##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AspI AviII BseAI BsiWI BsmBI
BspEI BssHII BstBI BstEII BstPI
CpoI DraIII Eam1105I Eco47III EcoNI
EcoO65I EcoRI FseI FspI HpaI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NspV PflFI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Tth111I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2991 GACGTC GACGT^C
Acc65I 5484 GGTACC G^GTACC
AccI 5841 GTATAC GT^[AC][GT]AC
AocI 2285 CCTGAGG CC^T[ACGT]AGG
Asp718I 5484 GGTACC G^GTACC
BamHI 825 GGATCC G^GATCC
BbeI 265 GGCGCC GGCGC^C
BcgI 3196 GCACACAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2212 GGATAC GTATCC(6/5)
BlpI 3782 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3782 GCTCAGC GC^T[ACGT]AGC
BsaBI 2844 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4113 ACCGGA [AT]^CCGG[AT]
BspDI 2845 ATCGAT AT^CGAT
BsrDI 1733 GCAATG GCAATG(2/0)
BsrFI 99 GCCGGC [GA]^CCGG[CT]
Bst1107I 5841 GTATAC GTA^TAC
BstXI 1283 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5841 GTATAC GTA^TAC
Bsu36I 2285 CCTGAGG CC^T[ACGT]AGG
CelII 3782 GCTCAGC GC^T[ACGT]AGC
Cfr10I 99 GCCGGC [GA]^CCGG[CT]
ClaI 2845 ATCGAT AT^CGAT
DrdI 4034 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2285 CCTGAGG CC^T[ACGT]AGG
HincII 4602 GTTGAC GT[CT]^[GA]AC
HindII 4602 GTTGAC GT[CT]^[GA]AC
KasI 265 GGCGCC G^GCGCC
KpnI 5484 GGTACC GGTAC^C
MamI 2844 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 99 GCCGGC GCC^GGC
NarI 265 GGCGCC GG^CGCC
NcoI 2274 CCATGG C^CATGG
NgoMIV 99 GCCGGC G^CCGGC
PacI 2261 TTAATTAA TTAAT^TAA
PaeR7I 3426 CTCGAG C^TCGAG
PflMI 3877 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 3029 GAAGAGC GCTCTTC(1/4)
SbfI 5077 CCTGCAGG CCTGCA^GG
SfoI 265 GGCGCC GGC^GCC
Sse8387I 5077 CCTGCAGG CCTGCA^GG
StuI 5074 AGGCCT AGG^CCT
SwaI 5540 ATTTAAAT ATTT^AAAT
Van91I 3877 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3654 CCAAGACTCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3426 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Asp700I 1068 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2708 GAAACTATTC
AvrII 40 CCTAGG C^CTAGG
3966 CCTAGG
BalI 3666 TGGCCA TGG^CCA
5749 TGGCCA
BbrPI 578 CACGTG CAC^GTG
5372 CACGTG
BbvCI 2624 CCTCAGC CCTCAGC(-5/-2)
2207 GCTGAGG
BlnI 40 CCTAGG C^CTAGG
3966 CCTAGG
BpmI 135 CTGGAG CTGGAG(16/14)
2028 CTGGAG
BsaAI 578 CACGTG [CT]AC^GT[GA]
5372 CACGTG
BseRI 32 GAGGAG GAGGAG(10/8)
1481 GAGGAG
BsgI 3744 CTGCAC GTGCAG(16/14)
843 GTGCAG
BsiEI 101 CGGCCG CG[GA][CT]^CG
375 CGGCCG
Bsp1407I 654 TGTACA T^GTACA
4894 TGTACA
BspLU11I 2162 ACATGT A^CATGT
4891 ACATGT
BsrGI 654 TGTACA T^GTACA
4894 TGTACA
BssSI 1051 CACGAG CACGAG(-5/-1)
3542 CACGAG
DraII 588 GGGACCT [GA]G^G[ACGT]CC[CT]
2281 GGGTCCT
EagI 101 CGGCCG C^GGCCG
375 CGGCCG
EclXI 101 CGGCCG C^GGCCG
375 CGGCCG
Eco52I 101 CGGCCG C^GGCCG
375 CGGCCG
EcoO109I 588 GGGACCT [GA]G^G[ACGT]CC[CT]
2281 GGGTCCT
EcoRV 1013 GATATC GAT^ATC
2848 GATATC
EcoT22I 933 ATGCAT ATGCA^T
5670 ATGCAT
HgaI 399 GACGC GACGC(5/10)
4312 GACGC
MluNI 3666 TGGCCA TGG^CCA
5749 TGGCCA
MscI 3666 TGGCCA TGG^CCA
5749 TGGCCA
NsiI 933 ATGCAT ATGCA^T
5670 ATGCAT
PmaCI 578 CACGTG CAC^GTG
5372 CACGTG
PmlI 578 CACGTG CAC^GTG
5372 CACGTG
PpuMI 588 GGGACCT [GA]G^G[AT]CC[CT]
2281 GGGTCCT
SacI 3423 GAGCTC GAGCT^C
3446 GAGCTC
SmaI 340 CCCGGG CCC^GGG
363 CCCGGG
SspBI 654 TGTACA T^GTACA
4894 TGTACA
XbaI 793 TCTAGA T^CTAGA
3151 TCTAGA
XmaCI 340 CCCGGG C^CCGGG
363 CCCGGG
XmaI 340 CCCGGG C^CCGGG
363 CCCGGG
XmnI 1068 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2708 GAAACTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AseI AsnI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BclI BcnI BfaI BfrI BglI
BglII BmyI BpuAI BsaHI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RcaI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII