##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AviII BamHI
BbeI BbrPI BciVI BglI BlpI
Bpu1102I BsaAI BsiEI BsiWI BsmBI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I CelII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FseI FspI HaeII HpaI
KasI KpnI KspI MluI NaeI
NarI NgoMIV NotI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI SacII SbfI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2262 GACGTC GACGT^C
AccIII 3016 TCCGGA T^CCGGA
AclI 3229 AACGTT AA^CGTT
AflII 393 CTTAAG C^TTAAG
AflIII 2338 ACATGT A^C[GA][CT]GT
AocI 1843 CCTTAGG CC^T[ACGT]AGG
AvrII 789 CCTAGG C^CTAGG
BanI 2718 GGTGCC G^G[CT][GA]CC
BcgI 4127 CGATAAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 259 TGATCA T^GATCA
BfrI 393 CTTAAG C^TTAAG
BlnI 789 CCTAGG C^CTAGG
BseAI 3016 TCCGGA T^CCGGA
Bsp1407I 1150 TGTACA T^GTACA
BspEI 3016 TCCGGA T^CCGGA
BspLU11I 2338 ACATGT A^CATGT
BsrBI 4543 GAGCGG CCGCTC(-3/-3)
BsrGI 1150 TGTACA T^GTACA
BstBI 2983 TTCGAA TT^CGAA
Bsu36I 1843 CCTTAGG CC^T[ACGT]AGG
CpoI 48 CGGTCCG CG^G[AT]CCG
DraIII 664 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1843 CCTTAGG CC^T[ACGT]AGG
FbaI 259 TGATCA T^GATCA
HincII 3949 GTCGAC GT[CT]^[GA]AC
HindII 3949 GTCGAC GT[CT]^[GA]AC
MroI 3016 TCCGGA T^CCGGA
MslI 1751 CACCAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 4638 GCTAGC G^CTAGC
NruI 4307 TCGCGA TCG^CGA
NspV 2983 TTCGAA TT^CGAA
PflMI 2904 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3229 AACGTT AA^CGTT
RsrII 48 CGGTCCG CG^G[AT]CCG
SalI 3949 GTCGAC G^TCGAC
ScaI 4963 AGTACT AGT^ACT
SexAI 4312 ACCAGGT A^CC[AT]GGT
SfuI 2983 TTCGAA TT^CGAA
SspBI 1150 TGTACA T^GTACA
SspI 2289 AATATT AAT^ATT
StuI 2579 AGGCCT AGG^CCT
Van91I 2904 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5038 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 3949 GTCGAC GT^[AC][GT]AC
4607 GTCTAC
AccII 4202 CGCG CG^CG
4308 CGCG
AcyI 2262 GACGTC G[GA]^CG[CT]C
3049 GGCGTC
Alw44I 3358 GTGCAC G^TGCAC
3535 GTGCAC
AlwNI 2320 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3592 CAGACTCTG
ApaLI 3358 GTGCAC G^TGCAC
3535 GTGCAC
AspI 54 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4530 GACTCTGTC
BsaHI 2262 GACGTC G[GA]^CG[CT]C
3049 GGCGTC
BsaWI 3016 TCCGGA [AT]^CCGG[AT]
4258 ACCGGA
BspHI 3467 TCATGA T^CATGA
4322 TCATGA
BsrFI 15 ACCGGC [GA]^CCGG[CT]
3046 ACCGGC
BstUI 4202 CGCG CG^CG
4308 CGCG
BstXI 240 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
511 CCAAAATTTTGG
CfoI 1979 GCGC GCG^C
3845 GCGC
Cfr10I 15 ACCGGC [GA]^CCGG[CT]
3046 ACCGGC
DsaI 1955 CCATGG C^C[GA][CT]GG
4407 CCATGG
HgaI 2489 GCGTC GACGC(5/10)
3050 GCGTC
HhaI 1979 GCGC GCG^C
3845 GCGC
Hin1I 2262 GACGTC G[GA]^CG[CT]C
3049 GGCGTC
HinP1I 1979 GCGC G^CGC
3845 GCGC
HindIII 481 AAGCTT A^AGCTT
2428 AAGCTT
MaeII 2263 ACGT A^CGT
3230 ACGT
MfeI 552 CAATTG C^AATTG
704 CAATTG
MunI 552 CAATTG C^AATTG
704 CAATTG
MvnI 4202 CGCG CG^CG
4308 CGCG
NcoI 1955 CCATGG C^CATGG
4407 CCATGG
NdeI 1773 CATATG CA^TATG
4190 CATATG
PflFI 54 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4530 GACTCTGTC
RcaI 3467 TCATGA T^CATGA
4322 TCATGA
SacI 2993 GAGCTC GAGCT^C
4369 GAGCTC
SapI 3063 GAAGAGC GCTCTTC(1/4)
4371 GCTCTTC
SmaI 207 CCCGGG CCC^GGG
4459 CCCGGG
Tth111I 54 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4530 GACTCTGTC
XcmI 280 CCACATTGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1753 CCAATATGCTAATGG
XmaCI 207 CCCGGG C^CCGGG
4459 CCCGGG
XmaI 207 CCCGGG C^CCGGG
4459 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspDI BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I ClaI DdeI DpnII
DraI DraII EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SpeI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI