##################################
# NO CUT ENZYMES (   50 enzymes) #
##################################

AccIII      AflII       AgeI        AscI        AseI        
AsnI        BciVI       BfrI        BseAI       BsiWI       
BspDI       BspEI       BspHI       BspLU11I    Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
EcoT22I     HpaI        MfeI        MluI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NsiI        NspV        PacI        PinAI       PmeI        
PshBI       PvuI        RcaI        RsrII       SalI        
SbfI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tru9I       


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AclI         2559  AACGTT               AA^CGTT
AcsI         3764  GAATTC               [GA]^AATT[CT]
AflIII       3144  ACGTGT               A^C[GA][CT]GT
AhdI         4598  GACCTGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3414  AGCGCT               AGC^GCT
ApoI         3764  GAATTC               [GA]^AATT[CT]
AspEI        4598  GACCTGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        4179  TGCGCA               TGC^GCA
AvrII        5880  CCTAGG               C^CTAGG
BbeI         4942  GGCGCC               GGCGC^C
BbvCI        5610  GCTGAGG              CCTCAGC(-5/-2)
BclI         3900  TGATCA               T^GATCA
BlnI         5880  CCTAGG               C^CTAGG
BsaBI        4222  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          163  GAATGC               GAATGC(1/-1)
Bsp1407I     4272  TGTACA               T^GTACA
BsrGI        4272  TGTACA               T^GTACA
BssHII        376  GCGCGC               G^CGCGC
Eam1105I     4598  GACCTGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3414  AGCGCT               AGC^GCT
EcoRI        3764  GAATTC               G^AATTC
EcoRV        1052  GATATC               GAT^ATC
FbaI         3900  TGATCA               T^GATCA
FseI         2530  GGCCGGCC             GGCCGG^CC
FspI         4179  TGCGCA               TGC^GCA
HindIII      2986  AAGCTT               A^AGCTT
KasI         4942  GGCGCC               G^GCGCC
MamI         4222  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         4942  GGCGCC               GG^CGCC
NruI         3547  TCGCGA               TCG^CGA
PaeR7I       5791  CTCGAG               C^TCGAG
Psp1406I     2559  AACGTT               AA^CGTT
SfiI         1519  GGCCGAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         4942  GGCGCC               GGC^GCC
SspBI        4272  TGTACA               T^GTACA
XbaI         5679  TCTAGA               T^CTAGA
XhoI         5791  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AatII        2636  GACGTC               GACGT^C
             4603  GACGTC              
Acc65I       5559  GGTACC               G^GTACC
             5568  GGTACC              
AccI         3659  GTCTAC               GT^[AC][GT]AC
             4052  GTCTAC              
Asp700I       430  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3760  GAAGGAATTC          
Asp718I      5559  GGTACC               G^GTACC
             5568  GGTACC              
AspI          290  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3854  GACCGCGTC           
BbrPI        1775  CACGTG               CAC^GTG
             2111  CACGTG              
BcgI         3375  CGATGACGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2247  GCAAGAATATCG        
BglII        4866  AGATCT               A^GATCT
             5241  AGATCT              
BlpI         3601  GCTCAGC              GC^T[ACGT]AGC
             5197  GCTGAGC             
Bpu1102I     3601  GCTCAGC              GC^T[ACGT]AGC
             5197  GCTGAGC             
BsaWI         576  ACCGGA               [AT]^CCGG[AT]
             5067  TCCGGT              
CelII        3601  GCTCAGC              GC^T[ACGT]AGC
             5197  GCTGAGC             
KpnI         5559  GGTACC               GGTAC^C
             5568  GGTACC              
NcoI         3054  CCATGG               C^CATGG
             3633  CCATGG              
NspI          753  GCATGT               [GA]CATG^[CT]
             5116  GCATGT              
PflFI         290  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3854  GACCGCGTC           
PmaCI        1775  CACGTG               CAC^GTG
             2111  CACGTG              
PmlI         1775  CACGTG               CAC^GTG
             2111  CACGTG              
PshAI        2000  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             6079  GACGTAGGTC          
SacI          481  GAGCTC               GAGCT^C
             4133  GAGCTC              
Tth111I       290  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3854  GACCGCGTC           
XmnI          430  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3760  GAAGGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  148 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BamHI       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BssSI       BstAPI      BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DrdI        DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacII       SapI        
Sau3AI      Sau96I      ScaI        ScrFI       SexAI       
SfaNI       SfcI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI