##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AccIII Alw44I ApaLI AscI AseI
AsnI BclI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstZ17I ClaI DrdI EcoT22I FbaI
KspI MluI MroI NdeI NheI
NotI NsiI PacI PmeI PshBI
PvuI SacII SalI SbfI SfiI
SnaBI Sse8387I SspBI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 9035 GACGTC GACGT^C
AccI 4374 GTCTAC GT^[AC][GT]AC
AclI 2003 AACGTT AA^CGTT
AflII 5571 CTTAAG C^TTAAG
AflIII 4065 ACATGT A^C[GA][CT]GT
Asp700I 521 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 2991 GGCGCC GGCGC^C
BcgI 8825 CGACCCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5571 CTTAAG C^TTAAG
BsaBI 2743 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 4065 ACATGT A^CATGT
BstBI 7709 TTCGAA TT^CGAA
CpoI 10330 CGGTCCG CG^G[AT]CCG
DraI 2020 TTTAAA TTT^AAA
EagI 4868 CGGCCG C^GGCCG
EclXI 4868 CGGCCG C^GGCCG
Eco52I 4868 CGGCCG C^GGCCG
EcoRI 10767 GAATTC G^AATTC
FseI 6325 GGCCGGCC GGCCGG^CC
HindIII 1849 AAGCTT A^AGCTT
KasI 2991 GGCGCC G^GCGCC
MamI 2743 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5494 CAATTG C^AATTG
MunI 5494 CAATTG C^AATTG
NarI 2991 GGCGCC GG^CGCC
NruI 2343 TCGCGA TCG^CGA
NspV 7709 TTCGAA TT^CGAA
Psp1406I 2003 AACGTT AA^CGTT
RsrII 10330 CGGTCCG CG^G[AT]CCG
SexAI 4029 ACCTGGT A^CC[AT]GGT
SfoI 2991 GGCGCC GGC^GCC
SfuI 7709 TTCGAA TT^CGAA
SgrAI 6604 CACCGGCG C[GA]^CCGG[CT]G
SpeI 7819 ACTAGT A^CTAGT
XmnI 521 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 4410 GGTACC G^GTACC
4641 GGTACC
AccII 2344 CGCG CG^CG
9561 CGCG
AgeI 2673 ACCGGT A^CCGGT
4407 ACCGGT
Aor51HI 2213 AGCGCT AGC^GCT
6407 AGCGCT
Asp718I 4410 GGTACC G^GTACC
4641 GGTACC
BamHI 10507 GGATCC G^GATCC
10557 GGATCC
BciVI 1185 GGATAC GTATCC(6/5)
4981 GTATCC
BglII 7218 AGATCT A^GATCT
10166 AGATCT
BsiEI 2671 CGACCG CG[GA][CT]^CG
4868 CGGCCG
BsmBI 2247 GAGACG CGTCTC(1/5)
8000 GAGACG
BspHI 1011 TCATGA T^CATGA
2603 TCATGA
BsrBI 4958 CCGCTC CCGCTC(-3/-3)
10312 GAGCGG
BstUI 2344 CGCG CG^CG
9561 CGCG
DraIII 5647 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
10006 CACGTGGTG
Eco47III 2213 AGCGCT AGC^GCT
6407 AGCGCT
EcoRV 6226 GATATC GAT^ATC
9772 GATATC
HpaI 3510 GTTAAC GTT^AAC
9227 GTTAAC
KpnI 4410 GGTACC GGTAC^C
4641 GGTACC
MvnI 2344 CGCG CG^CG
9561 CGCG
PinAI 2673 ACCGGT A^CCGGT
4407 ACCGGT
RcaI 1011 TCATGA T^CATGA
2603 TCATGA
ScaI 668 AGTACT AGT^ACT
1598 AGTACT
SphI 4099 GCATGC GCATG^C
5439 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 159 enzymes) #
#########################################
AciI AcsI AcyI AfaI AhdI
AluI AlwI AlwNI AocI ApaI
ApoI AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflFI PflMI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI