##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
BbeI BcgI BciVI BfrI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII BstAPI BstBI BstEII BstPI
ClaI CpoI DrdI Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HindIII KasI KspI MfeI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 266 GACGTC GACGT^C
Acc65I 3015 GGTACC G^GTACC
AcyI 266 GACGTC G[GA]^CG[CT]C
AflIII 1280 ACGCGT A^C[GA][CT]GT
Asp718I 3015 GGTACC G^GTACC
AvaI 3394 CTCGGG C^[CT]CG[GA]G
AvrII 3268 CCTAGG C^CTAGG
BamHI 1751 GGATCC G^GATCC
BbrPI 2536 CACGTG CAC^GTG
BbvCI 1545 GCTGAGG CCTCAGC(-5/-2)
BclI 1442 TGATCA T^GATCA
BlnI 3268 CCTAGG C^CTAGG
BsaAI 2536 CACGTG [CT]AC^GT[GA]
BsaBI 1142 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 266 GACGTC G[GA]^CG[CT]C
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsmBI 264 GAGACG CGTCTC(1/5)
BsoBI 3394 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2658 TGTACA T^GTACA
BspHI 4225 TCATGA T^CATGA
BsrFI 73 GCCGGC [GA]^CCGG[CT]
BsrGI 2658 TGTACA T^GTACA
BssSI 3790 CACGAG CACGAG(-5/-1)
Cfr10I 73 GCCGGC [GA]^CCGG[CT]
DraIII 2323 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 71 CGGCCG C^GGCCG
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
FbaI 1442 TGATCA T^GATCA
Hin1I 266 GACGTC G[GA]^CG[CT]C
HincII 4172 GTTAAC GT[CT]^[GA]AC
HindII 4172 GTTAAC GT[CT]^[GA]AC
HpaI 4172 GTTAAC GTT^AAC
KpnI 3015 GGTACC GGTAC^C
MamI 1142 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1280 ACGCGT A^CGCGT
NaeI 73 GCCGGC GCC^GGC
NgoMIV 73 GCCGGC G^CCGGC
PflMI 1742 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2536 CACGTG CAC^GTG
PmlI 2536 CACGTG CAC^GTG
RcaI 4225 TCATGA T^CATGA
SapI 3042 GCTCTTC GCTCTTC(1/4)
SbfI 2969 CCTGCAGG CCTGCA^GG
ScaI 2755 AGTACT AGT^ACT
Sse8387I 2969 CCTGCAGG CCTGCA^GG
SspBI 2658 TGTACA T^GTACA
Van91I 1742 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1382 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 556 GGGCCC GGGCC^C
2317 GGGCCC
BalI 2410 TGGCCA TGG^CCA
3009 TGGCCA
BsaI 1801 GAGACC GGTCTC(1/5)
2492 GGTCTC
BsmI 1838 GAATGC GAATGC(1/-1)
2424 GCATTC
BsrBI 965 CCGCTC CCGCTC(-3/-3)
2554 GAGCGG
Bst1107I 3105 GTATAC GTA^TAC
3554 GTATAC
BstZ17I 3105 GTATAC GTA^TAC
3554 GTATAC
CfoI 203 GCGC GCG^C
2399 GCGC
EcoRI 1314 GAATTC G^AATTC
1916 GAATTC
HgaI 121 GCGTC GACGC(5/10)
2155 GCGTC
HhaI 203 GCGC GCG^C
2399 GCGC
HinP1I 203 GCGC G^CGC
2399 GCGC
MluNI 2410 TGGCCA TGG^CCA
3009 TGGCCA
MscI 2410 TGGCCA TGG^CCA
3009 TGGCCA
PpuMI 1549 AGGACCT [GA]G^G[AT]CC[CT]
4212 GGGACCT
PstI 244 CTGCAG CTGCA^G
2970 CTGCAG
SmlI 814 CTCAAG C^T[CT][GA]AG
2815 CTTGAG
SphI 1366 GCATGC GCATG^C
2140 GCATGC
StuI 2873 AGGCCT AGG^CCT
3265 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII