##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI AspEI AspI AviII
BbeI BbrPI BsaAI BsaHI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrGI BssHII BssSI BstBI BstUI
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoRV FseI FspI Hin1I
HpaI KasI KspI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 2234 GGTACC G^GTACC
AccI 2457 GTATAC GT^[AC][GT]AC
AcsI 5108 GAATTC [GA]^AATT[CT]
AflII 1856 CTTAAG C^TTAAG
Aor51HI 1249 AGCGCT AGC^GCT
ApoI 5108 GAATTC [GA]^AATT[CT]
Asp718I 2234 GGTACC G^GTACC
AvrII 2357 CCTAGG C^CTAGG
BcgI 1000 CGACACTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 6538 GGATAC GTATCC(6/5)
BclI 6129 TGATCA T^GATCA
BfrI 1856 CTTAAG C^TTAAG
BlnI 2357 CCTAGG C^CTAGG
BlpI 974 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 974 GCTCAGC GC^T[ACGT]AGC
BsaBI 244 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3658 CGACCG CG[GA][CT]^CG
BsmBI 4422 CGTCTC CGTCTC(1/5)
BspHI 1873 TCATGA T^CATGA
BspLU11I 2041 ACATGT A^CATGT
BsrBI 5729 GAGCGG CCGCTC(-3/-3)
BsrFI 5089 GCCGGT [GA]^CCGG[CT]
Bst1107I 2457 GTATAC GTA^TAC
BstAPI 6640 GCAGTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5920 GGTGACC G^GT[ACGT]ACC
BstPI 5920 GGTGACC G^GT[ACGT]ACC
BstZ17I 2457 GTATAC GTA^TAC
CelII 974 GCTCAGC GC^T[ACGT]AGC
CfoI 1250 GCGC GCG^C
Cfr10I 5089 GCCGGT [GA]^CCGG[CT]
DraI 6577 TTTAAA TTT^AAA
Eco47III 1249 AGCGCT AGC^GCT
EcoNI 2489 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5920 GGTGACC G^GT[ACGT]ACC
EcoRI 5108 GAATTC G^AATTC
FbaI 6129 TGATCA T^GATCA
HaeII 1249 AGCGCT [GA]GCGC^[CT]
HgaI 5966 GACGC GACGC(5/10)
HhaI 1250 GCGC GCG^C
HinP1I 1250 GCGC G^CGC
HincII 2182 GTTGAC GT[CT]^[GA]AC
HindII 2182 GTTGAC GT[CT]^[GA]AC
HindIII 1197 AAGCTT A^AGCTT
KpnI 2234 GGTACC GGTAC^C
MamI 244 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4722 CAATTG C^AATTG
MunI 4722 CAATTG C^AATTG
PaeR7I 4253 CTCGAG C^TCGAG
RcaI 1873 TCATGA T^CATGA
SexAI 5787 ACCTGGT A^CC[AT]GGT
SmaI 435 CCCGGG CCC^GGG
SphI 6096 GCATGC GCATG^C
SspI 6922 AATATT AAT^ATT
XbaI 1711 TCTAGA T^CTAGA
XhoI 4253 CTCGAG C^TCGAG
XmaCI 435 CCCGGG C^CCGGG
XmaI 435 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AflIII 1324 ACGTGT A^C[GA][CT]GT
2041 ACATGT
Asp700I 368 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1063 GAAGATCTTC
BglI 100 GCCAGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1166 GCCCCACAGGC
BsmI 186 GAATGC GAATGC(1/-1)
613 GCATTC
BspMI 3049 ACCTGC ACCTGC(4/8)
5393 GCAGGT
BsrDI 2213 CATTGC GCAATG(2/0)
6155 CATTGC
DraIII 4801 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
6928 CACTCTGTG
EcoT22I 1619 ATGCAT ATGCA^T
6556 ATGCAT
NsiI 1619 ATGCAT ATGCA^T
6556 ATGCAT
SapI 1391 GAAGAGC GCTCTTC(1/4)
5698 GCTCTTC
XmnI 368 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1063 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII