##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI ApaI AscI BbeI BbrPI
BciVI BfrI BglI BglII BseAI
BsiWI BsmBI BspEI BsrBI Bst1107I
BstAPI BstZ17I CpoI DraIII DrdI
Eco47III EcoRV FseI HpaI KasI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI Psp1406I RsrII SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 5227 GGCGTC G[GA]^CG[CT]C
AocI 2990 CCTCAGG CC^T[ACGT]AGG
AviII 3061 TGCGCA TGC^GCA
AvrII 1610 CCTAGG C^CTAGG
BamHI 2328 GGATCC G^GATCC
BclI 670 TGATCA T^GATCA
BlnI 1610 CCTAGG C^CTAGG
BlpI 791 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 791 GCTAAGC GC^T[ACGT]AGC
BsaAI 1875 CACGTA [CT]AC^GT[GA]
BsaHI 5227 GGCGTC G[GA]^CG[CT]C
BspHI 1225 TCATGA T^CATGA
BssHII 76 GCGCGC G^CGCGC
BstEII 4069 GGTGACC G^GT[ACGT]ACC
BstPI 4069 GGTGACC G^GT[ACGT]ACC
BstXI 4417 CCATGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2990 CCTCAGG CC^T[ACGT]AGG
CelII 791 GCTAAGC GC^T[ACGT]AGC
Eco81I 2990 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4069 GGTGACC G^GT[ACGT]ACC
EcoRI 2696 GAATTC G^AATTC
FbaI 670 TGATCA T^GATCA
FspI 3061 TGCGCA TGC^GCA
HaeII 40 GGCGCT [GA]GCGC^[CT]
Hin1I 5227 GGCGTC G[GA]^CG[CT]C
MfeI 3507 CAATTG C^AATTG
MunI 3507 CAATTG C^AATTG
PshAI 5007 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1897 CGATCG CGAT^CG
RcaI 1225 TCATGA T^CATGA
StuI 4682 AGGCCT AGG^CCT
XbaI 406 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 3234 ACATGT A^C[GA][CT]GT
4366 ACATGT
Alw44I 198 GTGCAC G^TGCAC
5265 GTGCAC
AlwNI 801 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
3167 CAGCAGCTG
ApaLI 198 GTGCAC G^TGCAC
5265 GTGCAC
AseI 1088 ATTAAT AT^TAAT
4480 ATTAAT
AsnI 1088 ATTAAT AT^TAAT
4480 ATTAAT
AspI 322 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1761 GACATTGTC
AvaI 317 CCCGAG C^[CT]CG[GA]G
335 CCCGAG
BbsI 410 GAAGAC GAAGAC(2/6)
4992 GAAGAC
BcgI 2920 GCACGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4134 GCATCAGCGTCG
BpuAI 410 GAAGAC GAAGAC(2/6)
4992 GAAGAC
BsaBI 746 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2897 GATGCCTATC
BsoBI 317 CCCGAG C^[CT]CG[GA]G
335 CCCGAG
BspDI 2894 ATCGAT AT^CGAT
3753 ATCGAT
BspLU11I 3234 ACATGT A^CATGT
4366 ACATGT
BsrFI 48 GCCGGT [GA]^CCGG[CT]
3145 GCCGGT
BssSI 5077 CACGAG CACGAG(-5/-1)
4599 CTCGTG
BstBI 2039 TTCGAA TT^CGAA
4235 TTCGAA
Cfr10I 48 GCCGGT [GA]^CCGG[CT]
3145 GCCGGT
ClaI 2894 ATCGAT AT^CGAT
3753 ATCGAT
DraI 871 TTTAAA TTT^AAA
2368 TTTAAA
EagI 46 CGGCCG C^GGCCG
4629 CGGCCG
EclXI 46 CGGCCG C^GGCCG
4629 CGGCCG
Eco52I 46 CGGCCG C^GGCCG
4629 CGGCCG
EcoNI 1863 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3611 CCTGTTACAGG
EcoT22I 3183 ATGCAT ATGCA^T
3577 ATGCAT
KspI 71 CCGCGG CCGC^GG
178 CCGCGG
MamI 746 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2897 GATGCCTATC
NsiI 3183 ATGCAT ATGCA^T
3577 ATGCAT
NspV 2039 TTCGAA TT^CGAA
4235 TTCGAA
PflFI 322 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1761 GACATTGTC
PshBI 1088 ATTAAT AT^TAAT
4480 ATTAAT
SacI 2612 GAGCTC GAGCT^C
4280 GAGCTC
SacII 71 CCGCGG CCGC^GG
178 CCGCGG
SalI 1112 GTCGAC G^TCGAC
4142 GTCGAC
SapI 1322 GCTCTTC GCTCTTC(1/4)
2614 GCTCTTC
SfuI 2039 TTCGAA TT^CGAA
4235 TTCGAA
SpeI 2540 ACTAGT A^CTAGT
3043 ACTAGT
SphI 1018 GCATGC GCATG^C
3021 GCATGC
SspI 4231 AATATT AAT^ATT
4933 AATATT
Tth111I 322 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1761 GACATTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AhdI AluI AlwI ApoI
Asp700I Asp718I AspEI AspHI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI