##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
Alw44I ApaLI AscI Asp700I AspEI
AvrII BamHI BbeI BbvCI BglI
BlnI BsaI BseAI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
BstBI BstUI ClaI CpoI DrdI
Eam1105I FseI HindIII KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 424 GACGTC GACGT^C
AcyI 424 GACGTC G[GA]^CG[CT]C
AflII 3985 CTTAAG C^TTAAG
Aor51HI 2665 AGCGCT AGC^GCT
ApaI 3066 GGGCCC GGGCC^C
AspI 1050 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2612 TGCGCA TGC^GCA
BbrPI 1385 CACGTG CAC^GTG
BcgI 2694 GCAGGAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2382 GGATAC GTATCC(6/5)
BfrI 3985 CTTAAG C^TTAAG
BsaBI 690 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 424 GACGTC G[GA]^CG[CT]C
BsaWI 5470 TCCGGT [AT]^CCGG[AT]
BsiEI 78 CGGCCG CG[GA][CT]^CG
BspHI 4001 TCATGA T^CATGA
BsrFI 553 ACCGGC [GA]^CCGG[CT]
Bst1107I 3713 GTATAC GTA^TAC
BstEII 1298 GGTTACC G^GT[ACGT]ACC
BstPI 1298 GGTTACC G^GT[ACGT]ACC
BstXI 5516 CCATCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3713 GTATAC GTA^TAC
Cfr10I 553 ACCGGC [GA]^CCGG[CT]
DsaI 4658 CCATGG C^C[GA][CT]GG
EagI 78 CGGCCG C^GGCCG
EclXI 78 CGGCCG C^GGCCG
Eco47III 2665 AGCGCT AGC^GCT
Eco52I 78 CGGCCG C^GGCCG
EcoO65I 1298 GGTTACC G^GT[ACGT]ACC
EcoRV 901 GATATC GAT^ATC
EcoT22I 746 ATGCAT ATGCA^T
FspI 2612 TGCGCA TGC^GCA
Hin1I 424 GACGTC G[GA]^CG[CT]C
MamI 690 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 4658 CCATGG C^CATGG
NdeI 3854 CATATG CA^TATG
NsiI 746 ATGCAT ATGCA^T
PflFI 1050 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1385 CACGTG CAC^GTG
PmlI 1385 CACGTG CAC^GTG
RcaI 4001 TCATGA T^CATGA
SpeI 3571 ACTAGT A^CTAGT
Tth111I 1050 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1562 TCTAGA T^CTAGA
XcmI 2194 CCACTCGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1080 GGTACC G^GTACC
2272 GGTACC
AseI 1503 ATTAAT AT^TAAT
2035 ATTAAT
AsnI 1503 ATTAAT AT^TAAT
2035 ATTAAT
Asp718I 1080 GGTACC G^GTACC
2272 GGTACC
BglII 588 AGATCT A^GATCT
1067 AGATCT
BsaAI 1385 CACGTG [CT]AC^GT[GA]
1906 TACGTG
Bsp1407I 1007 TGTACA T^GTACA
3625 TGTACA
BsrDI 419 GCAATG GCAATG(2/0)
5412 GCAATG
BsrGI 1007 TGTACA T^GTACA
3625 TGTACA
BstAPI 1759 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3598 GCACTGAGTGC
DraIII 149 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3599 CACTGAGTG
EcoRI 1531 GAATTC G^AATTC
3735 GAATTC
HaeII 34 GGCGCT [GA]GCGC^[CT]
2665 AGCGCT
HgaI 5336 GACGC GACGC(5/10)
4832 GCGTC
HincII 2181 GTTAAC GT[CT]^[GA]AC
4506 GTTAAC
HindII 2181 GTTAAC GT[CT]^[GA]AC
4506 GTTAAC
HpaI 2181 GTTAAC GTT^AAC
4506 GTTAAC
KpnI 1080 GGTACC GGTAC^C
2272 GGTACC
PpuMI 119 GGGACCT [GA]G^G[AT]CC[CT]
3005 GGGTCCT
PshBI 1503 ATTAAT AT^TAAT
2035 ATTAAT
PstI 815 CTGCAG CTGCA^G
919 CTGCAG
SmaI 329 CCCGGG CCC^GGG
2677 CCCGGG
SmlI 2637 CTCAAG C^T[CT][GA]AG
3985 CTTAAG
SspBI 1007 TGTACA T^GTACA
3625 TGTACA
SspI 1557 AATATT AAT^ATT
4295 AATATT
XmaCI 329 CCCGGG C^CCGGG
2677 CCCGGG
XmaI 329 CCCGGG C^CCGGG
2677 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BclI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII