##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI Asp718I AviII AvrII BcgI
BlnI BsaI BseAI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI
BssHII CpoI DrdI EagI EclXI
Eco47III Eco52I FseI FspI HpaI
KpnI KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI PacI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacII
ScaI SfiI SgrAI SnaBI SspI
StuI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 4589 GACGTC GACGT^C
Alw44I 4407 GTGCAC G^TGCAC
ApaLI 4407 GTGCAC G^TGCAC
Asp700I 4315 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4738 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3465 GGCGCC GGCGC^C
BciVI 2414 GGATAC GTATCC(6/5)
BclI 3649 TGATCA T^GATCA
BglII 2055 AGATCT A^GATCT
BsaBI 3166 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2208 TCCGGT [AT]^CCGG[AT]
Bsp1407I 4428 TGTACA T^GTACA
BspDI 1442 ATCGAT AT^CGAT
BspLU11I 3909 ACATGT A^CATGT
BsrFI 1351 ACCGGC [GA]^CCGG[CT]
BsrGI 4428 TGTACA T^GTACA
Bst1107I 4819 GTATAC GTA^TAC
BstBI 1464 TTCGAA TT^CGAA
BstEII 4513 GGTGACC G^GT[ACGT]ACC
BstPI 4513 GGTGACC G^GT[ACGT]ACC
BstZ17I 4819 GTATAC GTA^TAC
Cfr10I 1351 ACCGGC [GA]^CCGG[CT]
ClaI 1442 ATCGAT AT^CGAT
EcoO65I 4513 GGTGACC G^GT[ACGT]ACC
EcoRV 1381 GATATC GAT^ATC
EcoT22I 5596 ATGCAT ATGCA^T
FbaI 3649 TGATCA T^GATCA
KasI 3465 GGCGCC G^GCGCC
MamI 3166 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3465 GGCGCC GG^CGCC
NsiI 5596 ATGCAT ATGCA^T
NspV 1464 TTCGAA TT^CGAA
PaeR7I 89 CTCGAG C^TCGAG
PflFI 4738 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 526 GTCGAC G^TCGAC
SapI 4255 GCTCTTC GCTCTTC(1/4)
SexAI 3317 ACCAGGT A^CC[AT]GGT
SfoI 3465 GGCGCC GGC^GCC
SfuI 1464 TTCGAA TT^CGAA
SmaI 3456 CCCGGG CCC^GGG
SpeI 2201 ACTAGT A^CTAGT
SphI 1634 GCATGC GCATG^C
SspBI 4428 TGTACA T^GTACA
Tth111I 4738 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1069 TCTAGA T^CTAGA
XcmI 1197 CCACAGAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 89 CTCGAG C^TCGAG
XmaCI 3456 CCCGGG C^CCGGG
XmaI 3456 CCCGGG C^CCGGG
XmnI 4315 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 4 CGCG CG^CG
3640 CGCG
AcyI 3465 GGCGCC G[GA]^CG[CT]C
4589 GACGTC
AflII 164 CTTAAG C^TTAAG
4792 CTTAAG
AhdI 1670 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3894 GACTGGATGTC
AseI 413 ATTAAT AT^TAAT
4957 ATTAAT
AsnI 413 ATTAAT AT^TAAT
4957 ATTAAT
AspEI 1670 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3894 GACTGGATGTC
BamHI 114 GGATCC G^GATCC
476 GGATCC
BbrPI 1310 CACGTG CAC^GTG
1672 CACGTG
BfrI 164 CTTAAG C^TTAAG
4792 CTTAAG
BglI 1478 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1733 GCCGACATGGC
BsaHI 3465 GGCGCC G[GA]^CG[CT]C
4589 GACGTC
BsmI 2598 GCATTC GAATGC(1/-1)
3218 GCATTC
BssSI 4446 CACGAG CACGAG(-5/-1)
1871 CTCGTG
BstUI 4 CGCG CG^CG
3640 CGCG
CfoI 1487 GCGC GCG^C
3466 GCGC
DraIII 3978 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4247 CACAACGTG
Eam1105I 1670 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3894 GACTGGATGTC
EcoRI 1272 GAATTC G^AATTC
3122 GAATTC
HaeII 1486 GGCGCT [GA]GCGC^[CT]
3465 GGCGCC
HhaI 1487 GCGC GCG^C
3466 GCGC
Hin1I 3465 GGCGCC G[GA]^CG[CT]C
4589 GACGTC
HinP1I 1487 GCGC G^CGC
3466 GCGC
HincII 526 GTCGAC GT[CT]^[GA]AC
698 GTCAAC
HindII 526 GTCGAC GT[CT]^[GA]AC
698 GTCAAC
MvnI 4 CGCG CG^CG
3640 CGCG
NspI 1634 GCATGC [GA]CATG^[CT]
3909 ACATGT
PmaCI 1310 CACGTG CAC^GTG
1672 CACGTG
PmlI 1310 CACGTG CAC^GTG
1672 CACGTG
PshAI 2624 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3515 GACCTGGGTC
PshBI 413 ATTAAT AT^TAAT
4957 ATTAAT
SacI 1868 GAGCTC GAGCT^C
4643 GAGCTC
SbfI 606 CCTGCAGG CCTGCA^GG
1153 CCTGCAGG
Sse8387I 606 CCTGCAGG CCTGCA^GG
1153 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII