##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII BsaBI BsiEI BsiWI BsmBI
BspDI BsrBI BssHII BstBI BstUI
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoRI FseI FspI HpaI
KpnI KspI MamI MluI MvnI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SfiI SfuI SgrAI SnaBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflII 1785 CTTAAG C^TTAAG
AhdI 4137 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1873 GTGCAC G^TGCAC
ApaLI 1873 GTGCAC G^TGCAC
Asp700I 2415 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4137 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2638 CCTAGG C^CTAGG
BamHI 2236 GGATCC G^GATCC
BbeI 4745 GGCGCC GGCGC^C
BbrPI 1361 CACGTG CAC^GTG
BcgI 6952 GCATGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 6978 GGATAC GTATCC(6/5)
BfrI 1785 CTTAAG C^TTAAG
BlnI 2638 CCTAGG C^CTAGG
BsaAI 1361 CACGTG [CT]AC^GT[GA]
Bsp1407I 2024 TGTACA T^GTACA
BsrGI 2024 TGTACA T^GTACA
BstEII 6847 GGTGACC G^GT[ACGT]ACC
BstPI 6847 GGTGACC G^GT[ACGT]ACC
DraIII 1014 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4137 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 6847 GGTGACC G^GT[ACGT]ACC
EcoRV 64 GATATC GAT^ATC
HaeII 4745 GGCGCC [GA]GCGC^[CT]
HindIII 7736 AAGCTT A^AGCTT
KasI 4745 GGCGCC G^GCGCC
MfeI 7696 CAATTG C^AATTG
MunI 7696 CAATTG C^AATTG
NaeI 3801 GCCGGC GCC^GGC
NarI 4745 GGCGCC GG^CGCC
NgoMIV 3801 GCCGGC G^CCGGC
NheI 969 GCTAGC G^CTAGC
PaeR7I 6926 CTCGAG C^TCGAG
PmaCI 1361 CACGTG CAC^GTG
PmlI 1361 CACGTG CAC^GTG
PshAI 2861 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 5720 GCTCTTC GCTCTTC(1/4)
SbfI 3759 CCTGCAGG CCTGCA^GG
ScaI 408 AGTACT AGT^ACT
SfoI 4745 GGCGCC GGC^GCC
SmaI 310 CCCGGG CCC^GGG
Sse8387I 3759 CCTGCAGG CCTGCA^GG
SspBI 2024 TGTACA T^GTACA
XhoI 6926 CTCGAG C^TCGAG
XmaCI 310 CCCGGG C^CCGGG
XmaI 310 CCCGGG C^CCGGG
XmnI 2415 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 1301 TCCGGA T^CCGGA
5214 TCCGGA
AcyI 3846 GGCGTC G[GA]^CG[CT]C
4745 GGCGCC
AspI 2689 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4645 GACAAGGTC
BbsI 2100 GAAGAC GAAGAC(2/6)
6658 GTCTTC
BpuAI 2100 GAAGAC GAAGAC(2/6)
6658 GTCTTC
BsaHI 3846 GGCGTC G[GA]^CG[CT]C
4745 GGCGCC
BsaWI 1301 TCCGGA [AT]^CCGG[AT]
5214 TCCGGA
BseAI 1301 TCCGGA T^CCGGA
5214 TCCGGA
BspEI 1301 TCCGGA T^CCGGA
5214 TCCGGA
BsrDI 7031 CATTGC GCAATG(2/0)
7108 CATTGC
BsrFI 1907 GCCGGT [GA]^CCGG[CT]
3801 GCCGGC
BssSI 2179 CACGAG CACGAG(-5/-1)
3529 CACGAG
Bst1107I 1356 GTATAC GTA^TAC
5969 GTATAC
BstZ17I 1356 GTATAC GTA^TAC
5969 GTATAC
CfoI 4746 GCGC GCG^C
5281 GCGC
Cfr10I 1907 GCCGGT [GA]^CCGG[CT]
3801 GCCGGC
DrdI 1280 GACAAGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4554 GACAACCATGTC
EcoT22I 3574 ATGCAT ATGCA^T
6944 ATGCAT
HgaI 2103 GACGC GACGC(5/10)
3847 GCGTC
HhaI 4746 GCGC GCG^C
5281 GCGC
Hin1I 3846 GGCGTC G[GA]^CG[CT]C
4745 GGCGCC
HinP1I 4746 GCGC G^CGC
5281 GCGC
MroI 1301 TCCGGA T^CCGGA
5214 TCCGGA
NsiI 3574 ATGCAT ATGCA^T
6944 ATGCAT
PflFI 2689 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4645 GACAAGGTC
SalI 4583 GTCGAC G^TCGAC
5642 GTCGAC
SpeI 6127 ACTAGT A^CTAGT
7420 ACTAGT
SphI 7874 GCATGC GCATG^C
7970 GCATGC
Tth111I 2689 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4645 GACAAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BclI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspLU11I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII