##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AvrII BalI BamHI
BbeI BcgI BlnI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRI EcoRV FseI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflII 2408 CTTAAG C^TTAAG
Alw44I 2586 GTGCAC G^TGCAC
AocI 3720 CCTCAGG CC^T[ACGT]AGG
ApaI 55 GGGCCC GGGCC^C
ApaLI 2586 GTGCAC G^TGCAC
AspI 3359 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 471 TGCGCA TGC^GCA
BbrPI 3209 CACGTG CAC^GTG
BbvCI 482 GCTGAGG CCTCAGC(-5/-2)
BciVI 2344 GGATAC GTATCC(6/5)
BclI 742 TGATCA T^GATCA
BfrI 2408 CTTAAG C^TTAAG
BglI 264 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2679 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2679 GCTCAGC GC^T[ACGT]AGC
BsaAI 3209 CACGTG [CT]AC^GT[GA]
BsaBI 3434 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2878 GAATGC GAATGC(1/-1)
Bsp1407I 3205 TGTACA T^GTACA
BspLU11I 2089 ACATGT A^CATGT
BsrGI 3205 TGTACA T^GTACA
BstEII 2155 GGTAACC G^GT[ACGT]ACC
BstPI 2155 GGTAACC G^GT[ACGT]ACC
Bsu36I 3720 CCTCAGG CC^T[ACGT]AGG
CelII 2679 GCTCAGC GC^T[ACGT]AGC
DraIII 398 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 267 TGGCCG [CT]^GGCC[GA]
Eco81I 3720 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2155 GGTAACC G^GT[ACGT]ACC
FbaI 742 TGATCA T^GATCA
FspI 471 TGCGCA TGC^GCA
HindIII 1305 AAGCTT A^AGCTT
HpaI 4280 GTTAAC GTT^AAC
MamI 3434 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 3359 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3209 CACGTG CAC^GTG
PmlI 3209 CACGTG CAC^GTG
SacI 3111 GAGCTC GAGCT^C
SapI 1598 GAAGAGC GCTCTTC(1/4)
SbfI 2606 CCTGCAGG CCTGCA^GG
ScaI 3034 AGTACT AGT^ACT
SexAI 399 ACCAGGT A^CC[AT]GGT
Sse8387I 2606 CCTGCAGG CCTGCA^GG
SspBI 3205 TGTACA T^GTACA
StuI 1360 AGGCCT AGG^CCT
Tth111I 3359 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 183 CCACCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2639 GTAGAC GT^[AC][GT]AC
2924 GTCTAC
AccII 284 CGCG CG^CG
382 CGCG
AflIII 2089 ACATGT A^C[GA][CT]GT
3208 ACACGT
AlwNI 1620 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2625 CAGAATCTG
Asp700I 1766 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2834 GAAAATGTTC
AvaII 114 GGTCC G^G[AT]CC
388 GGACC
BglII 1252 AGATCT A^GATCT
2617 AGATCT
BsaI 860 GAGACC GGTCTC(1/5)
3496 GGTCTC
BspMI 814 ACCTGC ACCTGC(4/8)
130 GCAGGT
BsrFI 147 GCCGGC [GA]^CCGG[CT]
269 GCCGGC
BssSI 1800 CACGAG CACGAG(-5/-1)
2589 CACGAG
BstUI 284 CGCG CG^CG
382 CGCG
Cfr10I 147 GCCGGC [GA]^CCGG[CT]
269 GCCGGC
EarI 4188 CTCTTC CTCTTC(1/4)
1598 GAAGAG
EcoT22I 657 ATGCAT ATGCA^T
3012 ATGCAT
HincII 900 GTCAAC GT[CT]^[GA]AC
4280 GTTAAC
HindII 900 GTCAAC GT[CT]^[GA]AC
4280 GTTAAC
Ksp632I 4188 CTCTTC CTCTTC(1/4)
1598 GAAGAG
MvnI 284 CGCG CG^CG
382 CGCG
NaeI 147 GCCGGC GCC^GGC
269 GCCGGC
NgoMIV 147 GCCGGC G^CCGGC
269 GCCGGC
NsiI 657 ATGCAT ATGCA^T
3012 ATGCAT
PstI 2607 CTGCAG CTGCA^G
2730 CTGCAG
PvuII 1350 CAGCTG CAG^CTG
2889 CAGCTG
SmaI 75 CCCGGG CCC^GGG
158 CCCGGG
XmaCI 75 CCCGGG C^CCGGG
158 CCCGGG
XmaI 75 CCCGGG C^CCGGG
158 CCCGGG
XmnI 1766 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2834 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII