##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BbrPI BciVI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspHI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DrdI
Eam1105I EcoRI HpaI KpnI KspI
MamI MluI MroI NheI NotI
NruI NspV PflFI PinAI PmaCI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfuI SgrAI Sse8387I
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 993 GACGTC GACGT^C
AflII 2271 CTTAAG C^TTAAG
AlwNI 1803 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 4341 AGCGCT AGC^GCT
Asp700I 4384 GAATGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 5263 TGCGCA TGC^GCA
BalI 4666 TGGCCA TGG^CCA
BamHI 509 GGATCC G^GATCC
BbvCI 235 GCTGAGG CCTCAGC(-5/-2)
BcgI 3405 GCAGACACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2271 CTTAAG C^TTAAG
BglII 2338 AGATCT A^GATCT
BsaAI 3618 TACGTA [CT]AC^GT[GA]
BsaI 1455 GAGACC GGTCTC(1/5)
BsiEI 2602 CGGCCG CG[GA][CT]^CG
BsmI 4470 GCATTC GAATGC(1/-1)
BssHII 126 GCGCGC G^CGCGC
BssSI 24 CACGAG CACGAG(-5/-1)
BstAPI 4936 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5057 GGTTACC G^GT[ACGT]ACC
BstPI 5057 GGTTACC G^GT[ACGT]ACC
BstXI 4844 CCACCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 2602 CGGCCG C^GGCCG
EclXI 2602 CGGCCG C^GGCCG
Eco47III 4341 AGCGCT AGC^GCT
Eco52I 2602 CGGCCG C^GGCCG
EcoO65I 5057 GGTTACC G^GT[ACGT]ACC
EcoRV 2079 GATATC GAT^ATC
EcoT22I 2755 ATGCAT ATGCA^T
FseI 2603 GGCCGGCC GGCCGG^CC
FspI 5263 TGCGCA TGC^GCA
HgaI 628 GCGTC GACGC(5/10)
HincII 1694 GTTGAC GT[CT]^[GA]AC
HindII 1694 GTTGAC GT[CT]^[GA]AC
HindIII 4827 AAGCTT A^AGCTT
MluNI 4666 TGGCCA TGG^CCA
MscI 4666 TGGCCA TGG^CCA
NcoI 3111 CCATGG C^CATGG
NsiI 2755 ATGCAT ATGCA^T
PacI 487 TTAATTAA TTAAT^TAA
PmeI 3748 GTTTAAAC GTTT^AAAC
PvuII 4743 CAGCTG CAG^CTG
SacI 829 GAGCTC GAGCT^C
ScaI 2131 AGTACT AGT^ACT
SfiI 603 GGCCCCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2609 CCCGGG CCC^GGG
SnaBI 3618 TACGTA TAC^GTA
SpeI 1373 ACTAGT A^CTAGT
StuI 677 AGGCCT AGG^CCT
XmaCI 2609 CCCGGG C^CCGGG
XmaI 2609 CCCGGG C^CCGGG
XmnI 4384 GAATGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 127 CGCG CG^CG
940 CGCG
AocI 540 CCTGAGG CC^T[ACGT]AGG
4750 CCTGAGG
ApaI 196 GGGCCC GGGCC^C
2455 GGGCCC
BbeI 49 GGCGCC GGCGC^C
184 GGCGCC
BclI 2574 TGATCA T^GATCA
3366 TGATCA
BglI 604 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4146 GCCAGGCAGGC
BsmAI 1455 GAGAC GTCTC(1/5)
363 GTCTC
BspMI 3591 ACCTGC ACCTGC(4/8)
2955 GCAGGT
BsrFI 609 GCCGGC [GA]^CCGG[CT]
2604 GCCGGC
BstUI 127 CGCG CG^CG
940 CGCG
Bsu36I 540 CCTGAGG CC^T[ACGT]AGG
4750 CCTGAGG
Cfr10I 609 GCCGGC [GA]^CCGG[CT]
2604 GCCGGC
DraIII 1309 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
2051 CACACAGTG
EarI 4483 CTCTTC CTCTTC(1/4)
5241 GAAGAG
Eco81I 540 CCTGAGG CC^T[ACGT]AGG
4750 CCTGAGG
EcoNI 1467 CCTGGTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3439 CCTTTAATAGG
FbaI 2574 TGATCA T^GATCA
3366 TGATCA
KasI 49 GGCGCC G^GCGCC
184 GGCGCC
Ksp632I 4483 CTCTTC CTCTTC(1/4)
5241 GAAGAG
MspA1I 82 CCGCTG C[AC]G^C[GT]G
4743 CAGCTG
MvnI 127 CGCG CG^CG
940 CGCG
NaeI 609 GCCGGC GCC^GGC
2604 GCCGGC
NarI 49 GGCGCC GG^CGCC
184 GGCGCC
NgoMIV 609 GCCGGC G^CCGGC
2604 GCCGGC
PaeR7I 703 CTCGAG C^TCGAG
4689 CTCGAG
PflMI 1212 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3761 CCATATGTTGG
SfoI 49 GGCGCC GGC^GCC
184 GGCGCC
SphI 1317 GCATGC GCATG^C
3598 GCATGC
SspI 3079 AATATT AAT^ATT
5395 AATATT
Van91I 1212 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3761 CCATATGTTGG
XhoI 703 CTCGAG C^TCGAG
4689 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
Bsp1407I BspLU11I BsrBI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspI MunI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII