##################################
# NO CUT ENZYMES ( 31 enzymes) #
##################################
AclI AscI AviII BsiEI BsiWI
BssHII BssSI BstBI CpoI EagI
EclXI Eco52I FspI MfeI MluI
MunI NotI NspV PacI PmeI
Psp1406I PvuI RsrII SapI SbfI
SfiI SfuI SnaBI SpeI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 9720 GGTACC G^GTACC
AflII 6792 CTTAAG C^TTAAG
AgeI 8307 ACCGGT A^CCGGT
Aor51HI 4141 AGCGCT AGC^GCT
Asp718I 9720 GGTACC G^GTACC
BbeI 156 GGCGCC GGCGC^C
BbrPI 1088 CACGTG CAC^GTG
BbvCI 116 CCTCAGC CCTCAGC(-5/-2)
BcgI 7115 CGATGAACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2940 GGATAC GTATCC(6/5)
BfrI 6792 CTTAAG C^TTAAG
BspDI 2839 ATCGAT AT^CGAT
ClaI 2839 ATCGAT AT^CGAT
Eco47III 4141 AGCGCT AGC^GCT
EcoRV 10372 GATATC GAT^ATC
EcoT22I 9454 ATGCAT ATGCA^T
FseI 5068 GGCCGGCC GGCCGG^CC
HpaI 11972 GTTAAC GTT^AAC
KasI 156 GGCGCC G^GCGCC
KpnI 9720 GGTACC GGTAC^C
NarI 156 GGCGCC GG^CGCC
NdeI 6518 CATATG CA^TATG
NheI 11952 GCTAGC G^CTAGC
NruI 6649 TCGCGA TCG^CGA
NsiI 9454 ATGCAT ATGCA^T
PaeR7I 1697 CTCGAG C^TCGAG
PinAI 8307 ACCGGT A^CCGGT
PmaCI 1088 CACGTG CAC^GTG
PmlI 1088 CACGTG CAC^GTG
SalI 1110 GTCGAC G^TCGAC
ScaI 6022 AGTACT AGT^ACT
SfoI 156 GGCGCC GGC^GCC
SgrAI 6 CGCCGGCG C[GA]^CCGG[CT]G
SphI 5464 GCATGC GCATG^C
XhoI 1697 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 2568 GACGTC GACGT^C
6451 GACGTC
AccIII 1726 TCCGGA T^CCGGA
7741 TCCGGA
AhdI 2969 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8611 GACCCTAGGTC
AspEI 2969 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8611 GACCCTAGGTC
AspI 630 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6660 GACTTTGTC
BseAI 1726 TCCGGA T^CCGGA
7741 TCCGGA
BsmBI 593 CGTCTC CGTCTC(1/5)
4001 CGTCTC
BspEI 1726 TCCGGA T^CCGGA
7741 TCCGGA
BspLU11I 8163 ACATGT A^CATGT
10189 ACATGT
Bst1107I 4380 GTATAC GTA^TAC
10185 GTATAC
BstZ17I 4380 GTATAC GTA^TAC
10185 GTATAC
DrdI 3102 GACACGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3120 GACATTAAAGTC
Eam1105I 2969 GACTCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8611 GACCCTAGGTC
EcoRI 2282 GAATTC G^AATTC
6305 GAATTC
HgaI 9875 GACGC GACGC(5/10)
874 GCGTC
HindIII 762 AAGCTT A^AGCTT
8442 AAGCTT
KspI 57 CCGCGG CCGC^GG
2594 CCGCGG
MroI 1726 TCCGGA T^CCGGA
7741 TCCGGA
NaeI 7 GCCGGC GCC^GGC
5069 GCCGGC
NgoMIV 7 GCCGGC G^CCGGC
5069 GCCGGC
PflFI 630 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6660 GACTTTGTC
PflMI 686 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4065 CCACAGGATGG
PshAI 11266 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
12163 GACCAAAGTC
SacII 57 CCGCGG CCGC^GG
2594 CCGCGG
Tth111I 630 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6660 GACTTTGTC
Van91I 686 CCAAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4065 CCACAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 166 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII AvrII BalI BamHI BanI
BanII BbsI BbvI BclI BcnI
BfaI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PshBI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII XmaCI XmaI
XmnI