##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AseI AsnI Asp718I AspEI
AviII BclI BfrI BsaBI BsiEI
BsiWI BspDI Bst1107I BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoRI EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SbfI ScaI SfiI
SnaBI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 4318 GTCGAC GT^[AC][GT]AC
Alw44I 4443 GTGCAC G^TGCAC
Aor51HI 2964 AGCGCT AGC^GCT
ApaLI 4443 GTGCAC G^TGCAC
AscI 6418 GGCGCGCC GG^CGCGCC
Asp700I 2427 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 2086 GCTGAGG CCTCAGC(-5/-2)
BciVI 2251 GTATCC GTATCC(6/5)
BglII 5272 AGATCT A^GATCT
BsmBI 1942 GAGACG CGTCTC(1/5)
BsmI 4641 GCATTC GAATGC(1/-1)
BspHI 26 TCATGA T^CATGA
BsrDI 5508 CATTGC GCAATG(2/0)
BssHII 6419 GCGCGC G^CGCGC
BssSI 1894 CACGAG CACGAG(-5/-1)
BstBI 3325 TTCGAA TT^CGAA
BstEII 5987 GGTGACC G^GT[ACGT]ACC
BstPI 5987 GGTGACC G^GT[ACGT]ACC
DraI 6157 TTTAAA TTT^AAA
DrdI 4617 GACCTACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2964 AGCGCT AGC^GCT
EcoO65I 5987 GGTGACC G^GT[ACGT]ACC
EcoRV 2929 GATATC GAT^ATC
MfeI 4545 CAATTG C^AATTG
MunI 4545 CAATTG C^AATTG
NspV 3325 TTCGAA TT^CGAA
PaeR7I 2943 CTCGAG C^TCGAG
RcaI 26 TCATGA T^CATGA
SalI 4318 GTCGAC G^TCGAC
SexAI 581 ACCTGGT A^CC[AT]GGT
SfuI 3325 TTCGAA TT^CGAA
SgrAI 2281 CACCGGCG C[GA]^CCGG[CT]G
SpeI 1596 ACTAGT A^CTAGT
SphI 1391 GCATGC GCATG^C
SspI 1184 AATATT AAT^ATT
XhoI 2943 CTCGAG C^TCGAG
XmnI 2427 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 2489 TCCGGA T^CCGGA
3955 TCCGGA
AcsI 2170 AAATTC [GA]^AATT[CT]
5434 AAATTT
ApoI 2170 AAATTC [GA]^AATT[CT]
5434 AAATTT
AspI 409 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5873 GACTTGGTC
AvrII 3434 CCTAGG C^CTAGG
4279 CCTAGG
BamHI 3650 GGATCC G^GATCC
4661 GGATCC
BbeI 2285 GGCGCC GGCGC^C
3422 GGCGCC
BbrPI 1567 CACGTG CAC^GTG
4699 CACGTG
BcgI 5613 CGAAGCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3795 GCACTTGCCTCG
BlnI 3434 CCTAGG C^CTAGG
4279 CCTAGG
BseAI 2489 TCCGGA T^CCGGA
3955 TCCGGA
Bsp1407I 2837 TGTACA T^GTACA
3250 TGTACA
BspEI 2489 TCCGGA T^CCGGA
3955 TCCGGA
BspLU11I 4193 ACATGT A^CATGT
4752 ACATGT
BsrGI 2837 TGTACA T^GTACA
3250 TGTACA
BstAPI 192 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1563 GCAGCACGTGC
KasI 2285 GGCGCC G^GCGCC
3422 GGCGCC
MroI 2489 TCCGGA T^CCGGA
3955 TCCGGA
NarI 2285 GGCGCC GG^CGCC
3422 GGCGCC
NcoI 2537 CCATGG C^CATGG
5515 CCATGG
PflFI 409 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5873 GACTTGGTC
PmaCI 1567 CACGTG CAC^GTG
4699 CACGTG
PmlI 1567 CACGTG CAC^GTG
4699 CACGTG
SacI 927 GAGCTC GAGCT^C
1162 GAGCTC
SapI 1446 GAAGAGC GCTCTTC(1/4)
3737 GCTCTTC
SfoI 2285 GGCGCC GGC^GCC
3422 GGCGCC
SspBI 2837 TGTACA T^GTACA
3250 TGTACA
Tth111I 409 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5873 GACTTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI