##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AccI        AclI        AflII       Aor51HI     AscI        
AseI        AsnI        BbeI        BfrI        BlpI        
Bpu1102I    BsaBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       Bst1107I    BstBI       BstZ17I     
CelII       ClaI        DraI        Eco47III    EcoRI       
EcoRV       FseI        HpaI        KasI        MamI        
MfeI        MluI        MunI        NarI        NheI        
NotI        NruI        NspI        NspV        PacI        
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        SalI        SbfI        ScaI        SfoI        
SfuI        SgrAI       SnaBI       SphI        Sse8387I    
SspBI       SwaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        3804  GACGTC               GACGT^C
AccIII       1263  TCCGGA               T^CCGGA
AgeI         4478  ACCGGT               A^CCGGT
AhdI         3334  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I       347  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3334  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         4835  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        3653  TGCGCA               TGC^GCA
BbrPI        2311  CACGTG               CAC^GTG
BglII         864  AGATCT               A^GATCT
BsaI         4106  GAGACC               GGTCTC(1/5)
BseAI        1263  TCCGGA               T^CCGGA
BsiWI         479  CGTACG               C^GTACG
BsmBI        2348  GAGACG               CGTCTC(1/5)
BsmI         3058  GCATTC               GAATGC(1/-1)
BspEI        1263  TCCGGA               T^CCGGA
BssHII        267  GCGCGC               G^CGCGC
BstAPI       2420  GCACACTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1790  GGTCACC              G^GT[ACGT]ACC
BstPI        1790  GGTCACC              G^GT[ACGT]ACC
CpoI         4435  CGGACCG              CG^G[AT]CCG
Eam1105I     3334  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1790  GGTCACC              G^GT[ACGT]ACC
EcoT22I      3772  ATGCAT               ATGCA^T
FspI         3653  TGCGCA               TGC^GCA
HindIII      1289  AAGCTT               A^AGCTT
MroI         1263  TCCGGA               T^CCGGA
NsiI         3772  ATGCAT               ATGCA^T
PflFI        4835  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        4478  ACCGGT               A^CCGGT
PmaCI        2311  CACGTG               CAC^GTG
PmlI         2311  CACGTG               CAC^GTG
RsrII        4435  CGGACCG              CG^G[AT]CCG
SfiI         1437  GGCCTTGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         3415  ACTAGT               A^CTAGT
SspI         4968  AATATT               AAT^ATT
Tth111I      4835  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          326  TCTAGA               T^CTAGA
XcmI          309  CCAGGTGGTGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          347  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

Acc65I       1357  GGTACC               G^GTACC
             3605  GGTACC              
AcyI         3238  GGCGTC               G[GA]^CG[CT]C
             3804  GACGTC              
AflIII       3297  ACACGT               A^C[GA][CT]GT
             3563  ACGTGT              
Alw44I       2077  GTGCAC               G^TGCAC
             2418  GTGCAC              
ApaLI        2077  GTGCAC               G^TGCAC
             2418  GTGCAC              
Asp718I      1357  GGTACC               G^GTACC
             3605  GGTACC              
AvrII         339  CCTAGG               C^CTAGG
             1378  CCTAGG              
BcgI         4265  CGAGGAGATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2492  GCAAGGGGCTCG        
BciVI         539  GGATAC               GTATCC(6/5)
             4525  GTATCC              
BlnI          339  CCTAGG               C^CTAGG
             1378  CCTAGG              
BsaAI        2311  CACGTG               [CT]AC^GT[GA]
             3562  TACGTG              
BsaHI        3238  GGCGTC               G[GA]^CG[CT]C
             3804  GACGTC              
BsrDI        1809  CATTGC               GCAATG(2/0)
             3896  GCAATG              
BssSI        1222  CACGAG               CACGAG(-5/-1)
             4983  CTCGTG              
DrdI         2127  GACAGCCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2734  GACTACATGGTC        
EagI          272  CGGCCG               C^GGCCG
              761  CGGCCG              
EclXI         272  CGGCCG               C^GGCCG
              761  CGGCCG              
Eco52I        272  CGGCCG               C^GGCCG
              761  CGGCCG              
EcoNI         412  CCTCTCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1867  CCTGAGGCAGG         
Hin1I        3238  GGCGTC               G[GA]^CG[CT]C
             3804  GACGTC              
HincII       4319  GTCAAC               GT[CT]^[GA]AC
             4680  GTCAAC              
HindII       4319  GTCAAC               GT[CT]^[GA]AC
             4680  GTCAAC              
KpnI         1357  GGTACC               GGTAC^C
             3605  GGTACC              
KspI          143  CCGCGG               CCGC^GG
             3496  CCGCGG              
MseI          433  TTAA                 T^TAA
             1709  TTAA                
NdeI          705  CATATG               CA^TATG
             1153  CATATG              
PaeR7I       3389  CTCGAG               C^TCGAG
             3979  CTCGAG              
SacI         4760  GAGCTC               GAGCT^C
             4853  GAGCTC              
SacII         143  CCGCGG               CCGC^GG
             3496  CCGCGG              
SexAI        2195  ACCAGGT              A^CC[AT]GGT
             4007  ACCAGGT             
SmaI         1346  CCCGGG               CCC^GGG
             1970  CCCGGG              
StuI         3463  AGGCCT               AGG^CCT
             4328  AGGCCT              
Tru9I         433  TTAA                 T^TAA
             1709  TTAA                
XhoI         3389  CTCGAG               C^TCGAG
             3979  CTCGAG              
XmaCI        1346  CCCGGG               C^CCGGG
             1970  CCCGGG              
XmaI         1346  CCCGGG               C^CCGGG
             1970  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        ApoI        
AspHI       AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbsI        BbvCI       BbvI        
BclI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII