##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AflII AscI AseI
AsnI Asp718I BbeI BcgI BciVI
BclI BfrI BsaBI BsiWI BspDI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI EcoRI EcoRV FbaI
FseI HindIII HpaI KasI KpnI
MamI MfeI MluI MunI NarI
NheI NotI NruI NspV PacI
PaeR7I PmeI PshBI RsrII SalI
SbfI SfiI SfoI SfuI SnaBI
SpeI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 421 TCCGGA T^CCGGA
AclI 7324 AACGTT AA^CGTT
AcyI 7455 GGCGTC G[GA]^CG[CT]C
AgeI 3793 ACCGGT A^CCGGT
AhdI 3231 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3231 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1568 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4896 TGCGCA TGC^GCA
BlpI 2893 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2893 GCTGAGC GC^T[ACGT]AGC
BsaHI 7455 GGCGTC G[GA]^CG[CT]C
BseAI 421 TCCGGA T^CCGGA
BspEI 421 TCCGGA T^CCGGA
CelII 2893 GCTGAGC GC^T[ACGT]AGC
DraIII 2261 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1931 CGGCCG C^GGCCG
Eam1105I 3231 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1931 CGGCCG C^GGCCG
Eco52I 1931 CGGCCG C^GGCCG
FspI 4896 TGCGCA TGC^GCA
Hin1I 7455 GGCGTC G[GA]^CG[CT]C
MroI 421 TCCGGA T^CCGGA
NdeI 3459 CATATG CA^TATG
PflFI 1568 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3793 ACCGGT A^CCGGT
PshAI 3370 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 7324 AACGTT AA^CGTT
PvuI 34 CGATCG CGAT^CG
SapI 1814 GAAGAGC GCTCTTC(1/4)
ScaI 999 AGTACT AGT^ACT
SgrAI 3792 CACCGGTG C[GA]^CCGG[CT]G
Tth111I 1568 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3364 TCTAGA T^CTAGA
XcmI 5895 CCAAGAACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 886 GTCTAC GT^[AC][GT]AC
4964 GTAGAC
AvrII 4614 CCTAGG C^CTAGG
5292 CCTAGG
BamHI 5804 GGATCC G^GATCC
7369 GGATCC
BbrPI 6750 CACGTG CAC^GTG
7303 CACGTG
BlnI 4614 CCTAGG C^CTAGG
5292 CCTAGG
Bsp1407I 1503 TGTACA T^GTACA
3943 TGTACA
BspHI 1173 TCATGA T^CATGA
7134 TCATGA
BsrGI 1503 TGTACA T^GTACA
3943 TGTACA
DrdI 769 GACCACTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6971 GACAAAACAGTC
HincII 4797 GTTGAC GT[CT]^[GA]AC
7405 GTTGAC
HindII 4797 GTTGAC GT[CT]^[GA]AC
7405 GTTGAC
PmaCI 6750 CACGTG CAC^GTG
7303 CACGTG
PmlI 6750 CACGTG CAC^GTG
7303 CACGTG
RcaI 1173 TCATGA T^CATGA
7134 TCATGA
SacI 4568 GAGCTC GAGCT^C
5059 GAGCTC
SexAI 3011 ACCAGGT A^CC[AT]GGT
7426 ACCTGGT
SspBI 1503 TGTACA T^GTACA
3943 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI XmnI