##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AviII BbeI BbrPI BbvCI BglI
BglII BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspMI BssHII BstAPI BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I FseI
FspI HpaI KasI KpnI KspI
MamI MluI MroI NarI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 3037 GACGTC GACGT^C
AccI 434 GTATAC GT^[AC][GT]AC
AflII 3710 CTTAAG C^TTAAG
Alw44I 3982 GTGCAC G^TGCAC
AlwNI 951 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3982 GTGCAC G^TGCAC
AseI 1764 ATTAAT AT^TAAT
AsnI 1764 ATTAAT AT^TAAT
AspI 126 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3549 GGATCC G^GATCC
BcgI 57 CGAGACACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 464 TGATCA T^GATCA
BfrI 3710 CTTAAG C^TTAAG
BsaAI 286 CACGTA [CT]AC^GT[GA]
BsaI 313 GAGACC GGTCTC(1/5)
BsmBI 2676 CGTCTC CGTCTC(1/5)
Bsp1407I 365 TGTACA T^GTACA
BspLU11I 2318 ACATGT A^CATGT
BsrGI 365 TGTACA T^GTACA
Bst1107I 434 GTATAC GTA^TAC
BstZ17I 434 GTATAC GTA^TAC
DsaI 1702 CCATGG C^C[GA][CT]GG
EcoRI 1620 GAATTC G^AATTC
EcoRV 4058 GATATC GAT^ATC
FbaI 464 TGATCA T^GATCA
HincII 1677 GTTGAC GT[CT]^[GA]AC
HindII 1677 GTTGAC GT[CT]^[GA]AC
MfeI 3694 CAATTG C^AATTG
MunI 3694 CAATTG C^AATTG
NaeI 3294 GCCGGC GCC^GGC
NcoI 1702 CCATGG C^CATGG
NgoMIV 3294 GCCGGC G^CCGGC
NspI 2318 ACATGT [GA]CATG^[CT]
PflFI 126 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1829 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3319 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1764 ATTAAT AT^TAAT
SacI 628 GAGCTC GAGCT^C
SspBI 365 TGTACA T^GTACA
StuI 3562 AGGCCT AGG^CCT
Tth111I 126 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 806 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 91 CGCG CG^CG
1659 CGCG
AcyI 1511 GGCGTC G[GA]^CG[CT]C
3037 GACGTC
AflIII 1912 ACGTGT A^C[GA][CT]GT
2318 ACATGT
ApaI 2260 GGGCCC GGGCC^C
2719 GGGCCC
AvrII 1484 CCTAGG C^CTAGG
1694 CCTAGG
BalI 1984 TGGCCA TGG^CCA
3515 TGGCCA
BciVI 344 GGATAC GTATCC(6/5)
1307 GTATCC
BlnI 1484 CCTAGG C^CTAGG
1694 CCTAGG
BsaHI 1511 GGCGTC G[GA]^CG[CT]C
3037 GACGTC
BsaWI 158 ACCGGA [AT]^CCGG[AT]
3375 TCCGGT
BsrBI 46 GAGCGG CCGCTC(-3/-3)
2598 GAGCGG
BsrDI 652 CATTGC GCAATG(2/0)
702 CATTGC
BsrFI 36 ACCGGC [GA]^CCGG[CT]
3294 GCCGGC
BssSI 2083 CTCGTG CACGAG(-5/-1)
3795 CTCGTG
BstUI 91 CGCG CG^CG
1659 CGCG
Cfr10I 36 ACCGGC [GA]^CCGG[CT]
3294 GCCGGC
DraIII 1876 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2326 CACCCAGTG
EcoNI 500 CCTACTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1484 CCTAGGGGAGG
EcoT22I 967 ATGCAT ATGCA^T
1162 ATGCAT
HaeII 227 GGCGCT [GA]GCGC^[CT]
2181 AGCGCC
HgaI 1512 GCGTC GACGC(5/10)
1964 GCGTC
Hin1I 1511 GGCGTC G[GA]^CG[CT]C
3037 GACGTC
MluNI 1984 TGGCCA TGG^CCA
3515 TGGCCA
MscI 1984 TGGCCA TGG^CCA
3515 TGGCCA
MvnI 91 CGCG CG^CG
1659 CGCG
NdeI 212 CATATG CA^TATG
3838 CATATG
NheI 1019 GCTAGC G^CTAGC
1898 GCTAGC
NsiI 967 ATGCAT ATGCA^T
1162 ATGCAT
PstI 3850 CTGCAG CTGCA^G
4119 CTGCAG
SmaI 2257 CCCGGG CCC^GGG
2572 CCCGGG
SmlI 504 CTTGAG C^T[CT][GA]AG
3710 CTTAAG
XcmI 1466 CCATTTTAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3272 CCAGAACAAGTCTGG
XmaCI 2257 CCCGGG C^CCGGG
2572 CCCGGG
XmaI 2257 CCCGGG C^CCGGG
2572 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI