##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AscI Asp718I AspI AviII BbrPI
BcgI BfrI BsaAI BsaBI BseAI
BsiWI BspDI BspEI BsrDI BstBI
ClaI DrdI EcoNI EcoRV EcoT22I
FspI HindIII KpnI MamI MfeI
MluI MroI MunI NheI NotI
NruI NsiI NspV PacI PflFI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI SalI SapI SbfI ScaI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 5647 GTATAC GT^[AC][GT]AC
AgeI 2732 ACCGGT A^CCGGT
AhdI 1101 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1101 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 5101 GGATCC G^GATCC
BciVI 4122 GTATCC GTATCC(6/5)
BclI 943 TGATCA T^GATCA
BglII 4878 AGATCT A^GATCT
BlpI 2944 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2944 GCTCAGC GC^T[ACGT]AGC
BsaWI 2732 ACCGGT [AT]^CCGG[AT]
Bsp1407I 4813 TGTACA T^GTACA
BsrGI 4813 TGTACA T^GTACA
BssSI 1917 CACGAG CACGAG(-5/-1)
Bst1107I 5647 GTATAC GTA^TAC
BstEII 5053 GGTGACC G^GT[ACGT]ACC
BstPI 5053 GGTGACC G^GT[ACGT]ACC
BstZ17I 5647 GTATAC GTA^TAC
CelII 2944 GCTCAGC GC^T[ACGT]AGC
CpoI 3153 CGGACCG CG^G[AT]CCG
DraIII 4602 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1101 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5053 GGTGACC G^GT[ACGT]ACC
FbaI 943 TGATCA T^GATCA
FseI 4372 GGCCGGCC GGCCGG^CC
HpaI 5572 GTTAAC GTT^AAC
NdeI 4093 CATATG CA^TATG
PaeR7I 2389 CTCGAG C^TCGAG
PinAI 2732 ACCGGT A^CCGGT
RsrII 3153 CGGACCG CG^G[AT]CCG
SgrAI 333 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 4813 TGTACA T^GTACA
SspI 1152 AATATT AAT^ATT
XhoI 2389 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 4168 ACATGT A^C[GA][CT]GT
4533 ACATGT
Aor51HI 445 AGCGCT AGC^GCT
5458 AGCGCT
AseI 1149 ATTAAT AT^TAAT
5611 ATTAAT
AsnI 1149 ATTAAT AT^TAAT
5611 ATTAAT
Asp700I 4051 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4385 GAAGCAATTC
AvrII 1283 CCTAGG C^CTAGG
3843 CCTAGG
BbeI 355 GGCGCC GGCGC^C
512 GGCGCC
BlnI 1283 CCTAGG C^CTAGG
3843 CCTAGG
BsaI 4610 GAGACC GGTCTC(1/5)
3304 GGTCTC
BsmBI 2639 CGTCTC CGTCTC(1/5)
5141 CGTCTC
BsmI 2791 GAATGC GAATGC(1/-1)
4645 GAATGC
BspHI 584 TCATGA T^CATGA
1657 TCATGA
BspLU11I 4168 ACATGT A^CATGT
4533 ACATGT
BsrBI 134 CCGCTC CCGCTC(-3/-3)
96 GAGCGG
BssHII 124 GCGCGC G^CGCGC
470 GCGCGC
BstAPI 2132 GCATCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3038 GCAGGGCTTGC
BstXI 1307 CCACACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3503 CCATCGGGCTGG
BstYI 4878 AGATCT [GA]^GATC[CT]
5101 GGATCC
Eco47III 445 AGCGCT AGC^GCT
5458 AGCGCT
HincII 3945 GTTGAC GT[CT]^[GA]AC
5572 GTTAAC
HindII 3945 GTTGAC GT[CT]^[GA]AC
5572 GTTAAC
KasI 355 GGCGCC G^GCGCC
512 GGCGCC
MaeII 2620 ACGT A^CGT
2830 ACGT
MflI 4878 AGATCT [GA]^GATC[CT]
5101 GGATCC
NarI 355 GGCGCC GG^CGCC
512 GGCGCC
PshBI 1149 ATTAAT AT^TAAT
5611 ATTAAT
RcaI 584 TCATGA T^CATGA
1657 TCATGA
SacI 1020 GAGCTC GAGCT^C
2942 GAGCTC
SexAI 3540 ACCTGGT A^CC[AT]GGT
5299 ACCTGGT
SfoI 355 GGCGCC GGC^GCC
512 GGCGCC
SmlI 58 CTTGAG C^T[CT][GA]AG
2389 CTCGAG
StuI 3139 AGGCCT AGG^CCT
3448 AGGCCT
XhoII 4878 AGATCT [GA]^GATC[CT]
5101 GGATCC
XmnI 4051 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4385 GAAGCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI