##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AhdI Aor51HI AscI Asp718I AspEI
AspI AviII AvrII BbrPI BbvCI
BcgI BclI BglI BlnI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
CelII ClaI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FbaI FseI FspI
HgaI HpaI KpnI MfeI MluI
MroI MunI NaeI NcoI NgoMIV
NotI NruI PacI PflFI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII SacI SalI SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AcyI 197 GGCGCC G[GA]^CG[CT]C
AflII 4109 CTTAAG C^TTAAG
AgeI 401 ACCGGT A^CCGGT
Alw44I 4032 GTGCAC G^TGCAC
AlwNI 2513 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1701 CCTCAGG CC^T[ACGT]AGG
ApaI 237 GGGCCC GGGCC^C
ApaLI 4032 GTGCAC G^TGCAC
AseI 990 ATTAAT AT^TAAT
AsnI 990 ATTAAT AT^TAAT
Asp700I 1026 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1871 TGGCCA TGG^CCA
BamHI 1955 GGATCC G^GATCC
BanII 237 GGGCCC G[GA]GC[CT]^C
BbeI 197 GGCGCC GGCGC^C
BciVI 1442 GGATAC GTATCC(6/5)
BfrI 4109 CTTAAG C^TTAAG
BsaBI 1707 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 197 GGCGCC G[GA]^CG[CT]C
BsaI 1284 GAGACC GGTCTC(1/5)
BsaWI 401 ACCGGT [AT]^CCGG[AT]
BsgI 1784 GTGCAG GTGCAG(16/14)
Bsp1407I 4420 TGTACA T^GTACA
BsrBI 117 GAGCGG CCGCTC(-3/-3)
BsrFI 401 ACCGGT [GA]^CCGG[CT]
BsrGI 4420 TGTACA T^GTACA
BssSI 441 CACGAG CACGAG(-5/-1)
BstAPI 4147 GCATTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1701 CCTCAGG CC^T[ACGT]AGG
Cfr10I 401 ACCGGT [GA]^CCGG[CT]
CpoI 93 CGGTCCG CG^G[AT]CCG
DsaI 370 CCGCGG C^C[GA][CT]GG
EaeI 1871 TGGCCA [CT]^GGCC[GA]
Eco81I 1701 CCTCAGG CC^T[ACGT]AGG
EcoRI 2463 GAATTC G^AATTC
EcoT22I 2181 ATGCAT ATGCA^T
Hin1I 197 GGCGCC G[GA]^CG[CT]C
KasI 197 GGCGCC G^GCGCC
KspI 370 CCGCGG CCGC^GG
MamI 1707 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1871 TGGCCA TGG^CCA
MscI 1871 TGGCCA TGG^CCA
NarI 197 GGCGCC GG^CGCC
NheI 4143 GCTAGC G^CTAGC
NsiI 2181 ATGCAT ATGCA^T
PaeR7I 3995 CTCGAG C^TCGAG
PflMI 2427 CCATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 401 ACCGGT A^CCGGT
PpuMI 1247 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 990 ATTAAT AT^TAAT
PstI 3906 CTGCAG CTGCA^G
RsrII 93 CGGTCCG CG^G[AT]CCG
SacII 370 CCGCGG CCGC^GG
SapI 1026 GAAGAGC GCTCTTC(1/4)
SfoI 197 GGCGCC GGC^GCC
SphI 2179 GCATGC GCATG^C
SspBI 4420 TGTACA T^GTACA
StuI 457 AGGCCT AGG^CCT
Van91I 2427 CCATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2705 TCTAGA T^CTAGA
XcmI 1147 CCATGCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3995 CTCGAG C^TCGAG
XmnI 1026 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 544 ACATGT A^C[GA][CT]GT
2592 ACATGT
BanI 197 GGCGCC G^G[CT][GA]CC
830 GGTGCC
BbsI 2837 GTCTTC GAAGAC(2/6)
3745 GTCTTC
BpmI 453 CTCCAG CTGGAG(16/14)
560 CTCCAG
BpuAI 2837 GTCTTC GAAGAC(2/6)
3745 GTCTTC
BspHI 2056 TCATGA T^CATGA
3384 TCATGA
BspLU11I 544 ACATGT A^CATGT
2592 ACATGT
BsrDI 1539 CATTGC GCAATG(2/0)
3138 CATTGC
BstBI 493 TTCGAA TT^CGAA
1230 TTCGAA
DraII 236 GGGGCCC [GA]G^G[ACGT]CC[CT]
1247 GGGACCT
EcoO109I 236 GGGGCCC [GA]G^G[ACGT]CC[CT]
1247 GGGACCT
EcoT14I 1545 CCTTGG C^C[AT][AT]GG
3261 CCAAGG
HindIII 2353 AAGCTT A^AGCTT
3730 AAGCTT
MspA1I 370 CCGCGG C[AC]G^C[GT]G
379 CAGCGG
NspV 493 TTCGAA TT^CGAA
1230 TTCGAA
PleI 956 GACTC GAGTC(4/5)
1343 GAGTC
RcaI 2056 TCATGA T^CATGA
3384 TCATGA
SfaNI 3693 GATGC GCATC(5/9)
1589 GCATC
SfuI 493 TTCGAA TT^CGAA
1230 TTCGAA
StyI 1545 CCTTGG C^C[AT][AT]GG
3261 CCAAGG
Tsp45I 954 GTGAC ^GT[GC]AC
3293 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BbvI BcnI BfaI BglII BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII