################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AccIII AcyI AgeI Alw44I AocI Aor51HI ApaLI AscI AviII AvrII BamHI BbeI BbrPI BbvCI BglI BlnI BsaAI BsaHI BseAI BsiWI BsmBI BspDI BspEI BspHI BssSI BstBI Bsu36I ClaI CpoI Eco47III Eco81I FseI FspI HaeII Hin1I KasI KspI MluI MroI NarI NheI NotI NruI NspV PinAI PmaCI PmeI PmlI PshAI PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 5111 GGTACC G^GTACC ApaI 1837 GGGCCC GGGCC^C Asp700I 3770 GAATAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 5111 GGTACC G^GTACC BcgI 1233 GCAGGCATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1522 AGATCT A^GATCT BlpI 5781 GCTTAGC GC^T[ACGT]AGC Bpu1102I 5781 GCTTAGC GC^T[ACGT]AGC BsaBI 635 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 16 ACCGGA [AT]^CCGG[AT] BsiEI 1596 CGGCCG CG[GA][CT]^CG BsrBI 1493 GAGCGG CCGCTC(-3/-3) BssHII 1246 GCGCGC G^CGCGC Bst1107I 4181 GTATAC GTA^TAC BstEII 6512 GGTGACC G^GT[ACGT]ACC BstPI 6512 GGTGACC G^GT[ACGT]ACC BstZ17I 4181 GTATAC GTA^TAC CelII 5781 GCTTAGC GC^T[ACGT]AGC DraIII 2660 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1596 CGGCCG C^GGCCG EclXI 1596 CGGCCG C^GGCCG Eco52I 1596 CGGCCG C^GGCCG EcoNI 5955 CCTTCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 6512 GGTGACC G^GT[ACGT]ACC EcoT22I 4151 ATGCAT ATGCA^T FauI 1481 GCGGG CCCGC(4/6) KpnI 5111 GGTACC GGTAC^C MamI 635 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 5006 GCCGGC GCC^GGC NcoI 6553 CCATGG C^CATGG NdeI 3365 CATATG CA^TATG NgoMIV 5006 GCCGGC G^CCGGC NsiI 4151 ATGCAT ATGCA^T PaeR7I 1047 CTCGAG C^TCGAG SapI 1073 GAAGAGC GCTCTTC(1/4) SmaI 1540 CCCGGG CCC^GGG XbaI 7045 TCTAGA T^CTAGA XhoI 1047 CTCGAG C^TCGAG XmaCI 1540 CCCGGG C^CCGGG XmaI 1540 CCCGGG C^CCGGG XmnI 3770 GAATAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AclI 2217 AACGTT AA^CGTT 3566 AACGTT AflII 3410 CTTAAG C^TTAAG 4616 CTTAAG AhdI 508 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2393 GACACTTGGTC AspEI 508 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2393 GACACTTGGTC AspI 1470 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5652 GACATAGTC BciVI 640 GTATCC GTATCC(6/5) 2381 GTATCC BclI 2606 TGATCA T^GATCA 6039 TGATCA BfrI 3410 CTTAAG C^TTAAG 4616 CTTAAG Bsp1407I 1585 TGTACA T^GTACA 2231 TGTACA BspLU11I 1582 ACATGT A^CATGT 2895 ACATGT BsrDI 182 CATTGC GCAATG(2/0) 259 GCAATG BsrFI 1594 ACCGGC [GA]^CCGG[CT] 5006 GCCGGC BsrGI 1585 TGTACA T^GTACA 2231 TGTACA Cfr10I 1594 ACCGGC [GA]^CCGG[CT] 5006 GCCGGC DrdI 1923 GACCTCCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5469 GACAGAAGGGTC DsaI 2428 CCACGG C^C[GA][CT]GG 6553 CCATGG Eam1105I 508 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2393 GACACTTGGTC EcoRI 4835 GAATTC G^AATTC 5309 GAATTC EcoRV 3556 GATATC GAT^ATC 5770 GATATC FbaI 2606 TGATCA T^GATCA 6039 TGATCA HindIII 5041 AAGCTT A^AGCTT 5602 AAGCTT HpaI 756 GTTAAC GTT^AAC 5289 GTTAAC MfeI 511 CAATTG C^AATTG 5090 CAATTG MunI 511 CAATTG C^AATTG 5090 CAATTG PacI 3323 TTAATTAA TTAAT^TAA 3745 TTAATTAA PflFI 1470 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5652 GACATAGTC PflMI 584 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2919 CCAATACTTGG Psp1406I 2217 AACGTT AA^CGTT 3566 AACGTT SexAI 1914 ACCAGGT A^CC[AT]GGT 2952 ACCAGGT SpeI 3353 ACTAGT A^CTAGT 3464 ACTAGT SphI 1397 GCATGC GCATG^C 5130 GCATGC SspBI 1585 TGTACA T^GTACA 2231 TGTACA StuI 171 AGGCCT AGG^CCT 1124 AGGCCT Tth111I 1470 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 5652 GACATAGTC Van91I 584 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2919 CCAATACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII