##################################
# NO CUT ENZYMES ( 31 enzymes) #
##################################
AatII AclI AgeI AocI AscI
AviII BciVI BsiWI BspDI Bsu36I
ClaI CpoI Eco81I EcoRV FseI
FspI HpaI MfeI MluI MunI
NotI NruI PacI PinAI PshAI
Psp1406I RsrII SnaBI SphI SwaI
XbaI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 3653 GGTACC G^GTACC
AccIII 1684 TCCGGA T^CCGGA
Aor51HI 404 AGCGCT AGC^GCT
AseI 6046 ATTAAT AT^TAAT
AsnI 6046 ATTAAT AT^TAAT
Asp718I 3653 GGTACC G^GTACC
AvrII 5885 CCTAGG C^CTAGG
BcgI 5112 CGATCGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 7554 TGATCA T^GATCA
BglII 4423 AGATCT A^GATCT
BlnI 5885 CCTAGG C^CTAGG
BseAI 1684 TCCGGA T^CCGGA
BspEI 1684 TCCGGA T^CCGGA
BspLU11I 3368 ACATGT A^CATGT
Bst1107I 7750 GTATAC GTA^TAC
BstBI 759 TTCGAA TT^CGAA
BstZ17I 7750 GTATAC GTA^TAC
DraIII 6429 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 404 AGCGCT AGC^GCT
EcoNI 5726 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 7554 TGATCA T^GATCA
HindIII 3259 AAGCTT A^AGCTT
KpnI 3653 GGTACC GGTAC^C
MroI 1684 TCCGGA T^CCGGA
NspV 759 TTCGAA TT^CGAA
PflMI 834 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 6061 GTTTAAAC GTTT^AAAC
PshBI 6046 ATTAAT AT^TAAT
SalI 1455 GTCGAC G^TCGAC
SexAI 2228 ACCTGGT A^CC[AT]GGT
SfiI 6737 GGCCTCCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 759 TTCGAA TT^CGAA
SpeI 5932 ACTAGT A^CTAGT
SspI 5254 AATATT AAT^ATT
Van91I 834 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 4785 CTTAAG C^TTAAG
7915 CTTAAG
AspI 2184 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7105 GACCACGTC
BbsI 7028 GAAGAC GAAGAC(2/6)
3136 GTCTTC
BbvCI 196 GCTGAGG CCTCAGC(-5/-2)
2604 GCTGAGG
BfrI 4785 CTTAAG C^TTAAG
7915 CTTAAG
BlpI 4096 GCTCAGC GC^T[ACGT]AGC
8388 GCTGAGC
Bpu1102I 4096 GCTCAGC GC^T[ACGT]AGC
8388 GCTGAGC
BpuAI 7028 GAAGAC GAAGAC(2/6)
3136 GTCTTC
BsaBI 1053 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3291 GATCCTCATC
BsaWI 1355 ACCGGA [AT]^CCGG[AT]
1684 TCCGGA
Bsp1407I 3365 TGTACA T^GTACA
5710 TGTACA
BspHI 627 TCATGA T^CATGA
7818 TCATGA
BsrGI 3365 TGTACA T^GTACA
5710 TGTACA
BstEII 616 GGTTACC G^GT[ACGT]ACC
3957 GGTCACC
BstPI 616 GGTTACC G^GT[ACGT]ACC
3957 GGTCACC
CelII 4096 GCTCAGC GC^T[ACGT]AGC
8388 GCTGAGC
EcoO65I 616 GGTTACC G^GT[ACGT]ACC
3957 GGTCACC
MamI 1053 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3291 GATCCTCATC
NdeI 3072 CATATG CA^TATG
5528 CATATG
NheI 786 GCTAGC G^CTAGC
6721 GCTAGC
PflFI 2184 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7105 GACCACGTC
PvuI 3542 CGATCG CGAT^CG
5112 CGATCG
RcaI 627 TCATGA T^CATGA
7818 TCATGA
ScaI 3509 AGTACT AGT^ACT
3869 AGTACT
SmaI 2675 CCCGGG CCC^GGG
4606 CCCGGG
SspBI 3365 TGTACA T^GTACA
5710 TGTACA
Tth111I 2184 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7105 GACCACGTC
XmaCI 2675 CCCGGG C^CCGGG
4606 CCCGGG
XmaI 2675 CCCGGG C^CCGGG
4606 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 163 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI Alw44I
AlwI AlwNI ApaI ApaLI ApoI
Asp700I AspEI AspHI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbrPI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NsiI NspI PaeR7I PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfoI SgrAI SmlI Sse8387I StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoI XhoII XmnI