTCAGC
Eco47III 1064 AGCGCT AGC^GCT
4610 AGCGCT
HgaI 1409 GCGTC GACGC(5/10)
5399 GCGTC
Hin1I 49 GGCGCC G[GA]^CG[CT]C
5398 GGCGTC
MamI 1941 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4853 GATCAAAATC
MroI 216 TCCGGA T^CCGGA
3734 TCCGGA
NaeI 2719 GCCGGC GCC^GGC
5264 GCCGGC
NcoI 2652 CCATGG C^CATGG
5939 CCATGG
NgoMIV 2719 GCCGGC G^CCGGC
5264 GCCGGC
NspI 5316 ACATGT [GA]CATG^[CT]
5653 ACATGC
PflMI 1752 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5847 CCAAGAGGTGG
PstI 155 CTGCAG CTGCA^G
208 CTGCAG
SexAI 749 ACCTGGT A^CC[AT]GGT
833 ACCTGGT
SmaI 2053 CCCGGG CCC^GGG
3202 CCCGGG
Van91I 1752 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5847 CCAAGAGGTGG
XcmI 2672 CCAAAACCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5191 CCAGAAGAGGCCTGG
XmaCI 2053 CCCGGG C^CCGGG
3202 CCCGGG
XmaI 2053 CCCGGG C^CCGGG
3202 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApaI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbvI BcgI BcnI BfaI BmyI
BpmI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SgrAI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI