##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        Alw44I      ApaLI       AscI        
Asp718I     AspI        AviII       BbeI        BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BpuAI       BsaHI       BsgI        
BsiWI       BsmBI       BspDI       BspHI       BsrFI       
BssHII      BstAPI      BstEII      BstPI       BstUI       
Cfr10I      ClaI        DraI        EcoO65I     EcoT22I     
FbaI        FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MunI        MvnI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmeI        PpuMI       PshAI       Psp1406I    PvuI        
RcaI        SacII       SalI        SapI        SbfI        
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   71 enzymes) #
###################################

AccIII         80  TCCGGA               T^CCGGA
AhdI           29  GACTTGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI           45  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2445  AGCGCT               AGC^GCT
ApaI          104  GGGCCC               GGGCC^C
AspEI          29  GACTTGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          373  CTCGGG               C^[CT]CG[GA]G
AvrII        2791  CCTAGG               C^CTAGG
BalI         1377  TGGCCA               TGG^CCA
BamHI         368  GGATCC               G^GATCC
BanI          965  GGCACC               G^G[CT][GA]CC
BbrPI        2217  CACGTG               CAC^GTG
BglII        2745  AGATCT               A^GATCT
BlnI         2791  CCTAGG               C^CTAGG
BlpI         2211  GCTCAGC              GC^T[ACGT]AGC
BpmI          680  CTGGAG               CTGGAG(16/14)
Bpu1102I     2211  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2217  CACGTG               [CT]AC^GT[GA]
BsaBI        2134  GATTGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1119  GGTCTC               GGTCTC(1/5)
BsaWI          80  TCCGGA               [AT]^CCGG[AT]
BseAI          80  TCCGGA               T^CCGGA
BsiEI         132  CGGCCG               CG[GA][CT]^CG
BsmI          672  GAATGC               GAATGC(1/-1)
BsoBI         373  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1087  TGTACA               T^GTACA
BspEI          80  TCCGGA               T^CCGGA
BspLU11I     1186  ACATGT               A^CATGT
BspMI        2856  GCAGGT               ACCTGC(4/8)
BsrBI         387  GAGCGG               CCGCTC(-3/-3)
BsrGI        1087  TGTACA               T^GTACA
BssSI          88  CTCGTG               CACGAG(-5/-1)
Bst1107I     1384  GTATAC               GTA^TAC
BstBI        1569  TTCGAA               TT^CGAA
BstZ17I      1384  GTATAC               GTA^TAC
Bsu36I         45  CCTGAGG              CC^T[ACGT]AGG
CelII        2211  GCTCAGC              GC^T[ACGT]AGC
CpoI           77  CGGTCCG              CG^G[AT]CCG
DrdI          284  GACAGGCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          132  CGGCCG               C^GGCCG
Eam1105I       29  GACTTGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         132  CGGCCG               C^GGCCG
Eco47III     2445  AGCGCT               AGC^GCT
Eco52I        132  CGGCCG               C^GGCCG
Eco81I         45  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2637  CCTGTTCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1287  GAATTC               G^AATTC
EcoRV        2138  GATATC               GAT^ATC
HapII          81  CCGG                 C^CGG
HgaI         2939  GCGTC                GACGC(5/10)
HincII       2604  GTCAAC               GT[CT]^[GA]AC
HindII       2604  GTCAAC               GT[CT]^[GA]AC
HpaII          81  CCGG                 C^CGG
MamI         2134  GATTGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1377  TGGCCA               TGG^CCA
MroI           80  TCCGGA               T^CCGGA
MscI         1377  TGGCCA               TGG^CCA
MspI           81  CCGG                 C^CGG
NcoI         1808  CCATGG               C^CATGG
NspV         1569  TTCGAA               TT^CGAA
PmaCI        2217  CACGTG               CAC^GTG
PmlI         2217  CACGTG               CAC^GTG
RsrII          77  CGGTCCG              CG^G[AT]CCG
SacI         1949  GAGCTC               GAGCT^C
ScaI          842  AGTACT               AGT^ACT
SexAI        2864  ACCTGGT              A^CC[AT]GGT
SfuI         1569  TTCGAA               TT^CGAA
SspBI        1087  TGTACA               T^GTACA
SspI          580  AATATT               AAT^ATT
XbaI         1847  TCTAGA               T^CTAGA
XcmI         1502  CCAGATGAAAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         1384  GTATAC               GT^[AC][GT]AC
             2726  GTAGAC              
AflIII       1186  ACATGT               A^C[GA][CT]GT
             2873  ACACGT              
AlwNI          40  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              161  CAGAAGCTG           
AseI          748  ATTAAT               AT^TAAT
             1895  ATTAAT              
AsnI          748  ATTAAT               AT^TAAT
             1895  ATTAAT              
BseRI        2806  CTCCTC               GAGGAG(10/8)
             2375  GAGGAG              
BsrDI        2055  CATTGC               GCAATG(2/0)
             1860  GCAATG              
BstXI        1219  CCAGAGGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1575  CCAACTGTGTGG        
DraIII        756  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2863  CACCTGGTG           
DsaI          129  CCACGG               C^C[GA][CT]GG
             1808  CCATGG              
FauI         2359  CCCGC                CCCGC(4/6)
              768  GCGGG               
HaeII        2354  AGCGCC               [GA]GCGC^[CT]
             2445  AGCGCT              
MaeII        2218  ACGT                 A^CGT
             2875  ACGT                
NdeI         1083  CATATG               CA^TATG
             2163  CATATG              
NspI          289  GCATGT               [GA]CATG^[CT]
             1186  ACATGT              
PflMI         160  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2862  CCACCTGGTGG         
PshBI         748  ATTAAT               AT^TAAT
             1895  ATTAAT              
PstI         1065  CTGCAG               CTGCA^G
             1553  CTGCAG              
PvuII        1646  CAGCTG               CAG^CTG
             2591  CAGCTG              
Van91I        160  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2862  CCACCTGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaII       BanII       
BbvI        BfaI        BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI