##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BbrPI       
BcgI        BclI        BfrI        BlnI        BsaAI       
BsaBI       BsaWI       BseAI       BsiWI       BsmBI       
BspEI       BssHII      BstBI       CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRV       FbaI        FseI        FspI        HindIII     
KspI        MamI        MfeI        MroI        MunI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        SfiI        SfuI        
SgrAI       SnaBI       Sse8387I    SwaI        Tth111I     
XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcyI          628  GGCGCC               G[GA]^CG[CT]C
ApaI           73  GGGCCC               GGGCC^C
Asp700I      2360  GAATCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        5087  GGATCC               G^GATCC
BbeI          628  GGCGCC               GGCGC^C
BglI         4376  GCCTTAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1898  AGATCT               A^GATCT
BsaHI         628  GGCGCC               G[GA]^CG[CT]C
BsaI          395  GAGACC               GGTCTC(1/5)
BsmI         1680  GCATTC               GAATGC(1/-1)
Bsp1407I     4990  TGTACA               T^GTACA
BspDI        1858  ATCGAT               AT^CGAT
BspHI        2748  TCATGA               T^CATGA
BspLU11I      964  ACATGT               A^CATGT
BsrBI         148  GAGCGG               CCGCTC(-3/-3)
BsrGI        4990  TGTACA               T^GTACA
BssSI        3140  CTCGTG               CACGAG(-5/-1)
Bst1107I     1819  GTATAC               GTA^TAC
BstEII        660  GGTGACC              G^GT[ACGT]ACC
BstPI         660  GGTGACC              G^GT[ACGT]ACC
BstXI         875  CCAGCGGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1819  GTATAC               GTA^TAC
ClaI         1858  ATCGAT               AT^CGAT
EcoO65I       660  GGTGACC              G^GT[ACGT]ACC
EcoT22I       642  ATGCAT               ATGCA^T
Hin1I         628  GGCGCC               G[GA]^CG[CT]C
KasI          628  GGCGCC               G^GCGCC
MluI          903  ACGCGT               A^CGCGT
NaeI         1349  GCCGGC               GCC^GGC
NarI          628  GGCGCC               GG^CGCC
NdeI         4504  CATATG               CA^TATG
NgoMIV       1349  GCCGGC               G^CCGGC
NheI         4310  GCTAGC               G^CTAGC
NsiI          642  ATGCAT               ATGCA^T
PmeI         4106  GTTTAAAC             GTTT^AAAC
RcaI         2748  TCATGA               T^CATGA
SexAI        2412  ACCTGGT              A^CC[AT]GGT
SfoI          628  GGCGCC               GGC^GCC
SmaI         3994  CCCGGG               CCC^GGG
SpeI          272  ACTAGT               A^CTAGT
SspBI        4990  TGTACA               T^GTACA
SspI         5001  AATATT               AAT^ATT
XbaI         2060  TCTAGA               T^CTAGA
XmaCI        3994  CCCGGG               C^CCGGG
XmaI         3994  CCCGGG               C^CCGGG
XmnI         2360  GAATCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Acc65I       2263  GGTACC               G^GTACC
             2409  GGTACC              
AccI         1819  GTATAC               GT^[AC][GT]AC
             3770  GTCTAC              
AflIII        903  ACGCGT               A^C[GA][CT]GT
              964  ACATGT              
Alw44I        834  GTGCAC               G^TGCAC
             1143  GTGCAC              
ApaLI         834  GTGCAC               G^TGCAC
             1143  GTGCAC              
Asp718I      2263  GGTACC               G^GTACC
             2409  GGTACC              
BsiEI           5  CGACCG               CG[GA][CT]^CG
              788  CGACCG              
BsrDI        2158  CATTGC               GCAATG(2/0)
             4578  GCAATG              
DraIII        825  CACCTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2352  CACACCGTG           
EcoNI        1406  CCTCTCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5047  CCTTAGGCAGG         
EcoRI        4050  GAATTC               G^AATTC
             4847  GAATTC              
HincII       2419  GTTAAC               GT[CT]^[GA]AC
             3330  GTTAAC              
HindII       2419  GTTAAC               GT[CT]^[GA]AC
             3330  GTTAAC              
HpaI         2419  GTTAAC               GTT^AAC
             3330  GTTAAC              
KpnI         2263  GGTACC               GGTAC^C
             2409  GGTACC              
PpuMI        3261  GGGACCT              [GA]G^G[AT]CC[CT]
             3275  GGGACCC             
SapI          917  GAAGAGC              GCTCTTC(1/4)
             1008  GAAGAGC             
ScaI          865  AGTACT               AGT^ACT
             3544  AGTACT              
TaqI         1859  TCGA                 T^CGA
             4048  TCGA                
TthHB8I      1859  TCGA                 T^CGA
             4048  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BciVI       BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SphI        
StuI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XcmI        
XhoII