##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI ApaI AscI AseI
AsnI Asp700I AspEI BamHI BbeI
BciVI BclI BfrI BsaHI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BssHII BssSI BstBI ClaI CpoI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI Hin1I HindIII KasI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 1635 GTGCAC G^TGCAC
Aor51HI 620 AGCGCT AGC^GCT
ApaLI 1635 GTGCAC G^TGCAC
AspI 1746 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1700 TGCGCA TGC^GCA
AvrII 1647 CCTAGG C^CTAGG
BbrPI 1704 CACGTG CAC^GTG
BbvCI 3308 CCTCAGC CCTCAGC(-5/-2)
BglII 2039 AGATCT A^GATCT
BlnI 1647 CCTAGG C^CTAGG
BlpI 451 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 451 GCTGAGC GC^T[ACGT]AGC
BsmI 4431 GCATTC GAATGC(1/-1)
Bsp1407I 3585 TGTACA T^GTACA
BsrDI 1041 CATTGC GCAATG(2/0)
BsrGI 3585 TGTACA T^GTACA
Bst1107I 3922 GTATAC GTA^TAC
BstZ17I 3922 GTATAC GTA^TAC
CelII 451 GCTGAGC GC^T[ACGT]AGC
Eco47III 620 AGCGCT AGC^GCT
FspI 1700 TGCGCA TGC^GCA
HpaI 4567 GTTAAC GTT^AAC
KspI 965 CCGCGG CCGC^GG
NaeI 1785 GCCGGC GCC^GGC
NgoMIV 1785 GCCGGC G^CCGGC
PflFI 1746 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1704 CACGTG CAC^GTG
PmlI 1704 CACGTG CAC^GTG
SacII 965 CCGCGG CCGC^GG
ScaI 3856 AGTACT AGT^ACT
SmlI 3017 CTTGAG C^T[CT][GA]AG
SspBI 3585 TGTACA T^GTACA
Tth111I 1746 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 79 GGTACC G^GTACC
2279 GGTACC
AocI 1668 CCTCAGG CC^T[ACGT]AGG
4536 CCTTAGG
Asp718I 79 GGTACC G^GTACC
2279 GGTACC
AvaI 1512 CTCGGG C^[CT]CG[GA]G
2507 CTCGGG
BcgI 1709 GCATGGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1802 GCACCACGTTCG
BsaAI 1704 CACGTG [CT]AC^GT[GA]
1876 TACGTG
BsaBI 1446 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2493 GATCCCTATC
BsaWI 195 TCCGGT [AT]^CCGG[AT]
434 ACCGGA
BsoBI 1512 CTCGGG C^[CT]CG[GA]G
2507 CTCGGG
BspLU11I 2585 ACATGT A^CATGT
4289 ACATGT
BspMI 2360 ACCTGC ACCTGC(4/8)
2531 ACCTGC
BsrBI 1404 CCGCTC CCGCTC(-3/-3)
1726 GAGCGG
BstAPI 1935 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2520 GCAGCGCATGC
Bsu36I 1668 CCTCAGG CC^T[ACGT]AGG
4536 CCTTAGG
DraIII 529 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3163 CACACAGTG
DrdI 271 GACAACCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
433 GACCGGATTGTC
EagI 460 CGGCCG C^GGCCG
1577 CGGCCG
EclXI 460 CGGCCG C^GGCCG
1577 CGGCCG
Eco52I 460 CGGCCG C^GGCCG
1577 CGGCCG
Eco81I 1668 CCTCAGG CC^T[ACGT]AGG
4536 CCTTAGG
EcoNI 586 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4129 CCTTCAACAGG
KpnI 79 GGTACC GGTAC^C
2279 GGTACC
MamI 1446 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2493 GATCCCTATC
PflMI 528 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
712 CCAGAGGTTGG
SapI 1764 GCTCTTC GCTCTTC(1/4)
3487 GCTCTTC
TaqI 1460 TCGA T^CGA
2291 TCGA
TthHB8I 1460 TCGA T^CGA
2291 TCGA
Van91I 528 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
712 CCAGAGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SphI
SspI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII