##################################
# NO CUT ENZYMES (   43 enzymes) #
##################################

Acc65I      AccIII      AclI        AscI        Asp718I     
AspI        BglII       BsaBI       BseAI       BsiEI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BssHII      ClaI        CpoI        EagI        EclXI       
Eco52I      EcoRI       HpaI        KpnI        MamI        
MluI        MroI        NotI        NruI        PacI        
PflFI       PshAI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SexAI       SnaBI       SpeI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        3643  GACGTC               GACGT^C
AhdI         1349  GACCGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI         7025  ATTAAT               AT^TAAT
AsnI         7025  ATTAAT               AT^TAAT
Asp700I      2410  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1349  GACCGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        5154  TGCGCA               TGC^GCA
BalI         1075  TGGCCA               TGG^CCA
BamHI        6668  GGATCC               G^GATCC
BcgI          708  CGATGCACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        6247  GGATAC               GTATCC(6/5)
BclI         3794  TGATCA               T^GATCA
BsaI         3367  GGTCTC               GGTCTC(1/5)
Bsp1407I     4142  TGTACA               T^GTACA
BspLU11I     5378  ACATGT               A^CATGT
BsrGI        4142  TGTACA               T^GTACA
Bst1107I     4157  GTATAC               GTA^TAC
BstBI        2399  TTCGAA               TT^CGAA
BstEII        190  GGTGACC              G^GT[ACGT]ACC
BstPI         190  GGTGACC              G^GT[ACGT]ACC
BstZ17I      4157  GTATAC               GTA^TAC
Eam1105I     1349  GACCGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       190  GGTGACC              G^GT[ACGT]ACC
EcoRV        3462  GATATC               GAT^ATC
EcoT22I      3591  ATGCAT               ATGCA^T
FbaI         3794  TGATCA               T^GATCA
FseI         1409  GGCCGGCC             GGCCGG^CC
FspI         5154  TGCGCA               TGC^GCA
KspI         2658  CCGCGG               CCGC^GG
MluNI        1075  TGGCCA               TGG^CCA
MscI         1075  TGGCCA               TGG^CCA
NdeI         3816  CATATG               CA^TATG
NsiI         3591  ATGCAT               ATGCA^T
NspV         2399  TTCGAA               TT^CGAA
PmeI         4821  GTTTAAAC             GTTT^AAAC
PshBI        7025  ATTAAT               AT^TAAT
SacII        2658  CCGCGG               CCGC^GG
SbfI         1153  CCTGCAGG             CCTGCA^GG
ScaI         5827  AGTACT               AGT^ACT
SfiI         1018  GGCCGCCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         2399  TTCGAA               TT^CGAA
SgrAI        6951  CACCGGTG             C[GA]^CCGG[CT]G
SphI         2781  GCATGC               GCATG^C
Sse8387I     1153  CCTGCAGG             CCTGCA^GG
SspBI        4142  TGTACA               T^GTACA
XcmI         2836  CCACAGCACCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2410  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AgeI         3832  ACCGGT               A^CCGGT
             6952  ACCGGT              
Alw44I        434  GTGCAC               G^TGCAC
             4707  GTGCAC              
Aor51HI       769  AGCGCT               AGC^GCT
             2447  AGCGCT              
ApaLI         434  GTGCAC               G^TGCAC
             4707  GTGCAC              
AvrII        3443  CCTAGG               C^CTAGG
             4029  CCTAGG              
BbeI         1159  GGCGCC               GGCGC^C
             4518  GGCGCC              
BglI         1019  GCCGCCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1951  GCCCTACCGGC         
BlnI         3443  CCTAGG               C^CTAGG
             4029  CCTAGG              
BsmBI        2566  CGTCTC               CGTCTC(1/5)
             1607  GAGACG              
BsrDI        4653  CATTGC               GCAATG(2/0)
             3431  GCAATG              
DrdI         1467  GACCAAACCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2993  GACACGTCCGTC        
Eco47III      769  AGCGCT               AGC^GCT
             2447  AGCGCT              
EcoNI        1897  CCTCCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5455  CCTACTTGAGG         
HgaI         1609  GACGC                GACGC(5/10)
             2332  GACGC               
KasI         1159  GGCGCC               G^GCGCC
             4518  GGCGCC              
MfeI         3653  CAATTG               C^AATTG
             6050  CAATTG              
MunI         3653  CAATTG               C^AATTG
             6050  CAATTG              
NarI         1159  GGCGCC               GG^CGCC
             4518  GGCGCC              
NcoI          132  CCATGG               C^CATGG
             1234  CCATGG              
NheI         2716  GCTAGC               G^CTAGC
             5719  GCTAGC              
PaeR7I       1853  CTCGAG               C^TCGAG
             5329  CTCGAG              
PinAI        3832  ACCGGT               A^CCGGT
             6952  ACCGGT              
PpuMI          47  GGGTCCT              [GA]G^G[AT]CC[CT]
              650  AGGTCCC             
SapI         2851  GAAGAGC              GCTCTTC(1/4)
             3233  GAAGAGC             
SfoI         1159  GGCGCC               GGC^GCC
             4518  GGCGCC              
XhoI         1853  CTCGAG               C^TCGAG
             5329  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  142 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflII       AflIII      AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbrPI       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BfrI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PmaCI       
PmlI        PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
XmaCI       XmaI