##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI Asp718I AviII
AvrII BamHI BbvCI BcgI BciVI
BfrI BglII BlnI BsaBI BsiWI
Bsp1407I BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CpoI DrdI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoT22I FseI FspI KpnI MamI
MfeI MluI MunI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SapI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI XcmI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 2174 GGCGCC G[GA]^CG[CT]C
AhdI 1864 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2059 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1308 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1864 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2174 GGCGCC GGCGC^C
BbrPI 2779 CACGTG CAC^GTG
BclI 1167 TGATCA T^GATCA
BsaHI 2174 GGCGCC G[GA]^CG[CT]C
BsmBI 365 GAGACG CGTCTC(1/5)
BspDI 142 ATCGAT AT^CGAT
BsrDI 2281 GCAATG GCAATG(2/0)
BsrFI 978 GCCGGC [GA]^CCGG[CT]
BstXI 2415 CCACCGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 978 GCCGGC [GA]^CCGG[CT]
ClaI 142 ATCGAT AT^CGAT
EagI 2478 CGGCCG C^GGCCG
Eam1105I 1864 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2478 CGGCCG C^GGCCG
Eco52I 2478 CGGCCG C^GGCCG
EcoRV 2294 GATATC GAT^ATC
FbaI 1167 TGATCA T^GATCA
Hin1I 2174 GGCGCC G[GA]^CG[CT]C
KasI 2174 GGCGCC G^GCGCC
NaeI 978 GCCGGC GCC^GGC
NarI 2174 GGCGCC GG^CGCC
NgoMIV 978 GCCGGC G^CCGGC
NheI 2647 GCTAGC G^CTAGC
PflFI 4 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2779 CACGTG CAC^GTG
PmlI 2779 CACGTG CAC^GTG
SfiI 2696 GGCCCTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2174 GGCGCC GGC^GCC
Tth111I 4 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1708 TCTAGA T^CTAGA
XmnI 1308 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1401 GTCGAC GT^[AC][GT]AC
1558 GTCGAC
AccIII 639 TCCGGA T^CCGGA
828 TCCGGA
AfaI 2361 GTAC GT^AC
2871 GTAC
ApaI 69 GGGCCC GGGCC^C
2695 GGGCCC
AseI 3120 ATTAAT AT^TAAT
3212 ATTAAT
AsnI 3120 ATTAAT AT^TAAT
3212 ATTAAT
BglI 963 GCCTGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2697 GCCCTCGGGGC
BlpI 123 GCTCAGC GC^T[ACGT]AGC
559 GCTCAGC
Bpu1102I 123 GCTCAGC GC^T[ACGT]AGC
559 GCTCAGC
BsaAI 529 TACGTG [CT]AC^GT[GA]
2779 CACGTG
BsaI 2348 GAGACC GGTCTC(1/5)
2411 GAGACC
BsaWI 639 TCCGGA [AT]^CCGG[AT]
828 TCCGGA
BseAI 639 TCCGGA T^CCGGA
828 TCCGGA
BsgI 536 CTGCAC GTGCAG(16/14)
2300 GTGCAG
BsiEI 1748 CGGTCG CG[GA][CT]^CG
2478 CGGCCG
BsmI 3106 GCATTC GAATGC(1/-1)
3198 GCATTC
BspEI 639 TCCGGA T^CCGGA
828 TCCGGA
BspMI 401 ACCTGC ACCTGC(4/8)
1802 GCAGGT
CelII 123 GCTCAGC GC^T[ACGT]AGC
559 GCTCAGC
DraIII 2756 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2779 CACGTGGTG
EcoT14I 2448 CCATGG C^C[AT][AT]GG
2630 CCATGG
HindIII 790 AAGCTT A^AGCTT
1507 AAGCTT
HpaI 3097 GTTAAC GTT^AAC
3189 GTTAAC
KspI 923 CCGCGG CCGC^GG
2724 CCGCGG
MaeII 530 ACGT A^CGT
2780 ACGT
MroI 639 TCCGGA T^CCGGA
828 TCCGGA
NcoI 2448 CCATGG C^CATGG
2630 CCATGG
NspI 573 GCATGC [GA]CATG^[CT]
2108 GCATGC
PaeR7I 1987 CTCGAG C^TCGAG
2044 CTCGAG
PpuMI 806 AGGACCT [GA]G^G[AT]CC[CT]
2460 AGGACCC
PshBI 3120 ATTAAT AT^TAAT
3212 ATTAAT
PstI 403 CTGCAG CTGCA^G
2038 CTGCAG
PvuII 541 CAGCTG CAG^CTG
2188 CAGCTG
RsaI 2361 GTAC GT^AC
2871 GTAC
SacII 923 CCGCGG CCGC^GG
2724 CCGCGG
SalI 1401 GTCGAC G^TCGAC
1558 GTCGAC
SphI 573 GCATGC GCATG^C
2108 GCATGC
StuI 1019 AGGCCT AGG^CCT
1991 AGGCCT
StyI 2448 CCATGG C^C[AT][AT]GG
2630 CCATGG
XhoI 1987 CTCGAG C^TCGAG
2044 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI