##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI ApaI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BfrI BglI BlnI BsaAI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I FseI FspI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I StuI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 4395 GACGTC GACGT^C
AccI 1566 GTAGAC GT^[AC][GT]AC
AcyI 4395 GACGTC G[GA]^CG[CT]C
Alw44I 5120 GTGCAC G^TGCAC
Aor51HI 4918 AGCGCT AGC^GCT
ApaLI 5120 GTGCAC G^TGCAC
Asp700I 5505 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 2909 GGCACC G^G[CT][GA]CC
BanII 2818 GAGCTC G[GA]GC[CT]^C
BbvCI 3329 GCTGAGG CCTCAGC(-5/-2)
BcnI 4618 CCCGG CC^[GC]GG
BglII 3266 AGATCT A^GATCT
BsaHI 4395 GACGTC G[GA]^CG[CT]C
BsgI 5879 GTGCAG GTGCAG(16/14)
BsmI 5968 GCATTC GAATGC(1/-1)
BstBI 3645 TTCGAA TT^CGAA
DraIII 3858 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2947 CCATGG C^C[GA][CT]GG
Eco47III 4918 AGCGCT AGC^GCT
EcoNI 1759 CCTTGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 766 GAATTC G^AATTC
FauI 2096 GCGGG CCCGC(4/6)
HaeII 4918 AGCGCT [GA]GCGC^[CT]
HapII 4619 CCGG C^CGG
HgaI 257 GCGTC GACGC(5/10)
Hin1I 4395 GACGTC G[GA]^CG[CT]C
HindIII 850 AAGCTT A^AGCTT
HpaI 5884 GTTAAC GTT^AAC
HpaII 4619 CCGG C^CGG
MspI 4619 CCGG C^CGG
NciI 4618 CCCGG CC^[GC]GG
NcoI 2947 CCATGG C^CATGG
NspV 3645 TTCGAA TT^CGAA
PmeI 3529 GTTTAAAC GTTT^AAAC
SacI 2818 GAGCTC GAGCT^C
SapI 3049 GAAGAGC GCTCTTC(1/4)
SfuI 3645 TTCGAA TT^CGAA
SwaI 5974 ATTTAAAT ATTT^AAAT
XbaI 5501 TCTAGA T^CTAGA
XmnI 5505 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AseI 946 ATTAAT AT^TAAT
1544 ATTAAT
AsnI 946 ATTAAT AT^TAAT
1544 ATTAAT
AspHI 2818 GAGCTC G[AT]GC[AT]^C
5120 GTGCAC
BsaI 1839 GAGACC GGTCTC(1/5)
1313 GGTCTC
BseRI 101 CTCCTC GAGGAG(10/8)
4791 CTCCTC
BsiHKAI 2818 GAGCTC G[AT]GC[AT]^C
5120 GTGCAC
BspHI 3904 TCATGA T^CATGA
5035 TCATGA
BspMI 4405 ACCTGC ACCTGC(4/8)
5005 ACCTGC
BstAPI 3010 GCAACAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4258 GCATCCCCTGC
BstEII 3602 GGTAACC G^GT[ACGT]ACC
6276 GGTCACC
BstPI 3602 GGTAACC G^GT[ACGT]ACC
6276 GGTCACC
CfoI 1137 GCGC GCG^C
4919 GCGC
EcoO65I 3602 GGTAACC G^GT[ACGT]ACC
6276 GGTCACC
EcoRV 3379 GATATC GAT^ATC
3503 GATATC
EcoT14I 2947 CCATGG C^C[AT][AT]GG
4113 CCAAGG
HhaI 1137 GCGC GCG^C
4919 GCGC
HinP1I 1137 GCGC G^CGC
4919 GCGC
PpuMI 4477 AGGTCCT [GA]G^G[AT]CC[CT]
6305 AGGACCT
PshBI 946 ATTAAT AT^TAAT
1544 ATTAAT
PstI 591 CTGCAG CTGCA^G
2645 CTGCAG
RcaI 3904 TCATGA T^CATGA
5035 TCATGA
SpeI 2234 ACTAGT A^CTAGT
3071 ACTAGT
StyI 2947 CCATGG C^C[AT][AT]GG
4113 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AvaII
BalI BbsI BbvI BclI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoO109I EcoRII EcoT22I
FbaI Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MunI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII