##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AscI AseI AsnI AvrII
BamHI BbeI BglI BlnI BsiWI
BsmBI BssHII BstBI BstEII BstPI
DrdI EagI EclXI Eco52I EcoO65I
FseI HindIII HpaI KasI KspI
MfeI MluI MunI NarI NdeI
NotI NruI NspV PacI PmeI
PshBI PvuI SacII SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 5722 GGTACC G^GTACC
AccIII 5292 TCCGGA T^CCGGA
AclI 3722 AACGTT AA^CGTT
AgeI 2720 ACCGGT A^CCGGT
Asp718I 5722 GGTACC G^GTACC
AspI 4026 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3689 TGCGCA TGC^GCA
BalI 557 TGGCCA TGG^CCA
BbsI 3915 GTCTTC GAAGAC(2/6)
BcgI 4691 CGAAGAAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3938 TGATCA T^GATCA
BpuAI 3915 GTCTTC GAAGAC(2/6)
BsaBI 4530 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5292 TCCGGA T^CCGGA
BsiEI 3450 CGACCG CG[GA][CT]^CG
BspDI 4813 ATCGAT AT^CGAT
BspEI 5292 TCCGGA T^CCGGA
Bst1107I 1680 GTATAC GTA^TAC
BstZ17I 1680 GTATAC GTA^TAC
ClaI 4813 ATCGAT AT^CGAT
CpoI 2722 CGGTCCG CG^G[AT]CCG
DraI 2793 TTTAAA TTT^AAA
EcoRI 1615 GAATTC G^AATTC
EcoT22I 4426 ATGCAT ATGCA^T
FbaI 3938 TGATCA T^GATCA
FspI 3689 TGCGCA TGC^GCA
KpnI 5722 GGTACC GGTAC^C
MamI 4530 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 557 TGGCCA TGG^CCA
MroI 5292 TCCGGA T^CCGGA
MscI 557 TGGCCA TGG^CCA
NaeI 725 GCCGGC GCC^GGC
NcoI 5726 CCATGG C^CATGG
NgoMIV 725 GCCGGC G^CCGGC
NheI 6449 GCTAGC G^CTAGC
NsiI 4426 ATGCAT ATGCA^T
PaeR7I 4985 CTCGAG C^TCGAG
PflFI 4026 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2720 ACCGGT A^CCGGT
Psp1406I 3722 AACGTT AA^CGTT
RsrII 2722 CGGTCCG CG^G[AT]CCG
SalI 4549 GTCGAC G^TCGAC
SmaI 5256 CCCGGG CCC^GGG
SspI 2483 AATATT AAT^ATT
Tth111I 4026 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4985 CTCGAG C^TCGAG
XmaCI 5256 CCCGGG C^CCGGG
XmaI 5256 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 4327 CGCG CG^CG
5044 CGCG
AflII 3127 CTTAAG C^TTAAG
3730 CTTAAG
AhdI 3654 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4552 GACATGCAGTC
AocI 5028 CCTGAGG CC^T[ACGT]AGG
6478 CCTCAGG
Aor51HI 2744 AGCGCT AGC^GCT
4103 AGCGCT
AspEI 3654 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4552 GACATGCAGTC
BbrPI 2032 CACGTG CAC^GTG
4788 CACGTG
BfrI 3127 CTTAAG C^TTAAG
3730 CTTAAG
BsaWI 2720 ACCGGT [AT]^CCGG[AT]
5292 TCCGGA
BsmI 581 GAATGC GAATGC(1/-1)
3878 GCATTC
BssSI 2409 CACGAG CACGAG(-5/-1)
5399 CTCGTG
BstUI 4327 CGCG CG^CG
5044 CGCG
Bsu36I 5028 CCTGAGG CC^T[ACGT]AGG
6478 CCTCAGG
DraIII 3101 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4503 CACGGAGTG
DsaI 4502 CCACGG C^C[GA][CT]GG
5726 CCATGG
Eam1105I 3654 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4552 GACATGCAGTC
Eco47III 2744 AGCGCT AGC^GCT
4103 AGCGCT
Eco81I 5028 CCTGAGG CC^T[ACGT]AGG
6478 CCTCAGG
EcoNI 2655 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3424 CCTACAGTAGG
EcoRV 2170 GATATC GAT^ATC
2323 GATATC
MvnI 4327 CGCG CG^CG
5044 CGCG
PmaCI 2032 CACGTG CAC^GTG
4788 CACGTG
PmlI 2032 CACGTG CAC^GTG
4788 CACGTG
PshAI 103 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5487 GACTGGCGTC
SacI 2812 GAGCTC GAGCT^C
4570 GAGCTC
SexAI 2366 ACCAGGT A^CC[AT]GGT
4454 ACCTGGT
StuI 3131 AGGCCT AGG^CCT
5358 AGGCCT
XbaI 2916 TCTAGA T^CTAGA
4098 TCTAGA
XcmI 4514 CCATGCTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5307 CCACCCAAACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BciVI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
SbfI ScaI ScrFI SfaNI SfcI
SmlI Sse8387I SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI