##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspI        AvaII       AviII       BamHI       
BbrPI       BcgI        BfrI        BlpI        Bpu1102I    
BsaAI       BsaBI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraII       
DraIII      EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
FseI        FspI        HincII      HindII      HpaI        
KpnI        MaeII       MamI        MluI        MroI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SwaI        
Tth111I     XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         3047  GTCTAC               GT^[AC][GT]AC
AcyI           19  GGCGCC               G[GA]^CG[CT]C
AhdI          654  GACAAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         654  GACAAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         2630  CCCGGG               C^[CT]CG[GA]G
AvrII        2984  CCTAGG               C^CTAGG
BbeI           19  GGCGCC               GGCGC^C
BbvCI        2722  GCTGAGG              CCTCAGC(-5/-2)
BciVI        3153  GTATCC               GTATCC(6/5)
BglII         449  AGATCT               A^GATCT
BlnI         2984  CCTAGG               C^CTAGG
BsaHI          19  GGCGCC               G[GA]^CG[CT]C
BsaI          942  GGTCTC               GGTCTC(1/5)
BsaWI         543  TCCGGT               [AT]^CCGG[AT]
BsmBI        2651  CGTCTC               CGTCTC(1/5)
BsmFI        1428  GGGAC                GGGAC(10/14)
BsoBI        2630  CCCGGG               C^[CT]CG[GA]G
BspHI         680  TCATGA               T^CATGA
BssHII        138  GCGCGC               G^CGCGC
DrdI         1488  GACTGAAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      654  GACAAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1504  GAAGAG               CTCTTC(1/4)
EcoRV        2102  GATATC               GAT^ATC
EcoT22I       393  ATGCAT               ATGCA^T
FauI           73  GCGGG                CCCGC(4/6)
HaeII          19  GGCGCC               [GA]GCGC^[CT]
Hin1I          19  GGCGCC               G[GA]^CG[CT]C
KasI           19  GGCGCC               G^GCGCC
Ksp632I      1504  GAAGAG               CTCTTC(1/4)
MfeI         3136  CAATTG               C^AATTG
MunI         3136  CAATTG               C^AATTG
NarI           19  GGCGCC               GG^CGCC
NsiI          393  ATGCAT               ATGCA^T
PflMI        2426  CCATGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         2761  CTGCAG               CTGCA^G
RcaI          680  TCATGA               T^CATGA
SacI         2608  GAGCTC               GAGCT^C
SfiI         2012  GGCCACCGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           19  GGCGCC               GGC^GCC
SmaI         2630  CCCGGG               CCC^GGG
SphI         2693  GCATGC               GCATG^C
SspI         2946  AATATT               AAT^ATT
StuI         2585  AGGCCT               AGG^CCT
Van91I       2426  CCATGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1658  TCTAGA               T^CTAGA
XmaCI        2630  CCCGGG               C^CCGGG
XmaI         2630  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AseI         2311  ATTAAT               AT^TAAT
             2991  ATTAAT              
AsnI         2311  ATTAAT               AT^TAAT
             2991  ATTAAT              
BalI         1937  TGGCCA               TGG^CCA
             2372  TGGCCA              
BanI           19  GGCGCC               G^G[CT][GA]CC
             2884  GGCACC              
BanII          27  GAGCCC               G[GA]GC[CT]^C
             2608  GAGCTC              
BclI          648  TGATCA               T^GATCA
             1401  TGATCA              
BglI          142  GCCCGAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2013  GCCACCGCGGC         
BsmI         2247  GAATGC               GAATGC(1/-1)
             1066  GCATTC              
BstYI         449  AGATCT               [GA]^GATC[CT]
             1167  AGATCC              
FbaI          648  TGATCA               T^GATCA
             1401  TGATCA              
HindIII       204  AAGCTT               A^AGCTT
             1678  AAGCTT              
KspI           71  CCGCGG               CCGC^GG
             2017  CCGCGG              
MflI          449  AGATCT               [GA]^GATC[CT]
             1167  AGATCC              
MluNI        1937  TGGCCA               TGG^CCA
             2372  TGGCCA              
MscI         1937  TGGCCA               TGG^CCA
             2372  TGGCCA              
NcoI          111  CCATGG               C^CATGG
             1368  CCATGG              
NlaIV          19  GGCGCC               GG[ACGT]^[ACGT]CC
             2884  GGCACC              
NspI         1248  ACATGC               [GA]CATG^[CT]
             2693  GCATGC              
PshBI        2311  ATTAAT               AT^TAAT
             2991  ATTAAT              
SacII          71  CCGCGG               CCGC^GG
             2017  CCGCGG              
XhoII         449  AGATCT               [GA]^GATC[CT]
             1167  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    Bsp1407I    BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DsaI        EaeI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I