##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AgeI        AhdI        Alw44I      ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BamHI       BbvCI       BciVI       BlnI        
BsaWI       BseAI       BsiWI       BsmBI       BspDI       
BspEI       BspLU11I    BsrDI       BstEII      BstPI       
ClaI        Eam1105I    EcoO65I     FseI        FspI        
KpnI        KspI        MluI        MroI        NheI        
NotI        NruI        PacI        PinAI       PshAI       
PshBI       Psp1406I    PvuI        SacII       SalI        
SexAI       SgrAI       SnaBI       


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccII         275  CGCG                 CG^CG
AflII        3072  CTTAAG               C^TTAAG
Aor51HI      3282  AGCGCT               AGC^GCT
BbeI         1137  GGCGCC               GGCGC^C
BbrPI        1320  CACGTG               CAC^GTG
BcgI         1488  GCAGAAGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         3072  CTTAAG               C^TTAAG
BglII         725  AGATCT               A^GATCT
BlpI         2516  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2516  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1320  CACGTG               [CT]AC^GT[GA]
BsiEI        1771  CGGCCG               CG[GA][CT]^CG
Bsp1407I     3419  TGTACA               T^GTACA
BspHI        2299  TCATGA               T^CATGA
BsrGI        3419  TGTACA               T^GTACA
BssHII        274  GCGCGC               G^CGCGC
Bst1107I     4581  GTATAC               GTA^TAC
BstAPI       2328  GCAAATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        4373  TTCGAA               TT^CGAA
BstUI         275  CGCG                 CG^CG
BstZ17I      4581  GTATAC               GTA^TAC
CelII        2516  GCTCAGC              GC^T[ACGT]AGC
CpoI         1022  CGGTCCG              CG^G[AT]CCG
DraIII       4030  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2768  GACTAGCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1771  CGGCCG               C^GGCCG
EclXI        1771  CGGCCG               C^GGCCG
Eco47III     3282  AGCGCT               AGC^GCT
Eco52I       1771  CGGCCG               C^GGCCG
EcoRI        2278  GAATTC               G^AATTC
EcoRV        3480  GATATC               GAT^ATC
HincII       4267  GTTAAC               GT[CT]^[GA]AC
HindII       4267  GTTAAC               GT[CT]^[GA]AC
HindIII      3769  AAGCTT               A^AGCTT
HpaI         4267  GTTAAC               GTT^AAC
KasI         1137  GGCGCC               G^GCGCC
MfeI          952  CAATTG               C^AATTG
MunI          952  CAATTG               C^AATTG
MvnI          275  CGCG                 CG^CG
NaeI         3158  GCCGGC               GCC^GGC
NarI         1137  GGCGCC               GG^CGCC
NcoI          518  CCATGG               C^CATGG
NdeI         3723  CATATG               CA^TATG
NgoMIV       3158  GCCGGC               G^CCGGC
NspV         4373  TTCGAA               TT^CGAA
PmaCI        1320  CACGTG               CAC^GTG
PmeI         3608  GTTTAAAC             GTTT^AAAC
PmlI         1320  CACGTG               CAC^GTG
RcaI         2299  TCATGA               T^CATGA
RsrII        1022  CGGTCCG              CG^G[AT]CCG
SbfI         2894  CCTGCAGG             CCTGCA^GG
ScaI         4115  AGTACT               AGT^ACT
SfoI         1137  GGCGCC               GGC^GCC
SfuI         4373  TTCGAA               TT^CGAA
SpeI          972  ACTAGT               A^CTAGT
SphI         2337  GCATGC               GCATG^C
Sse8387I     2894  CCTGCAGG             CCTGCA^GG
SspBI        3419  TGTACA               T^GTACA
SspI         3916  AATATT               AAT^ATT
StuI          229  AGGCCT               AGG^CCT
SwaI         3259  ATTTAAAT             ATTT^AAAT
XbaI          915  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI          51  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2205  CAGGCGCTG           
AocI         1452  CCTCAGG              CC^T[ACGT]AGG
             1638  CCTGAGG             
ApaI          790  GGGCCC               GGGCC^C
             3196  GGGCCC              
AspI          171  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1018  GACCCGGTC           
BsaBI         474  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              720  GATGAAGATC          
BsaI         1465  GAGACC               GGTCTC(1/5)
             1474  GAGACC              
Bsu36I       1452  CCTCAGG              CC^T[ACGT]AGG
             1638  CCTGAGG             
Eco81I       1452  CCTCAGG              CC^T[ACGT]AGG
             1638  CCTGAGG             
MamI          474  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              720  GATGAAGATC          
PaeR7I        279  CTCGAG               C^TCGAG
             2508  CTCGAG              
PflFI         171  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1018  GACCCGGTC           
PflMI         518  CCATGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              794  CCACCAGCTGG         
PpuMI        1759  AGGACCT              [GA]G^G[AT]CC[CT]
             3120  GGGTCCC             
PvuII         798  CAGCTG               CAG^CTG
             3313  CAGCTG              
SacI          252  GAGCTC               GAGCT^C
             2247  GAGCTC              
SfiI         2048  GGCCTTCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2868  GGCCGTGGGGGCC       
SmaI           61  CCCGGG               CCC^GGG
             1142  CCCGGG              
Tth111I       171  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1018  GACCCGGTC           
Van91I        518  CCATGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              794  CCACCAGCTGG         
XcmI         1591  CCAGGAACCACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2107  CCACTCACCTCATGG     
XhoI          279  CTCGAG               C^TCGAG
             2508  CTCGAG              
XmaCI          61  CCCGGG               C^CCGGG
             1142  CCCGGG              
XmaI           61  CCCGGG               C^CCGGG
             1142  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BclI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PleI        
PstI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI