##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BcgI BclI BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrFI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FbaI FseI FspI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmeI PvuI RsrII SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 9 CGCG CG^CG
AclI 5515 AACGTT AA^CGTT
AcyI 1173 GACGCC G[GA]^CG[CT]C
Alw44I 5615 GTGCAC G^TGCAC
AocI 4623 CCTCAGG CC^T[ACGT]AGG
ApaLI 5615 GTGCAC G^TGCAC
Asp700I 2671 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 4395 TGGCCA TGG^CCA
BamHI 4953 GGATCC G^GATCC
BbrPI 5927 CACGTG CAC^GTG
BbvCI 2524 CCTCAGC CCTCAGC(-5/-2)
BcnI 3502 CCCGG CC^[GC]GG
BglII 3337 AGATCT A^GATCT
BsaHI 1173 GACGCC G[GA]^CG[CT]C
BsaI 4670 GGTCTC GGTCTC(1/5)
BspHI 162 TCATGA T^CATGA
BsrBI 4295 CCGCTC CCGCTC(-3/-3)
BsrDI 1323 CATTGC GCAATG(2/0)
BssSI 4986 CTCGTG CACGAG(-5/-1)
BstEII 1545 GGTTACC G^GT[ACGT]ACC
BstPI 1545 GGTTACC G^GT[ACGT]ACC
BstUI 9 CGCG CG^CG
Bsu36I 4623 CCTCAGG CC^T[ACGT]AGG
DraIII 2895 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 4395 TGGCCA [CT]^GGCC[GA]
Eco81I 4623 CCTCAGG CC^T[ACGT]AGG
EcoNI 5985 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1545 GGTTACC G^GT[ACGT]ACC
EcoRV 788 GATATC GAT^ATC
FauI 4854 CCCGC CCCGC(4/6)
HaeII 3412 GGCGCT [GA]GCGC^[CT]
HapII 3503 CCGG C^CGG
Hin1I 1173 GACGCC G[GA]^CG[CT]C
HpaII 3503 CCGG C^CGG
MfeI 2696 CAATTG C^AATTG
MluNI 4395 TGGCCA TGG^CCA
MscI 4395 TGGCCA TGG^CCA
MspI 3503 CCGG C^CGG
MunI 2696 CAATTG C^AATTG
MvnI 9 CGCG CG^CG
NciI 3502 CCCGG CC^[GC]GG
NcoI 3207 CCATGG C^CATGG
NdeI 3045 CATATG CA^TATG
PacI 3153 TTAATTAA TTAAT^TAA
PmaCI 5927 CACGTG CAC^GTG
PmlI 5927 CACGTG CAC^GTG
PpuMI 1826 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 5515 AACGTT AA^CGTT
PvuII 4161 CAGCTG CAG^CTG
RcaI 162 TCATGA T^CATGA
SacI 5493 GAGCTC GAGCT^C
SapI 3738 GAAGAGC GCTCTTC(1/4)
ScaI 4490 AGTACT AGT^ACT
XmnI 2671 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AseI 290 ATTAAT AT^TAAT
2299 ATTAAT
AsnI 290 ATTAAT AT^TAAT
2299 ATTAAT
BanII 3363 GAGCCC G[GA]GC[CT]^C
5493 GAGCTC
BglI 1122 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5704 GCCTGCATGGC
BlpI 4381 GCTCAGC GC^T[ACGT]AGC
5197 GCTGAGC
Bpu1102I 4381 GCTCAGC GC^T[ACGT]AGC
5197 GCTGAGC
BsaAI 2601 TACGTG [CT]AC^GT[GA]
5927 CACGTG
BstXI 4002 CCACTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5498 CCATTCTTTTGG
BstYI 3337 AGATCT [GA]^GATC[CT]
4953 GGATCC
CelII 4381 GCTCAGC GC^T[ACGT]AGC
5197 GCTGAGC
CfoI 3413 GCGC GCG^C
6019 GCGC
DraII 1826 GGGACCT [GA]G^G[ACGT]CC[CT]
4711 AGGCCCT
DsaI 3207 CCATGG C^C[GA][CT]GG
5427 CCACGG
EcoO109I 1826 GGGACCT [GA]G^G[ACGT]CC[CT]
4711 AGGCCCT
EcoT22I 4939 ATGCAT ATGCA^T
6067 ATGCAT
HgaI 1173 GACGC GACGC(5/10)
254 GCGTC
HhaI 3413 GCGC GCG^C
6019 GCGC
HinP1I 3413 GCGC G^CGC
6019 GCGC
HindIII 1182 AAGCTT A^AGCTT
4375 AAGCTT
MflI 3337 AGATCT [GA]^GATC[CT]
4953 GGATCC
MspA1I 908 CAGCGG C[AC]G^C[GT]G
4161 CAGCTG
NsiI 4939 ATGCAT ATGCA^T
6067 ATGCAT
PshAI 334 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1828 GACCTCTGTC
PshBI 290 ATTAAT AT^TAAT
2299 ATTAAT
SbfI 3406 CCTGCAGG CCTGCA^GG
4542 CCTGCAGG
SpeI 1360 ACTAGT A^CTAGT
3960 ACTAGT
Sse8387I 3406 CCTGCAGG CCTGCA^GG
4542 CCTGCAGG
XhoII 3337 AGATCT [GA]^GATC[CT]
4953 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BbsI BbvI
BciVI BfaI BfrI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BspMI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HpaI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI