##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI ApaI AscI Asp700I
Asp718I AspEI AspI AviII BamHI
BbsI BciVI BclI BfrI BpuAI
BsaBI BsaI BseAI BsiWI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CpoI DrdI Eam1105I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 347 GACGTC GACGT^C
AcsI 2300 GAATTT [GA]^AATT[CT]
Alw44I 1924 GTGCAC G^TGCAC
Aor51HI 113 AGCGCT AGC^GCT
ApaLI 1924 GTGCAC G^TGCAC
ApoI 2300 GAATTT [GA]^AATT[CT]
AvaI 650 CTCGGG C^[CT]CG[GA]G
AvrII 1300 CCTAGG C^CTAGG
BalI 771 TGGCCA TGG^CCA
BbeI 89 GGCGCC GGCGC^C
BbrPI 530 CACGTG CAC^GTG
BcgI 68 GCAGCGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1830 AGATCT A^GATCT
BlnI 1300 CCTAGG C^CTAGG
BlpI 1676 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1676 GCTCAGC GC^T[ACGT]AGC
BsaAI 530 CACGTG [CT]AC^GT[GA]
BsaWI 272 ACCGGA [AT]^CCGG[AT]
BsmBI 871 GAGACG CGTCTC(1/5)
BsoBI 650 CTCGGG C^[CT]CG[GA]G
Bsp1407I 885 TGTACA T^GTACA
BspDI 1349 ATCGAT AT^CGAT
BsrDI 2270 GCAATG GCAATG(2/0)
BsrGI 885 TGTACA T^GTACA
CelII 1676 GCTCAGC GC^T[ACGT]AGC
ClaI 1349 ATCGAT AT^CGAT
DraI 2413 TTTAAA TTT^AAA
Eco47III 113 AGCGCT AGC^GCT
KasI 89 GGCGCC G^GCGCC
KspI 93 CCGCGG CCGC^GG
MluNI 771 TGGCCA TGG^CCA
MscI 771 TGGCCA TGG^CCA
NarI 89 GGCGCC GG^CGCC
PmaCI 530 CACGTG CAC^GTG
PmlI 530 CACGTG CAC^GTG
SacII 93 CCGCGG CCGC^GG
SfiI 97 GGCCCGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 89 GGCGCC GGC^GCC
SmlI 1622 CTCAAG C^T[CT][GA]AG
SspBI 885 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 86 CGCG CG^CG
94 CGCG
AflIII 531 ACGTGT A^C[GA][CT]GT
2161 ACACGT
AseI 1405 ATTAAT AT^TAAT
2284 ATTAAT
AsnI 1405 ATTAAT AT^TAAT
2284 ATTAAT
BbvCI 841 CCTCAGC CCTCAGC(-5/-2)
1782 CCTCAGC
BpmI 1062 CTCCAG CTGGAG(16/14)
2185 CTCCAG
BsgI 377 CTGCAC GTGCAG(16/14)
2083 GTGCAG
BsiEI 32 CGGCCG CG[GA][CT]^CG
50 CGGCCG
BsmI 1341 GAATGC GAATGC(1/-1)
1809 GCATTC
BspMI 1437 ACCTGC ACCTGC(4/8)
2199 ACCTGC
BsrFI 34 GCCGGC [GA]^CCGG[CT]
938 GCCGGC
BstUI 86 CGCG CG^CG
94 CGCG
CfoI 90 GCGC GCG^C
114 GCGC
Cfr10I 34 GCCGGC [GA]^CCGG[CT]
938 GCCGGC
EagI 32 CGGCCG C^GGCCG
50 CGGCCG
EclXI 32 CGGCCG C^GGCCG
50 CGGCCG
Eco52I 32 CGGCCG C^GGCCG
50 CGGCCG
HaeII 89 GGCGCC [GA]GCGC^[CT]
113 AGCGCT
HhaI 90 GCGC GCG^C
114 GCGC
HinP1I 90 GCGC G^CGC
114 GCGC
MslI 1402 CACATTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1772 CACCAATGTG
MvnI 86 CGCG CG^CG
94 CGCG
NaeI 34 GCCGGC GCC^GGC
938 GCCGGC
NgoMIV 34 GCCGGC G^CCGGC
938 GCCGGC
NspI 580 GCATGT [GA]CATG^[CT]
2153 ACATGC
PflMI 1390 CCACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2140 CCATCAGCTGG
PshAI 347 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
577 GACGCATGTC
PshBI 1405 ATTAAT AT^TAAT
2284 ATTAAT
PvuII 992 CAGCTG CAG^CTG
2144 CAGCTG
SacI 266 GAGCTC GAGCT^C
302 GAGCTC
Van91I 1390 CCACTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2140 CCATCAGCTGG
XcmI 1116 CCAAAGGGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1837 CCACCACTCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AvaII BanI
BanII BbvI BcnI BfaI BglI
BmyI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HgaI Hin1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII