##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AccIII      AclI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AspEI       AspI        AvaI        
AviII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BclI        BglII       BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BsrBI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     FbaI        FseI        
FspI        KasI        KspI        MamI        MflI        
MluI        MroI        NarI        NcoI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    StuI        SwaI        Tth111I     
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII        3044  GACGTC               GACGT^C
Acc65I        188  GGTACC               G^GTACC
AccII          42  CGCG                 CG^CG
AcyI         3044  GACGTC               G[GA]^CG[CT]C
AflII        2556  CTTAAG               C^TTAAG
AgeI          185  ACCGGT               A^CCGGT
AocI          294  CCTTAGG              CC^T[ACGT]AGG
ApaI         2370  GGGCCC               GGGCC^C
AseI         3162  ATTAAT               AT^TAAT
AsnI         3162  ATTAAT               AT^TAAT
Asp700I      1778  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       188  GGTACC               G^GTACC
AvrII         853  CCTAGG               C^CTAGG
BalI         2609  TGGCCA               TGG^CCA
BbsI         1179  GAAGAC               GAAGAC(2/6)
BcnI            2  CCCGG                CC^[GC]GG
BfrI         2556  CTTAAG               C^TTAAG
BglI         1935  GCCTCAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          853  CCTAGG               C^CTAGG
BpuAI        1179  GAAGAC               GAAGAC(2/6)
BsaHI        3044  GACGTC               G[GA]^CG[CT]C
BsaWI         185  ACCGGT               [AT]^CCGG[AT]
BspMI        1591  GCAGGT               ACCTGC(4/8)
BsrDI        2786  GCAATG               GCAATG(2/0)
BstUI          42  CGCG                 CG^CG
BstXI        1787  CCAAATTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        294  CCTTAGG              CC^T[ACGT]AGG
EaeI         2609  TGGCCA               [CT]^GGCC[GA]
Eco81I        294  CCTTAGG              CC^T[ACGT]AGG
EcoNI        1999  CCTGGGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2039  GAATTC               G^AATTC
FauI           40  CCCGC                CCCGC(4/6)
HgaI           43  GCGTC                GACGC(5/10)
Hin1I        3044  GACGTC               G[GA]^CG[CT]C
HindIII      2472  AAGCTT               A^AGCTT
KpnI          188  GGTACC               GGTAC^C
MluNI        2609  TGGCCA               TGG^CCA
MscI         2609  TGGCCA               TGG^CCA
MvnI           42  CGCG                 CG^CG
NaeI          114  GCCGGC               GCC^GGC
NciI            2  CCCGG                CC^[GC]GG
NgoMIV        114  GCCGGC               G^CCGGC
PinAI         185  ACCGGT               A^CCGGT
PleI         1402  GAGTC                GAGTC(4/5)
PpuMI        3636  AGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        3162  ATTAAT               AT^TAAT
PvuII          58  CAGCTG               CAG^CTG
SexAI         191  ACCTGGT              A^CC[AT]GGT
SfcI           25  CTACAG               C^T[GA][CT]AG
SphI         3392  GCATGC               GCATG^C
XmnI         1778  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI           23  GTCTAC               GT^[AC][GT]AC
             2947  GTAGAC              
AlwNI        1034  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1342  CAGCATCTG           
BciVI        1899  GGATAC               GTATCC(6/5)
              408  GTATCC              
BseRI        3273  CTCCTC               GAGGAG(10/8)
              100  GAGGAG              
BsgI          357  CTGCAC               GTGCAG(16/14)
             2524  GTGCAG              
BsmAI         353  GAGAC                GTCTC(1/5)
             3987  GTCTC               
BsmI         1135  GCATTC               GAATGC(1/-1)
             1913  GCATTC              
BsrFI         114  GCCGGC               [GA]^CCGG[CT]
              185  ACCGGT              
CfoI          243  GCGC                 GCG^C
             2755  GCGC                
Cfr10I        114  GCCGGC               [GA]^CCGG[CT]
              185  ACCGGT              
DraII        2370  GGGCCCC              [GA]G^G[ACGT]CC[CT]
             3636  AGGTCCC             
EcoO109I     2370  GGGCCCC              [GA]G^G[ACGT]CC[CT]
             3636  AGGTCCC             
EcoRV         334  GATATC               GAT^ATC
             3519  GATATC              
HaeII         242  AGCGCC               [GA]GCGC^[CT]
             2754  AGCGCC              
HaeIII       2371  GGCC                 GG^CC
             2610  GGCC                
HhaI          243  GCGC                 GCG^C
             2755  GCGC                
HinP1I        243  GCGC                 G^CGC
             2755  GCGC                
HpaI         1392  GTTAAC               GTT^AAC
             2517  GTTAAC              
MfeI         1251  CAATTG               C^AATTG
             1307  CAATTG              
MslI          171  CACAGGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3608  CATAAGTGTG          
MunI         1251  CAATTG               C^AATTG
             1307  CAATTG              
NdeI         3906  CATATG               CA^TATG
             3914  CATATG              
PflMI        1033  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3204  CCACATATTGG         
SacI          492  GAGCTC               GAGCT^C
             2453  GAGCTC              
Van91I       1033  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3204  CCACATATTGG         
XcmI          513  CCAAATGCTAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1551  CCAATGTAAACATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AspHI       AvaII       BanI        
BanII       BbvI        BfaI        BmyI        BpmI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    Bsp1407I    BspLU11I    BsrGI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        EarI        EcoRII      
EcoT14I     EcoT22I     Fnu4HI      FokI        HapII       
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NsiI        NspI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
SspBI       SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI