##################################
# NO CUT ENZYMES (   31 enzymes) #
##################################

AatII       AflII       Aor51HI     AscI        AvrII       
BamHI       BciVI       BfrI        BlnI        BsiWI       
BspDI       BspHI       Bst1107I    BstZ17I     ClaI        
CpoI        DrdI        Eco47III    EcoRI       EcoRV       
HpaI        NotI        NruI        PacI        PaeR7I      
PvuI        RcaI        RsrII       SnaBI       SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I       3772  GGTACC               G^GTACC
AclI         4000  AACGTT               AA^CGTT
AgeI         3063  ACCGGT               A^CCGGT
AhdI         4652  GACTATACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI         4305  ATTAAT               AT^TAAT
AsnI         4305  ATTAAT               AT^TAAT
Asp700I      5159  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3772  GGTACC               G^GTACC
AspEI        4652  GACTATACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         476  TGCGCA               TGC^GCA
BbrPI         892  CACGTG               CAC^GTG
BcgI         2076  CGACGAGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         5898  TGATCA               T^GATCA
BglII        1883  AGATCT               A^GATCT
BsaBI         552  GATTCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        2560  GAGACG               CGTCTC(1/5)
Bsp1407I     5289  TGTACA               T^GTACA
BsrGI        5289  TGTACA               T^GTACA
BstBI        4493  TTCGAA               TT^CGAA
BstXI        4903  CCAGCCCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I     4652  GACTATACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      5127  ATGCAT               ATGCA^T
FbaI         5898  TGATCA               T^GATCA
FseI          375  GGCCGGCC             GGCCGG^CC
FspI          476  TGCGCA               TGC^GCA
HindIII      5186  AAGCTT               A^AGCTT
KpnI         3772  GGTACC               GGTAC^C
KspI         1747  CCGCGG               CCGC^GG
MamI          552  GATTCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         5018  CAATTG               C^AATTG
MluI         1920  ACGCGT               A^CGCGT
MunI         5018  CAATTG               C^AATTG
NdeI         5116  CATATG               CA^TATG
NsiI         5127  ATGCAT               ATGCA^T
NspV         4493  TTCGAA               TT^CGAA
PinAI        3063  ACCGGT               A^CCGGT
PmaCI         892  CACGTG               CAC^GTG
PmeI         5398  GTTTAAAC             GTTT^AAAC
PmlI          892  CACGTG               CAC^GTG
PshAI        2191  GACCAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4305  ATTAAT               AT^TAAT
Psp1406I     4000  AACGTT               AA^CGTT
SacII        1747  CCGCGG               CCGC^GG
SalI         2532  GTCGAC               G^TCGAC
SbfI         2574  CCTGCAGG             CCTGCA^GG
ScaI         4109  AGTACT               AGT^ACT
SexAI        1096  ACCTGGT              A^CC[AT]GGT
SfuI         4493  TTCGAA               TT^CGAA
SgrAI        2232  CACCGGCG             C[GA]^CCGG[CT]G
Sse8387I     2574  CCTGCAGG             CCTGCA^GG
SspBI        5289  TGTACA               T^GTACA
XmnI         5159  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI         2532  GTCGAC               GT^[AC][GT]AC
             5368  GTAGAC              
Alw44I        199  GTGCAC               G^TGCAC
              895  GTGCAC              
ApaLI         199  GTGCAC               G^TGCAC
              895  GTGCAC              
AspI         1549  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5976  GACCCAGTC           
BbvCI         348  GCTGAGG              CCTCAGC(-5/-2)
             3345  GCTGAGG             
BsaAI         892  CACGTG               [CT]AC^GT[GA]
             4063  TACGTG              
BsmI         2794  GAATGC               GAATGC(1/-1)
             4533  GAATGC              
BspLU11I     4039  ACATGT               A^CATGT
             5286  ACATGT              
BstEII       2428  GGTCACC              G^GT[ACGT]ACC
             4855  GGTGACC             
BstPI        2428  GGTCACC              G^GT[ACGT]ACC
             4855  GGTGACC             
BstYI        1883  AGATCT               [GA]^GATC[CT]
             4994  GGATCT              
EcoO65I      2428  GGTCACC              G^GT[ACGT]ACC
             4855  GGTGACC             
HincII       1555  GTCAAC               GT[CT]^[GA]AC
             2532  GTCGAC              
HindII       1555  GTCAAC               GT[CT]^[GA]AC
             2532  GTCGAC              
MflI         1883  AGATCT               [GA]^GATC[CT]
             4994  GGATCT              
NcoI          240  CCATGG               C^CATGG
             3904  CCATGG              
PflFI        1549  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5976  GACCCAGTC           
SapI          230  GCTCTTC              GCTCTTC(1/4)
             2867  GCTCTTC             
SmlI         1189  CTCAAG               C^T[CT][GA]AG
             1618  CTCAAG              
SpeI         5353  ACTAGT               A^CTAGT
             5970  ACTAGT              
Tth111I      1549  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5976  GACCCAGTC           
XbaI         2299  TCTAGA               T^CTAGA
             4030  TCTAGA              
XhoII        1883  AGATCT               [GA]^GATC[CT]
             4994  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  152 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
AocI        ApaI        ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbsI        BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspEI       BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssHII      BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DraIII      DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MroI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeII       NgoMIV      NheI        NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfiI        SfoI        
SmaI        SphI        SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XmaCI       XmaI