##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AccIII AclI AflII
Aor51HI AscI AseI AsnI AviII
BbeI BciVI BclI BfrI BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BssHII BssSI Bst1107I BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
FspI HpaI KasI KspI MamI
MfeI MluI MroI MunI NarI
NheI NotI NruI NsiI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SapI ScaI SfiI
SfoI SgrAI SnaBI SpeI SspI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 6207 CGCG CG^CG
AcyI 4909 GGCGTC G[GA]^CG[CT]C
AgeI 1056 ACCGGT A^CCGGT
AhdI 5893 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5893 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1526 CCTAGG C^CTAGG
BcgI 4429 CGAAGCTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1595 AGATCT A^GATCT
BlnI 1526 CCTAGG C^CTAGG
BsaHI 4909 GGCGTC G[GA]^CG[CT]C
BsmI 79 GAATGC GAATGC(1/-1)
Bsp1407I 4888 TGTACA T^GTACA
BspHI 3891 TCATGA T^CATGA
BsrBI 940 CCGCTC CCGCTC(-3/-3)
BsrGI 4888 TGTACA T^GTACA
BstBI 702 TTCGAA TT^CGAA
BstUI 6207 CGCG CG^CG
DraI 2313 TTTAAA TTT^AAA
Eam1105I 5893 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 4909 GGCGTC G[GA]^CG[CT]C
HincII 1013 GTCAAC GT[CT]^[GA]AC
HindII 1013 GTCAAC GT[CT]^[GA]AC
MvnI 6207 CGCG CG^CG
NaeI 371 GCCGGC GCC^GGC
NgoMIV 371 GCCGGC G^CCGGC
NspV 702 TTCGAA TT^CGAA
PacI 2293 TTAATTAA TTAAT^TAA
PinAI 1056 ACCGGT A^CCGGT
PshAI 2929 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3891 TCATGA T^CATGA
SacI 3211 GAGCTC GAGCT^C
SexAI 3752 ACCTGGT A^CC[AT]GGT
SfuI 702 TTCGAA TT^CGAA
SspBI 4888 TGTACA T^GTACA
SwaI 2312 ATTTAAAT ATTT^AAAT
XbaI 4358 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 1059 GGTACC G^GTACC
1465 GGTACC
Alw44I 26 GTGCAC G^TGCAC
2224 GTGCAC
ApaLI 26 GTGCAC G^TGCAC
2224 GTGCAC
Asp700I 1798 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2158 GAACTGTTTC
Asp718I 1059 GGTACC G^GTACC
1465 GGTACC
AspI 826 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3567 GACGGGGTC
BbrPI 1928 CACGTG CAC^GTG
5484 CACGTG
BsaAI 1928 CACGTG [CT]AC^GT[GA]
5484 CACGTG
BstAPI 2964 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4031 GCACCACCTGC
HaeII 4053 AGCGCC [GA]GCGC^[CT]
4782 GGCGCT
HindIII 800 AAGCTT A^AGCTT
4431 AAGCTT
KpnI 1059 GGTACC GGTAC^C
1465 GGTACC
NcoI 2959 CCATGG C^CATGG
3485 CCATGG
NdeI 309 CATATG CA^TATG
3735 CATATG
PflFI 826 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3567 GACGGGGTC
PflMI 2635 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3961 CCAGAACTTGG
PmaCI 1928 CACGTG CAC^GTG
5484 CACGTG
PmlI 1928 CACGTG CAC^GTG
5484 CACGTG
SphI 4630 GCATGC GCATG^C
6121 GCATGC
Tth111I 826 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3567 GACGGGGTC
Van91I 2635 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3961 CCAGAACTTGG
XmnI 1798 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2158 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmaI SmlI Sse8387I
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI