##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BbrPI       BcgI        
BciVI       BlnI        BsaAI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspEI       BssHII      
BssSI       BstBI       BstEII      BstPI       DraI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        KpnI        MluI        MroI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
SalI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         3869  GTATAC               GT^[AC][GT]AC
Alw44I       2201  GTGCAC               G^TGCAC
ApaLI        2201  GTGCAC               G^TGCAC
Asp700I      3255  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1494  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         4648  TGGCCA               TGG^CCA
BbeI           43  GGCGCC               GGCGC^C
BbvCI         867  GCTGAGG              CCTCAGC(-5/-2)
BclI          182  TGATCA               T^GATCA
BsgI         3307  GTGCAG               GTGCAG(16/14)
BsmI         4621  GAATGC               GAATGC(1/-1)
BspDI         989  ATCGAT               AT^CGAT
BspHI        2320  TCATGA               T^CATGA
BsrBI         408  GAGCGG               CCGCTC(-3/-3)
BsrDI        2361  CATTGC               GCAATG(2/0)
Bst1107I     3869  GTATAC               GTA^TAC
BstAPI       4469  GCACAAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      3869  GTATAC               GTA^TAC
ClaI          989  ATCGAT               AT^CGAT
CpoI         2593  CGGTCCG              CG^G[AT]CCG
DrdI         4424  GACATGGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1727  GAATTC               G^AATTC
FbaI          182  TGATCA               T^GATCA
HincII       3230  GTCAAC               GT[CT]^[GA]AC
HindII       3230  GTCAAC               GT[CT]^[GA]AC
KasI           43  GGCGCC               G^GCGCC
KspI           92  CCGCGG               CCGC^GG
MfeI          886  CAATTG               C^AATTG
MluNI        4648  TGGCCA               TGG^CCA
MscI         4648  TGGCCA               TGG^CCA
MunI          886  CAATTG               C^AATTG
NaeI          505  GCCGGC               GCC^GGC
NarI           43  GGCGCC               GG^CGCC
NgoMIV        505  GCCGGC               G^CCGGC
PflFI        1494  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1546  CCAGCTATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         2320  TCATGA               T^CATGA
RsrII        2593  CGGTCCG              CG^G[AT]CCG
SacI         3833  GAGCTC               GAGCT^C
SacII          92  CCGCGG               CCGC^GG
SapI         1625  GAAGAGC              GCTCTTC(1/4)
SbfI         4374  CCTGCAGG             CCTGCA^GG
ScaI         2404  AGTACT               AGT^ACT
SfoI           43  GGCGCC               GGC^GCC
Sse8387I     4374  CCTGCAGG             CCTGCA^GG
SspI         4108  AATATT               AAT^ATT
Tth111I      1494  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1546  CCAGCTATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          785  TCTAGA               T^CTAGA
XmnI         3255  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI           43  GGCGCC               G[GA]^CG[CT]C
              401  GGCGTC              
AflII         807  CTTAAG               C^TTAAG
             2144  CTTAAG              
AflIII       2416  ACATGT               A^C[GA][CT]GT
             3051  ACATGT              
ApaI          540  GGGCCC               GGGCC^C
              665  GGGCCC              
BfrI          807  CTTAAG               C^TTAAG
             2144  CTTAAG              
BglII        1411  AGATCT               A^GATCT
             2306  AGATCT              
BlpI           71  GCTAAGC              GC^T[ACGT]AGC
             3651  GCTCAGC             
Bpu1102I       71  GCTAAGC              GC^T[ACGT]AGC
             3651  GCTCAGC             
BsaBI        2301  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3332  GATGGAGATC          
BsaHI          43  GGCGCC               G[GA]^CG[CT]C
              401  GGCGTC              
BsaI          934  GAGACC               GGTCTC(1/5)
             3272  GGTCTC              
Bsp1407I     1519  TGTACA               T^GTACA
             3579  TGTACA              
BspLU11I     2416  ACATGT               A^CATGT
             3051  ACATGT              
BsrFI         505  GCCGGC               [GA]^CCGG[CT]
             2370  ACCGGC              
BsrGI        1519  TGTACA               T^GTACA
             3579  TGTACA              
BstXI        1690  CCAGAGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3291  CCATTGATATGG        
CelII          71  GCTAAGC              GC^T[ACGT]AGC
             3651  GCTCAGC             
Cfr10I        505  GCCGGC               [GA]^CCGG[CT]
             2370  ACCGGC              
EaeI          507  CGGCCA               [CT]^GGCC[GA]
             4648  TGGCCA              
Hin1I          43  GGCGCC               G[GA]^CG[CT]C
              401  GGCGTC              
MamI         2301  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3332  GATGGAGATC          
NcoI          397  CCATGG               C^CATGG
             1055  CCATGG              
SspBI        1519  TGTACA               T^GTACA
             3579  TGTACA              
StuI         1702  AGGCCT               AGG^CCT
             3686  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        Aor51HI     ApoI        
AspHI       AvaI        AvaII       BamHI       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EarI        
Eco47III    Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       XmaCI       XmaI