##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
AspEI       AviII       BbeI        BbvCI       BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BsrGI       BssHII      BstBI       BstUI       
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRV       FseI        FspI        
HindIII     HpaI        KasI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NarI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SbfI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       2436  GGTACC               G^GTACC
Alw44I       4896  GTGCAC               G^TGCAC
AocI         3402  CCTGAGG              CC^T[ACGT]AGG
ApaI         1292  GGGCCC               GGGCC^C
ApaLI        4896  GTGCAC               G^TGCAC
Asp718I      2436  GGTACC               G^GTACC
AspI         5759  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcgI         3387  CGATTACTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         5964  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     5964  GCTCAGC              GC^T[ACGT]AGC
BsaBI        2291  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2439  ACCGGA               [AT]^CCGG[AT]
BspLU11I     5672  ACATGT               A^CATGT
BsrBI        5421  GAGCGG               CCGCTC(-3/-3)
BssSI        2591  CTCGTG               CACGAG(-5/-1)
Bst1107I     2339  GTATAC               GTA^TAC
BstEII       2860  GGTCACC              G^GT[ACGT]ACC
BstPI        2860  GGTCACC              G^GT[ACGT]ACC
BstZ17I      2339  GTATAC               GTA^TAC
Bsu36I       3402  CCTGAGG              CC^T[ACGT]AGG
CelII        5964  GCTCAGC              GC^T[ACGT]AGC
DraI         4972  TTTAAA               TTT^AAA
DrdI         1507  GACACGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3402  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      2860  GGTCACC              G^GT[ACGT]ACC
EcoRI        4367  GAATTC               G^AATTC
EcoT22I      1418  ATGCAT               ATGCA^T
HincII         29  GTCGAC               GT[CT]^[GA]AC
HindII         29  GTCGAC               GT[CT]^[GA]AC
KpnI         2436  GGTACC               GGTAC^C
MamI         2291  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         4605  CCATGG               C^CATGG
NdeI         1376  CATATG               CA^TATG
NsiI         1418  ATGCAT               ATGCA^T
PflFI        5759  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SalI           29  GTCGAC               G^TCGAC
SexAI        4030  ACCAGGT              A^CC[AT]GGT
SphI         3207  GCATGC               GCATG^C
SspI         3046  AATATT               AAT^ATT
StuI         1109  AGGCCT               AGG^CCT
Tth111I      5759  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI           29  GTCGAC               GT^[AC][GT]AC
             2339  GTATAC              
AcyI         2501  GGCGTC               G[GA]^CG[CT]C
             6241  GGCGTC              
AflII         931  CTTAAG               C^TTAAG
             3886  CTTAAG              
AvrII        4738  CCTAGG               C^CTAGG
             5538  CCTAGG              
BamHI        1975  GGATCC               G^GATCC
             5534  GGATCC              
BbsI          333  GTCTTC               GAAGAC(2/6)
             5846  GTCTTC              
BciVI        5388  GGATAC               GTATCC(6/5)
             3370  GTATCC              
BfrI          931  CTTAAG               C^TTAAG
             3886  CTTAAG              
BglII        3092  AGATCT               A^GATCT
             3908  AGATCT              
BlnI         4738  CCTAGG               C^CTAGG
             5538  CCTAGG              
BpuAI         333  GTCTTC               GAAGAC(2/6)
             5846  GTCTTC              
BsaHI        2501  GGCGTC               G[GA]^CG[CT]C
             6241  GGCGTC              
BsaI         2278  GAGACC               GGTCTC(1/5)
             3168  GGTCTC              
BsmI         3493  GAATGC               GAATGC(1/-1)
               65  GCATTC              
BsrFI        2525  GCCGGC               [GA]^CCGG[CT]
             2986  GCCGGC              
BstXI        1917  CCACTTCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4709  CCAGTGGAGTGG        
Cfr10I       2525  GCCGGC               [GA]^CCGG[CT]
             2986  GCCGGC              
DraIII        382  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1830  CACCATGTG           
DsaI          178  CCACGG               C^C[GA][CT]GG
             4605  CCATGG              
Hin1I        2501  GGCGTC               G[GA]^CG[CT]C
             6241  GGCGTC              
NaeI         2525  GCCGGC               GCC^GGC
             2986  GCCGGC              
NgoMIV       2525  GCCGGC               G^CCGGC
             2986  GCCGGC              
PshAI        1935  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             6099  GACATCTGTC          
SapI          581  GCTCTTC              GCTCTTC(1/4)
             3574  GCTCTTC             
ScaI         3146  AGTACT               AGT^ACT
             3716  AGTACT              
XcmI         2549  CCACTAAGAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2906  CCAACCCAGAGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbrPI       BbvI        BclI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspHI       
BspMI       BsrDI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PmaCI       PmlI        PpuMI       
PstI        PvuII       RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XbaI        XhoII       XmaCI       
XmaI        XmnI