##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

Acc65I      AgeI        AscI        AseI        AsnI        
Asp718I     AviII       AvrII       BamHI       BbrPI       
BbvCI       BciVI       BlnI        BsiWI       BspDI       
BssHII      BstBI       ClaI        CpoI        DraIII      
EcoRV       FseI        FspI        HincII      HindII      
HpaI        KpnI        KspI        MfeI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       RsrII       SacII       SalI        
SexAI       SfuI        SgrAI       SnaBI       SphI        
XcmI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        5340  GACGTC               GACGT^C
AccIII       5109  TCCGGA               T^CCGGA
AclI         1731  AACGTT               AA^CGTT
AhdI          864  GACAACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         4916  CCTGAGG              CC^T[ACGT]AGG
Asp700I      5092  GAAATGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         864  GACAACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1452  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         2319  GGCGCC               GGCGC^C
BcgI          998  CGACAACAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3971  TGATCA               T^GATCA
BsaAI        3341  CACGTA               [CT]AC^GT[GA]
BsaBI         598  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        5109  TCCGGA               T^CCGGA
BsmBI        2383  GAGACG               CGTCTC(1/5)
Bsp1407I     5652  TGTACA               T^GTACA
BspEI        5109  TCCGGA               T^CCGGA
BspLU11I     3699  ACATGT               A^CATGT
BsrFI        1737  ACCGGC               [GA]^CCGG[CT]
BsrGI        5652  TGTACA               T^GTACA
Bsu36I       4916  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1737  ACCGGC               [GA]^CCGG[CT]
DrdI         3222  GACGTAGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1804  CGGCCG               C^GGCCG
Eam1105I      864  GACAACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1804  CGGCCG               C^GGCCG
Eco52I       1804  CGGCCG               C^GGCCG
Eco81I       4916  CCTGAGG              CC^T[ACGT]AGG
FbaI         3971  TGATCA               T^GATCA
KasI         2319  GGCGCC               G^GCGCC
MamI          598  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         3913  ACGCGT               A^CGCGT
MroI         5109  TCCGGA               T^CCGGA
NarI         2319  GGCGCC               GG^CGCC
PflFI        1452  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        5500  GACTCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1731  AACGTT               AA^CGTT
PvuI          502  CGATCG               CGAT^CG
SapI         2700  GAAGAGC              GCTCTTC(1/4)
SbfI         1611  CCTGCAGG             CCTGCA^GG
SfiI         2180  GGCCCGGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         2319  GGCGCC               GGC^GCC
SpeI         5676  ACTAGT               A^CTAGT
Sse8387I     1611  CCTGCAGG             CCTGCA^GG
SspBI        5652  TGTACA               T^GTACA
Tth111I      1452  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         6297  TCTAGA               T^CTAGA
XmnI         5092  GAAATGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

ApaI           19  GGGCCC               GGGCC^C
             2093  GGGCCC              
BglII         603  AGATCT               A^GATCT
             1375  AGATCT              
BlpI          732  GCTGAGC              GC^T[ACGT]AGC
             4190  GCTTAGC             
Bpu1102I      732  GCTGAGC              GC^T[ACGT]AGC
             4190  GCTTAGC             
BsaWI         585  TCCGGT               [AT]^CCGG[AT]
             5109  TCCGGA              
BspHI         439  TCATGA               T^CATGA
             5161  TCATGA              
BssSI        5254  CACGAG               CACGAG(-5/-1)
               78  CTCGTG              
BstEII       1658  GGTGACC              G^GT[ACGT]ACC
             4974  GGTCACC             
BstPI        1658  GGTGACC              G^GT[ACGT]ACC
             4974  GGTCACC             
CelII         732  GCTGAGC              GC^T[ACGT]AGC
             4190  GCTTAGC             
EcoO65I      1658  GGTGACC              G^GT[ACGT]ACC
             4974  GGTCACC             
EcoRI         632  GAATTC               G^AATTC
             4855  GAATTC              
EcoT22I      5482  ATGCAT               ATGCA^T
             5923  ATGCAT              
NcoI         2176  CCATGG               C^CATGG
             2378  CCATGG              
NdeI          162  CATATG               CA^TATG
             5842  CATATG              
NsiI         5482  ATGCAT               ATGCA^T
             5923  ATGCAT              
PaeR7I       3806  CTCGAG               C^TCGAG
             4689  CTCGAG              
PflMI         212  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3795  CCAAACACTGG         
PvuII         192  CAGCTG               CAG^CTG
             2469  CAGCTG              
RcaI          439  TCATGA               T^CATGA
             5161  TCATGA              
ScaI         5171  AGTACT               AGT^ACT
             5782  AGTACT              
SmaI          328  CCCGGG               CCC^GGG
             2182  CCCGGG              
SwaI         4283  ATTTAAAT             ATTT^AAAT
             5952  ATTTAAAT            
Van91I        212  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3795  CCAAACACTGG         
XhoI         3806  CTCGAG               C^TCGAG
             4689  CTCGAG              
XmaCI         328  CCCGGG               C^CCGGG
             2182  CCCGGG              
XmaI          328  CCCGGG               C^CCGGG
             2182  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflII       AflIII      AluI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaLI       ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BfrI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrI        BssKI       Bst1107I    
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       BstZ17I     Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        Eco47III    EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII