##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbeI BbrPI BfrI
BlnI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI BstAPI BstEII BstPI
ClaI CpoI EcoO65I FauI FseI
FspI HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacII SalI ScaI SfiI SfoI
SgrAI SnaBI SpeI SphI SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AclI 516 AACGTT AA^CGTT
AcyI 3445 GGCGTC G[GA]^CG[CT]C
AflIII 3956 ACACGT A^C[GA][CT]GT
Alw44I 2398 GTGCAC G^TGCAC
Aor51HI 1001 AGCGCT AGC^GCT
ApaLI 2398 GTGCAC G^TGCAC
AspI 1075 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3185 TGGCCA TGG^CCA
BcgI 126 CGAAGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3330 GTATCC GTATCC(6/5)
BclI 1449 TGATCA T^GATCA
BglI 1180 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 468 AGATCT A^GATCT
BlpI 1559 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1559 GCTCAGC GC^T[ACGT]AGC
BsaAI 2788 TACGTG [CT]AC^GT[GA]
BsaHI 3445 GGCGTC G[GA]^CG[CT]C
BsaWI 857 ACCGGA [AT]^CCGG[AT]
BsiEI 3614 CGGCCG CG[GA][CT]^CG
BsmBI 122 GAGACG CGTCTC(1/5)
BspMI 3716 ACCTGC ACCTGC(4/8)
BsrFI 3612 ACCGGC [GA]^CCGG[CT]
Bst1107I 2937 GTATAC GTA^TAC
BstBI 3209 TTCGAA TT^CGAA
BstXI 2744 CCATTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2937 GTATAC GTA^TAC
CelII 1559 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3612 ACCGGC [GA]^CCGG[CT]
DraIII 1823 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 381 GACGATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3614 CGGCCG C^GGCCG
EclXI 3614 CGGCCG C^GGCCG
Eco47III 1001 AGCGCT AGC^GCT
Eco52I 3614 CGGCCG C^GGCCG
EcoRI 3806 GAATTC G^AATTC
EcoRV 832 GATATC GAT^ATC
EcoT22I 2953 ATGCAT ATGCA^T
FbaI 1449 TGATCA T^GATCA
HaeII 1001 AGCGCT [GA]GCGC^[CT]
Hin1I 3445 GGCGTC G[GA]^CG[CT]C
MfeI 3865 CAATTG C^AATTG
MluNI 3185 TGGCCA TGG^CCA
MscI 3185 TGGCCA TGG^CCA
MunI 3865 CAATTG C^AATTG
NcoI 3441 CCATGG C^CATGG
NsiI 2953 ATGCAT ATGCA^T
NspV 3209 TTCGAA TT^CGAA
PflFI 1075 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 516 AACGTT AA^CGTT
SbfI 4003 CCTGCAGG CCTGCA^GG
SexAI 3400 ACCTGGT A^CC[AT]GGT
SfuI 3209 TTCGAA TT^CGAA
SmaI 660 CCCGGG CCC^GGG
Sse8387I 4003 CCTGCAGG CCTGCA^GG
SspI 4168 AATATT AAT^ATT
Tth111I 1075 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2151 TCTAGA T^CTAGA
XmaCI 660 CCCGGG C^CCGGG
XmaI 660 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2937 GTATAC GT^[AC][GT]AC
3915 GTAGAC
AccII 2614 CGCG CG^CG
3625 CGCG
AhdI 3397 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3611 GACCGGCCGTC
AlwNI 639 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1906 CAGAAACTG
AocI 3489 CCTGAGG CC^T[ACGT]AGG
3786 CCTCAGG
ApaI 1126 GGGCCC GGGCC^C
2976 GGGCCC
Asp700I 2698 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3795 GAAGCTCTTC
AspEI 3397 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3611 GACCGGCCGTC
AvaI 660 CCCGGG C^[CT]CG[GA]G
2840 CCCGAG
BbvCI 2053 GCTGAGG CCTCAGC(-5/-2)
3856 GCTGAGG
BsaI 1936 GAGACC GGTCTC(1/5)
3609 GAGACC
BsoBI 660 CCCGGG C^[CT]CG[GA]G
2840 CCCGAG
BspHI 3633 TCATGA T^CATGA
4054 TCATGA
BsrDI 1768 CATTGC GCAATG(2/0)
2956 CATTGC
BstUI 2614 CGCG CG^CG
3625 CGCG
Bsu36I 3489 CCTGAGG CC^T[ACGT]AGG
3786 CCTCAGG
DraI 1008 TTTAAA TTT^AAA
4076 TTTAAA
DsaI 3419 CCACGG C^C[GA][CT]GG
3441 CCATGG
EaeI 3185 TGGCCA [CT]^GGCC[GA]
3614 CGGCCG
Eam1105I 3397 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3611 GACCGGCCGTC
Eco81I 3489 CCTGAGG CC^T[ACGT]AGG
3786 CCTCAGG
MvnI 2614 CGCG CG^CG
3625 CGCG
NspI 920 ACATGC [GA]CATG^[CT]
2951 ACATGC
PpuMI 291 GGGACCT [GA]G^G[AT]CC[CT]
3782 AGGACCT
RcaI 3633 TCATGA T^CATGA
4054 TCATGA
StuI 760 AGGCCT AGG^CCT
2057 AGGCCT
XcmI 1130 CCACCCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2231 CCAGATTTCTTGTGG
XmnI 2698 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3795 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII