##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AgeI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp718I     AspI        BciVI       BclI        
BfrI        BglII       BsiWI       BspDI       BssHII      
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        EagI        EclXI       Eco47III    Eco52I      
EcoRI       FbaI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MluI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        ScaI        
SfuI        SgrAI       SnaBI       SpeI        SspI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII         778  GACGTC               GACGT^C
AccII        5911  CGCG                 CG^CG
AhdI          293  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      5391  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         293  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        5489  TGCGCA               TGC^GCA
AvrII         691  CCTAGG               C^CTAGG
BamHI        1332  GGATCC               G^GATCC
BlnI          691  CCTAGG               C^CTAGG
BlpI         4892  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4892  GCTCAGC              GC^T[ACGT]AGC
BsaBI        4820  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        5073  CGGTCG               CG[GA][CT]^CG
BsmBI        4447  CGTCTC               CGTCTC(1/5)
Bsp1407I     4870  TGTACA               T^GTACA
BsrGI        4870  TGTACA               T^GTACA
BstUI        5911  CGCG                 CG^CG
CelII        4892  GCTCAGC              GC^T[ACGT]AGC
DraIII       3665  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         5066  GACACCTCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      293  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1185  GATATC               GAT^ATC
EcoT22I      2753  ATGCAT               ATGCA^T
FspI         5489  TGCGCA               TGC^GCA
MamI         4820  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         4556  CAATTG               C^AATTG
MunI         4556  CAATTG               C^AATTG
MvnI         5911  CGCG                 CG^CG
NsiI         2753  ATGCAT               ATGCA^T
PaeR7I       3678  CTCGAG               C^TCGAG
PshAI           2  GACCTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         3431  GAGCTC               GAGCT^C
SapI         5316  GAAGAGC              GCTCTTC(1/4)
SbfI         5344  CCTGCAGG             CCTGCA^GG
SexAI        6052  ACCTGGT              A^CC[AT]GGT
Sse8387I     5344  CCTGCAGG             CCTGCA^GG
SspBI        4870  TGTACA               T^GTACA
XhoI         3678  CTCGAG               C^TCGAG
XmnI         5391  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccIII       1634  TCCGGA               T^CCGGA
             6027  TCCGGA              
BbeI         1469  GGCGCC               GGCGC^C
             4925  GGCGCC              
BcgI           44  CGAGGGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5689  GCAGGGTGGTCG        
BsaWI        1634  TCCGGA               [AT]^CCGG[AT]
             6027  TCCGGA              
BseAI        1634  TCCGGA               T^CCGGA
             6027  TCCGGA              
BspEI        1634  TCCGGA               T^CCGGA
             6027  TCCGGA              
BspLU11I      772  ACATGT               A^CATGT
             3007  ACATGT              
BsrBI        4058  CCGCTC               CCGCTC(-3/-3)
             4664  CCGCTC              
BsrDI        1076  GCAATG               GCAATG(2/0)
             1851  GCAATG              
FseI         2007  GGCCGGCC             GGCCGG^CC
             3186  GGCCGGCC            
KasI         1469  GGCGCC               G^GCGCC
             4925  GGCGCC              
MroI         1634  TCCGGA               T^CCGGA
             6027  TCCGGA              
NarI         1469  GGCGCC               GG^CGCC
             4925  GGCGCC              
SfiI          699  GGCCCAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             5760  GGCCAGAGAGGCC       
SfoI         1469  GGCGCC               GGC^GCC
             4925  GGCGCC              
SmaI         1423  CCCGGG               CCC^GGG
             2688  CCCGGG              
SphI         4029  GCATGC               GCATG^C
             4943  GCATGC              
XcmI          165  CCATAGAGACCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3334  CCAGTGGCTTTCTGG     
XmaCI        1423  CCCGGG               C^CCGGG
             2688  CCCGGG              
XmaI         1423  CCCGGG               C^CCGGG
             2688  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AciI        AcyI        AfaI        AflIII      AluI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbrPI       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspHI       BspMI       BsrFI       BsrI        
BssKI       BssSI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII