##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AcyI AflII Aor51HI
AscI AviII BbeI BcgI BfrI
BglI BsaHI BsiWI BsmBI BspDI
BspHI Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DrdI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HindIII HpaI KasI KspI
MfeI MluI MunI NaeI NarI
NgoMIV NotI NruI NsiI PacI
PaeR7I PmeI Psp1406I PstI PvuI
RcaI RsrII SacII SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AgeI 3880 ACCGGT A^CCGGT
AhdI 4354 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4749 GTGCAC G^TGCAC
ApaLI 4749 GTGCAC G^TGCAC
AseI 1390 ATTAAT AT^TAAT
AsnI 1390 ATTAAT AT^TAAT
Asp700I 4296 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4354 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2044 CACGTG CAC^GTG
BbsI 3503 GTCTTC GAAGAC(2/6)
BbvCI 3778 GCTGAGG CCTCAGC(-5/-2)
BclI 1474 TGATCA T^GATCA
BpuAI 3503 GTCTTC GAAGAC(2/6)
BsaAI 2044 CACGTG [CT]AC^GT[GA]
BsrBI 4444 CCGCTC CCGCTC(-3/-3)
BsrFI 3880 ACCGGT [GA]^CCGG[CT]
BssSI 4982 CACGAG CACGAG(-5/-1)
BstAPI 3764 GCAGTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4965 TTCGAA TT^CGAA
Cfr10I 3880 ACCGGT [GA]^CCGG[CT]
Eam1105I 4354 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3145 CCTAGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1474 TGATCA T^GATCA
NcoI 2837 CCATGG C^CATGG
NspV 4965 TTCGAA TT^CGAA
PinAI 3880 ACCGGT A^CCGGT
PmaCI 2044 CACGTG CAC^GTG
PmlI 2044 CACGTG CAC^GTG
PshAI 281 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1390 ATTAAT AT^TAAT
SalI 53 GTCGAC G^TCGAC
SexAI 3543 ACCTGGT A^CC[AT]GGT
SfuI 4965 TTCGAA TT^CGAA
SpeI 1905 ACTAGT A^CTAGT
SspI 2129 AATATT AAT^ATT
XbaI 4006 TCTAGA T^CTAGA
XmnI 4296 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 3870 GGTACC G^GTACC
4236 GGTACC
AccIII 74 TCCGGA T^CCGGA
1289 TCCGGA
Asp718I 3870 GGTACC G^GTACC
4236 GGTACC
AspI 4739 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6261 GACTCTGTC
BsaBI 3512 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4419 GATTTGGATC
BseAI 74 TCCGGA T^CCGGA
1289 TCCGGA
BsiEI 5492 CGGCCG CG[GA][CT]^CG
5569 CGGCCG
BspEI 74 TCCGGA T^CCGGA
1289 TCCGGA
BspLU11I 931 ACATGT A^CATGT
1729 ACATGT
BssHII 5419 GCGCGC G^CGCGC
5446 GCGCGC
DraI 6248 TTTAAA TTT^AAA
6297 TTTAAA
DsaI 180 CCACGG C^C[GA][CT]GG
2837 CCATGG
EagI 5492 CGGCCG C^GGCCG
5569 CGGCCG
EclXI 5492 CGGCCG C^GGCCG
5569 CGGCCG
Eco52I 5492 CGGCCG C^GGCCG
5569 CGGCCG
HaeII 139 GGCGCT [GA]GCGC^[CT]
5501 GGCGCT
HgaI 3681 GCGTC GACGC(5/10)
6175 GCGTC
HincII 53 GTCGAC GT[CT]^[GA]AC
518 GTCAAC
HindII 53 GTCGAC GT[CT]^[GA]AC
518 GTCAAC
KpnI 3870 GGTACC GGTAC^C
4236 GGTACC
MamI 3512 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4419 GATTTGGATC
MroI 74 TCCGGA T^CCGGA
1289 TCCGGA
NdeI 1218 CATATG CA^TATG
1811 CATATG
NheI 664 GCTAGC G^CTAGC
4488 GCTAGC
PflFI 4739 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6261 GACTCTGTC
SacI 104 GAGCTC GAGCT^C
4089 GAGCTC
SapI 1944 GCTCTTC GCTCTTC(1/4)
2210 GCTCTTC
SmlI 2387 CTTGAG C^T[CT][GA]AG
2602 CTTGAG
SphI 4018 GCATGC GCATG^C
5347 GCATGC
Tth111I 4739 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6261 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
AvrII BalI BamHI BanI BanII
BbvI BciVI BcnI BfaI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII