##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        ApaI        
AscI        AseI        AsnI        Asp718I     AvrII       
BbrPI       BcgI        BciVI       BlnI        BlpI        
Bpu1102I    BsiWI       BsrDI       BssHII      Bst1107I    
BstBI       BstZ17I     CelII       CpoI        DrdI        
EcoRV       FseI        KpnI        KspI        MfeI        
MluI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    RsrII       SacII       
ScaI        SexAI       SfiI        SfuI        SgrAI       
SpeI        StuI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII       3359  TCCGGA               T^CCGGA
AflII        1427  CTTAAG               C^TTAAG
AhdI          110  GACTGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       515  AGCGCT               AGC^GCT
AspEI         110  GACTGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1237  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1005  TGCGCA               TGC^GCA
BbeI         4174  GGCGCC               GGCGC^C
BbvCI         518  GCTGAGG              CCTCAGC(-5/-2)
BfrI         1427  CTTAAG               C^TTAAG
BglII        1265  AGATCT               A^GATCT
BsaAI        1415  TACGTA               [CT]AC^GT[GA]
BseAI        3359  TCCGGA               T^CCGGA
BsmI         5106  GAATGC               GAATGC(1/-1)
Bsp1407I     4399  TGTACA               T^GTACA
BspDI        3928  ATCGAT               AT^CGAT
BspEI        3359  TCCGGA               T^CCGGA
BsrFI        3667  GCCGGT               [GA]^CCGG[CT]
BsrGI        4399  TGTACA               T^GTACA
BstEII       3887  GGTGACC              G^GT[ACGT]ACC
BstPI        3887  GGTGACC              G^GT[ACGT]ACC
Cfr10I       3667  GCCGGT               [GA]^CCGG[CT]
ClaI         3928  ATCGAT               AT^CGAT
DraIII       3343  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      110  GACTGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      515  AGCGCT               AGC^GCT
EcoO65I      3887  GGTGACC              G^GT[ACGT]ACC
EcoRI        2538  GAATTC               G^AATTC
EcoT22I      1647  ATGCAT               ATGCA^T
FspI         1005  TGCGCA               TGC^GCA
HpaI         4713  GTTAAC               GTT^AAC
KasI         4174  GGCGCC               G^GCGCC
MroI         3359  TCCGGA               T^CCGGA
NarI         4174  GGCGCC               GG^CGCC
NcoI         1635  CCATGG               C^CATGG
NsiI         1647  ATGCAT               ATGCA^T
PflFI        1237  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2896  CCATCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI         1762  CGATCG               CGAT^CG
SalI         1397  GTCGAC               G^TCGAC
SbfI         3535  CCTGCAGG             CCTGCA^GG
SfoI         4174  GGCGCC               GGC^GCC
SmaI         2555  CCCGGG               CCC^GGG
SnaBI        1415  TACGTA               TAC^GTA
SphI         2985  GCATGC               GCATG^C
Sse8387I     3535  CCTGCAGG             CCTGCA^GG
SspBI        4399  TGTACA               T^GTACA
SspI         4830  AATATT               AAT^ATT
Tth111I      1237  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2896  CCATCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3997  TCTAGA               T^CTAGA
XmaCI        2555  CCCGGG               C^CCGGG
XmaI         2555  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

Alw44I       4136  GTGCAC               G^TGCAC
             5308  GTGCAC              
AocI         3489  CCTCAGG              CC^T[ACGT]AGG
             3824  CCTCAGG             
ApaLI        4136  GTGCAC               G^TGCAC
             5308  GTGCAC              
BamHI        3266  GGATCC               G^GATCC
             4042  GGATCC              
BclI         1945  TGATCA               T^GATCA
             3294  TGATCA              
BsaWI        3359  TCCGGA               [AT]^CCGG[AT]
             3701  ACCGGA              
BsmBI        2920  CGTCTC               CGTCTC(1/5)
             3201  CGTCTC              
BspLU11I     1703  ACATGT               A^CATGT
             2218  ACATGT              
BstAPI       2976  GCATCCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4756  GCATGATTTGC         
Bsu36I       3489  CCTCAGG              CC^T[ACGT]AGG
             3824  CCTCAGG             
EagI         3051  CGGCCG               C^GGCCG
             4226  CGGCCG              
EclXI        3051  CGGCCG               C^GGCCG
             4226  CGGCCG              
Eco52I       3051  CGGCCG               C^GGCCG
             4226  CGGCCG              
Eco81I       3489  CCTCAGG              CC^T[ACGT]AGG
             3824  CCTCAGG             
FbaI         1945  TGATCA               T^GATCA
             3294  TGATCA              
HindIII      2003  AAGCTT               A^AGCTT
             5534  AAGCTT              
PpuMI         995  AGGTCCT              [GA]G^G[AT]CC[CT]
             3965  GGGTCCC             
SacI          138  GAGCTC               GAGCT^C
             1898  GAGCTC              
SapI          956  GAAGAGC              GCTCTTC(1/4)
             4166  GAAGAGC             


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApoI        Asp700I     AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaBI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspHI       BspMI       BsrBI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmnI