##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AhdI        
AocI        ApaI        AscI        AspEI       AspI        
BbeI        BbrPI       BclI        BglII       BlpI        
Bpu1102I    BsaHI       BseAI       BsiWI       BsmBI       
BspDI       BspEI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       Bsu36I      CelII       
ClaI        CpoI        Eam1105I    Eco81I      EcoO65I     
FbaI        FseI        Hin1I       KasI        KspI        
MluI        MroI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NspV        PaeR7I      
PflFI       PflMI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        PvuII       RsrII       
SacII       SalI        SapI        SbfI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    StuI        Tth111I     Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I       5978  GGTACC               G^GTACC
AccI         4065  GTATAC               GT^[AC][GT]AC
AccII         173  CGCG                 CG^CG
AgeI         1919  ACCGGT               A^CCGGT
Aor51HI         7  AGCGCT               AGC^GCT
Asp718I      5978  GGTACC               G^GTACC
AviII        4673  TGCGCA               TGC^GCA
BanII        4848  GAGCTC               G[GA]GC[CT]^C
BcgI         5583  CGATTCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2588  GGATAC               GTATCC(6/5)
BsaBI        2184  GATCCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1919  ACCGGT               [AT]^CCGG[AT]
Bsp1407I     1563  TGTACA               T^GTACA
BspMI        5975  GCAGGT               ACCTGC(4/8)
BsrFI        1919  ACCGGT               [GA]^CCGG[CT]
BsrGI        1563  TGTACA               T^GTACA
Bst1107I     4065  GTATAC               GTA^TAC
BstUI         173  CGCG                 CG^CG
BstZ17I      4065  GTATAC               GTA^TAC
Cfr10I       1919  ACCGGT               [GA]^CCGG[CT]
DrdI          440  GACGCTTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III        7  AGCGCT               AGC^GCT
EcoNI         243  CCTTCGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2090  GAATTC               G^AATTC
EcoRV        5915  GATATC               GAT^ATC
FspI         4673  TGCGCA               TGC^GCA
HaeII           7  AGCGCT               [GA]GCGC^[CT]
KpnI         5978  GGTACC               GGTAC^C
MamI         2184  GATCCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI          173  CGCG                 CG^CG
NcoI         6037  CCATGG               C^CATGG
PacI         1090  TTAATTAA             TTAAT^TAA
PinAI        1919  ACCGGT               A^CCGGT
SacI         4848  GAGCTC               GAGCT^C
SexAI        6105  ACCAGGT              A^CC[AT]GGT
SfiI           58  GGCCTGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI        1563  TGTACA               T^GTACA
SwaI          843  ATTTAAAT             ATTT^AAAT
XbaI         5919  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AflII        1032  CTTAAG               C^TTAAG
             4461  CTTAAG              
BamHI         311  GGATCC               G^GATCC
             1129  GGATCC              
BbvCI        5594  CCTCAGC              CCTCAGC(-5/-2)
             2042  GCTGAGG             
BfrI         1032  CTTAAG               C^TTAAG
             4461  CTTAAG              
BglI           59  GCCTGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              170  GCCCGCGTGGC         
BsaAI        1270  CACGTA               [CT]AC^GT[GA]
             6215  TACGTG              
BsiEI         149  CGGCCG               CG[GA][CT]^CG
              293  CGGCCG              
BsrBI         366  CCGCTC               CCGCTC(-3/-3)
              384  CCGCTC              
BstXI        2578  CCAGATAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3166  CCATTTTTATGG        
DraIII       4289  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4330  CACAGGGTG           
DsaI          296  CCGTGG               C^C[GA][CT]GG
             6037  CCATGG              
EagI          149  CGGCCG               C^GGCCG
              293  CGGCCG              
EarI         1302  CTCTTC               CTCTTC(1/4)
             4792  CTCTTC              
EclXI         149  CGGCCG               C^GGCCG
              293  CGGCCG              
Eco52I        149  CGGCCG               C^GGCCG
              293  CGGCCG              
EcoT22I      3566  ATGCAT               ATGCA^T
             4446  ATGCAT              
FauI          171  CCCGC                CCCGC(4/6)
              371  CCCGC               
Ksp632I      1302  CTCTTC               CTCTTC(1/4)
             4792  CTCTTC              
NdeI         4162  CATATG               CA^TATG
             4688  CATATG              
NsiI         3566  ATGCAT               ATGCA^T
             4446  ATGCAT              
PpuMI        2050  AGGACCT              [GA]G^G[AT]CC[CT]
             5459  AGGACCT             
SmaI           22  CCCGGG               CCC^GGG
             5571  CCCGGG              
SmlI         1032  CTTAAG               C^T[CT][GA]AG
             4461  CTTAAG              
XcmI         1317  CCAGTATAGCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1985  CCACTGAAGAAGTGG     
XmaCI          22  CCCGGG               C^CCGGG
             5571  CCCGGG              
XmaI           22  CCCGGG               C^CCGGG
             5571  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
AseI        AsnI        Asp700I     AspHI       AvaI        
AvaII       AvrII       BalI        BanI        BbsI        
BbvI        BcnI        BfaI        BlnI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspHI       
BspLU11I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       EaeI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MfeI        MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MunI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PshBI       PstI        RcaI        RsaI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI