##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccII AccIII AflII AgeI
Alw44I Aor51HI ApaLI AscI AspI
AviII BbrPI BciVI BfrI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI FspI HindIII KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SbfI SfuI SgrAI SnaBI
Sse8387I SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 5900 GGTACC G^GTACC
AccI 5323 GTCGAC GT^[AC][GT]AC
AclI 4845 AACGTT AA^CGTT
AflIII 3593 ACATGT A^C[GA][CT]GT
AseI 3903 ATTAAT AT^TAAT
AsnI 3903 ATTAAT AT^TAAT
Asp700I 3454 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5900 GGTACC G^GTACC
BbeI 2213 GGCGCC GGCGC^C
BspHI 3247 TCATGA T^CATGA
BspLU11I 3593 ACATGT A^CATGT
BsrBI 453 GAGCGG CCGCTC(-3/-3)
BssSI 5344 CACGAG CACGAG(-5/-1)
EcoNI 5981 CCTAGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 2213 GGCGCC [GA]GCGC^[CT]
HgaI 1238 GACGC GACGC(5/10)
HpaI 1473 GTTAAC GTT^AAC
KasI 2213 GGCGCC G^GCGCC
KpnI 5900 GGTACC GGTAC^C
NarI 2213 GGCGCC GG^CGCC
NdeI 112 CATATG CA^TATG
NheI 5448 GCTAGC G^CTAGC
PshAI 2416 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3903 ATTAAT AT^TAAT
Psp1406I 4845 AACGTT AA^CGTT
RcaI 3247 TCATGA T^CATGA
SalI 5323 GTCGAC G^TCGAC
SexAI 3047 ACCAGGT A^CC[AT]GGT
SfiI 2503 GGCCACCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2213 GGCGCC GGC^GCC
SpeI 6589 ACTAGT A^CTAGT
XmnI 3454 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1238 GACGCC G[GA]^CG[CT]C
2213 GGCGCC
AhdI 5326 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5528 GACCAATAGTC
AspEI 5326 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5528 GACCAATAGTC
AvrII 1658 CCTAGG C^CTAGG
3921 CCTAGG
BalI 4279 TGGCCA TGG^CCA
5606 TGGCCA
BcgI 1714 CGAACTTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4596 GCACCAACATCG
BclI 2966 TGATCA T^GATCA
3947 TGATCA
BlnI 1658 CCTAGG C^CTAGG
3921 CCTAGG
BsaHI 1238 GACGCC G[GA]^CG[CT]C
2213 GGCGCC
Bsp1407I 6417 TGTACA T^GTACA
6803 TGTACA
BsrDI 783 GCAATG GCAATG(2/0)
6481 GCAATG
BsrFI 3040 ACCGGC [GA]^CCGG[CT]
5554 ACCGGC
BsrGI 6417 TGTACA T^GTACA
6803 TGTACA
CfoI 2214 GCGC GCG^C
3741 GCGC
Cfr10I 3040 ACCGGC [GA]^CCGG[CT]
5554 ACCGGC
DraI 3932 TTTAAA TTT^AAA
6403 TTTAAA
Eam1105I 5326 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5528 GACCAATAGTC
FbaI 2966 TGATCA T^GATCA
3947 TGATCA
HhaI 2214 GCGC GCG^C
3741 GCGC
Hin1I 1238 GACGCC G[GA]^CG[CT]C
2213 GGCGCC
HinP1I 2214 GCGC G^CGC
3741 GCGC
MluNI 4279 TGGCCA TGG^CCA
5606 TGGCCA
MscI 4279 TGGCCA TGG^CCA
5606 TGGCCA
SapI 6198 GAAGAGC GCTCTTC(1/4)
4888 GCTCTTC
ScaI 3599 AGTACT AGT^ACT
6666 AGTACT
SphI 1805 GCATGC GCATG^C
2005 GCATGC
SspBI 6417 TGTACA T^GTACA
6803 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI