##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BciVI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BspEI BsrDI BsrFI BssSI
Bst1107I BstAPI BstBI BstZ17I Cfr10I
CpoI EagI EclXI Eco47III Eco52I
EcoRI EcoT22I FseI FspI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 1442 CGCG CG^CG
AcsI 2754 GAATTT [GA]^AATT[CT]
AflII 3532 CTTAAG C^TTAAG
ApoI 2754 GAATTT [GA]^AATT[CT]
BamHI 529 GGATCC G^GATCC
BbeI 1227 GGCGCC GGCGC^C
BbrPI 1662 CACGTG CAC^GTG
BbvCI 1255 CCTCAGC CCTCAGC(-5/-2)
BcgI 880 GCACGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3532 CTTAAG C^TTAAG
BsaAI 1662 CACGTG [CT]AC^GT[GA]
BsmBI 2130 GAGACG CGTCTC(1/5)
BsmI 2403 GCATTC GAATGC(1/-1)
Bsp1407I 262 TGTACA T^GTACA
BspDI 1676 ATCGAT AT^CGAT
BspHI 2764 TCATGA T^CATGA
BspLU11I 3249 ACATGT A^CATGT
BsrGI 262 TGTACA T^GTACA
BssHII 1441 GCGCGC G^CGCGC
BstEII 539 GGTGACC G^GT[ACGT]ACC
BstPI 539 GGTGACC G^GT[ACGT]ACC
BstUI 1442 CGCG CG^CG
ClaI 1676 ATCGAT AT^CGAT
DraI 696 TTTAAA TTT^AAA
DraIII 2681 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2659 GACAGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 476 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 539 GGTGACC G^GT[ACGT]ACC
EcoRV 3702 GATATC GAT^ATC
HincII 570 GTTAAC GT[CT]^[GA]AC
HindII 570 GTTAAC GT[CT]^[GA]AC
HpaI 570 GTTAAC GTT^AAC
KasI 1227 GGCGCC G^GCGCC
MvnI 1442 CGCG CG^CG
NarI 1227 GGCGCC GG^CGCC
PmaCI 1662 CACGTG CAC^GTG
PmlI 1662 CACGTG CAC^GTG
PshAI 3053 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2764 TCATGA T^CATGA
SacI 1641 GAGCTC GAGCT^C
SapI 1589 GCTCTTC GCTCTTC(1/4)
SbfI 476 CCTGCAGG CCTGCA^GG
SfoI 1227 GGCGCC GGC^GCC
Sse8387I 476 CCTGCAGG CCTGCA^GG
SspBI 262 TGTACA T^GTACA
XcmI 2985 CCAGATGGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 306 GACGCC G[GA]^CG[CT]C
1227 GGCGCC
AflIII 556 ACACGT A^C[GA][CT]GT
3249 ACATGT
AhdI 219 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
542 GACCAAGCGTC
AspEI 219 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
542 GACCAAGCGTC
AspHI 1641 GAGCTC G[AT]GC[AT]^C
2021 GAGCAC
BbsI 239 GAAGAC GAAGAC(2/6)
516 GAAGAC
BclI 107 TGATCA T^GATCA
665 TGATCA
BglI 2113 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2513 GCCCAAGGGGC
BlpI 2018 GCTGAGC GC^T[ACGT]AGC
3333 GCTCAGC
Bpu1102I 2018 GCTGAGC GC^T[ACGT]AGC
3333 GCTCAGC
BpuAI 239 GAAGAC GAAGAC(2/6)
516 GAAGAC
BsaHI 306 GACGCC G[GA]^CG[CT]C
1227 GGCGCC
BsiHKAI 1641 GAGCTC G[AT]GC[AT]^C
2021 GAGCAC
BsrBI 1013 CCGCTC CCGCTC(-3/-3)
1587 CCGCTC
BstYI 166 AGATCC [GA]^GATC[CT]
529 GGATCC
CelII 2018 GCTGAGC GC^T[ACGT]AGC
3333 GCTCAGC
Eam1105I 219 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
542 GACCAAGCGTC
FbaI 107 TGATCA T^GATCA
665 TGATCA
HaeII 191 GGCGCT [GA]GCGC^[CT]
1227 GGCGCC
Hin1I 306 GACGCC G[GA]^CG[CT]C
1227 GGCGCC
HindIII 435 AAGCTT A^AGCTT
3308 AAGCTT
MflI 166 AGATCC [GA]^GATC[CT]
529 GGATCC
NdeI 2306 CATATG CA^TATG
3055 CATATG
PpuMI 998 AGGACCT [GA]G^G[AT]CC[CT]
2268 AGGACCT
SmaI 769 CCCGGG CCC^GGG
2351 CCCGGG
StuI 991 AGGCCT AGG^CCT
3025 AGGCCT
XbaI 1475 TCTAGA T^CTAGA
3629 TCTAGA
XhoII 166 AGATCC [GA]^GATC[CT]
529 GGATCC
XmaCI 769 CCCGGG C^CCGGG
2351 CCCGGG
XmaI 769 CCCGGG C^CCGGG
2351 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I