##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

Acc65I      AccIII      AgeI        AhdI        Aor51HI     
AscI        Asp700I     Asp718I     AspEI       AviII       
BamHI       BbeI        BcgI        BlpI        Bpu1102I    
BsaBI       BsaWI       BseAI       BsiWI       BspDI       
BspEI       BspLU11I    BsrBI       BssHII      BstBI       
CelII       ClaI        CpoI        Eam1105I    Eco47III    
EcoRV       FseI        FspI        HaeII       KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmeI        PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SpeI        Sse8387I    
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        4728  GACGTC               GACGT^C
AccI         4845  GTATAC               GT^[AC][GT]AC
AclI         2925  AACGTT               AA^CGTT
AcyI         4728  GACGTC               G[GA]^CG[CT]C
AflII        3459  CTTAAG               C^TTAAG
Alw44I       2746  GTGCAC               G^TGCAC
ApaI         1718  GGGCCC               GGGCC^C
ApaLI        2746  GTGCAC               G^TGCAC
AvrII        4711  CCTAGG               C^CTAGG
BbvCI        3360  GCTGAGG              CCTCAGC(-5/-2)
BclI         5237  TGATCA               T^GATCA
BfrI         3459  CTTAAG               C^TTAAG
BlnI         4711  CCTAGG               C^CTAGG
BsaHI        4728  GACGTC               G[GA]^CG[CT]C
BsiEI           2  CGGCCG               CG[GA][CT]^CG
BsrDI        1512  CATTGC               GCAATG(2/0)
BssSI        4066  CACGAG               CACGAG(-5/-1)
Bst1107I     4845  GTATAC               GTA^TAC
BstEII       4588  GGTCACC              G^GT[ACGT]ACC
BstPI        4588  GGTCACC              G^GT[ACGT]ACC
BstZ17I      4845  GTATAC               GTA^TAC
EagI            2  CGGCCG               C^GGCCG
EclXI           2  CGGCCG               C^GGCCG
Eco52I          2  CGGCCG               C^GGCCG
EcoNI         431  CCTTAGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      4588  GGTCACC              G^GT[ACGT]ACC
EcoRI        5037  GAATTC               G^AATTC
FbaI         5237  TGATCA               T^GATCA
Hin1I        4728  GACGTC               G[GA]^CG[CT]C
HpaI          727  GTTAAC               GTT^AAC
NheI         4929  GCTAGC               G^CTAGC
PshAI         206  GACCCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2925  AACGTT               AA^CGTT
SexAI        2303  ACCTGGT              A^CC[AT]GGT
SnaBI        4848  TACGTA               TAC^GTA
SphI         3982  GCATGC               GCATG^C
StuI         1868  AGGCCT               AGG^CCT
SwaI         5572  ATTTAAAT             ATTT^AAAT
XbaI         5496  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AocI          431  CCTTAGG              CC^T[ACGT]AGG
              740  CCTCAGG             
AseI         3703  ATTAAT               AT^TAAT
             5432  ATTAAT              
AsnI         3703  ATTAAT               AT^TAAT
             5432  ATTAAT              
BbrPI        2313  CACGTG               CAC^GTG
             4543  CACGTG              
BglII        1577  AGATCT               A^GATCT
             2111  AGATCT              
BsmI          629  GAATGC               GAATGC(1/-1)
             4386  GAATGC              
Bsp1407I     3607  TGTACA               T^GTACA
             5023  TGTACA              
BspHI        3335  TCATGA               T^CATGA
             3638  TCATGA              
BsrFI         522  ACCGGC               [GA]^CCGG[CT]
             1901  GCCGGT              
BsrGI        3607  TGTACA               T^GTACA
             5023  TGTACA              
BstXI        1356  CCAAGGTAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4304  CCATCATGTTGG        
Bsu36I        431  CCTTAGG              CC^T[ACGT]AGG
              740  CCTCAGG             
Cfr10I        522  ACCGGC               [GA]^CCGG[CT]
             1901  GCCGGT              
DraI         5210  TTTAAA               TTT^AAA
             5573  TTTAAA              
DraIII       1232  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5073  CACAAAGTG           
Eco81I        431  CCTTAGG              CC^T[ACGT]AGG
              740  CCTCAGG             
NcoI         1409  CCATGG               C^CATGG
             4109  CCATGG              
PmaCI        2313  CACGTG               CAC^GTG
             4543  CACGTG              
PmlI         2313  CACGTG               CAC^GTG
             4543  CACGTG              
PshBI        3703  ATTAAT               AT^TAAT
             5432  ATTAAT              
RcaI         3335  TCATGA               T^CATGA
             3638  TCATGA              
SapI         3355  GAAGAGC              GCTCTTC(1/4)
             3755  GCTCTTC             
SmaI         1715  CCCGGG               CCC^GGG
             2648  CCCGGG              
SspBI        3607  TGTACA               T^GTACA
             5023  TGTACA              
XmaCI        1715  CCCGGG               C^CCGGG
             2648  CCCGGG              
XmaI         1715  CCCGGG               C^CCGGG
             2648  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BciVI       BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII